Team:Paris Bettencourt/Results

From 2014.igem.org

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<p class=text2><img id=imge1 src="https://static.igem.org/mediawiki/2014/0/0d/Intro.png"</br>Figure 1.<b>Main idea of 'Teen spirit' </b></p>
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<h6><a class=link href="https://2014.igem.org/Team:Paris_Bettencourt/Project/Old_People_Smell">Teen Spirit</a></h6><br>
<h6><a class=link href="https://2014.igem.org/Team:Paris_Bettencourt/Project/Old_People_Smell">Teen Spirit</a></h6><br>
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<p class=text1>‘Old people smell’ is a characteristic smell of people over 40 caused by the appearance of 2-nonenal in sweat. In this project we isolated and characterized natural bacteria that can metabolize 2-nonenal and therefore attenuate its smell. These bacteria could be used in a cosmetic product to reduce 2-nonenal in body odor.</p>
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<p class=text1>‘<ul>
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<li>Isolated 4 bacterial species capable of growing on 2-nonenal as a carbon source.</li>
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<li>Determined the critical smell detection limit of 2-nonenal.</li>
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<li>Analyzed 2-nonenal tolerance and degradation in natural isolates.</li>
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Revision as of 01:59, 18 October 2014

Smell
the roses
Teen
spirit
Something
fishy
Goody
two shoes
Don't
sweat it



Smell The Roses

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Teen Spirit

  • Isolated 4 bacterial species capable of growing on 2-nonenal as a carbon source.
  • Determined the critical smell detection limit of 2-nonenal.
  • Analyzed 2-nonenal tolerance and degradation in natural isolates.

Something Fishy

  • Cloned tmm into E.coli using pSB1C3 and pSEVA315 vectors, creating a new Biobrick: BBa_K1403015.
  • Characterized and quantified the activity of TMM by a colorimetric assay.
  • Confirmed the degradation of trimethylamine by gas chromatography - mass spectrometry (GC/MS).
.

Goody Two Shoes

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Don't Sweat It

- Designed a BioBrick of agaA (main gene responsible for body odor) in pSB1C3.
- Found hotspots for genes ackA and ldh (responsible for body odor) found in human microbiome samples.
- Design CRISPRs to select for natural odorless strains.
- Made a DIY formulation of probiotic cream.

Human practice

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Interlab study

The aim of the Interlab Study was to measure GFP expression under different Anderson promoters and in different plasmids.
iGEM Paris Bettencourt team

  • - Successfully transformed E.coli NEB with BBa_I20260 (J23101-B0032-E0040-B0010-B0012) in the pSB3K3 vector - Device 1
  • - Successfully built BBa_J23101 + BBa_E0240 (B0032-E0040-B0015) in pSB1C3 backbone - Device 2
  • - Successfully built BBa_J23115 + BBa_E0240 (B0032-E0040-B0015) in pSB1C3 backbone - Device 3
  • - Sequenced and gel verified the three devices
  • - Characterized all the three devices with the OD600 and fluorescence measurements in a Tecan micro-plate reader
  • - Reported the results on the Interlab Study page of the iGEM wiki
  • - Completed the description of the BBa_I20260 - Device 1
  • - Submitted and send parts: BBa_K1403000 - Device 2 and BBa_K1403001 - Device 3 to the Biobricks registry

Collaborations

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Centre for Research and Interdisciplinarity (CRI)
Faculty of Medicine Cochin Port-Royal, South wing, 2nd floor
Paris Descartes University
24, rue du Faubourg Saint Jacques
75014 Paris, France
+33 1 44 41 25 22/25
paris-bettencourt-igem@googlegroups.com
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