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Team:Paris Bettencourt/Project/TMAU
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Revision as of 10:55, 4 October 2014
| BACKGROUND
Trimethylaminuria (TMAU) commonly called Fish Odor Syndrom, is a rare genetic disease; trimethylamine (TMA) is naturally produces in the gut by the intestinal flora but it supposed to be degraded in the liver by the FMO3 enzyme coded by a gene with the same name. The suffering patients have a mutated FMO3 gene, and TMA is excreted in sweat, saliva and urine, causing a strong fish odor. |
AIMS
tmm (trimethylamine monooxygenase) gene is a bacterial gene found in the bacteria Ruegeria pomeroyi and is similar to the human FMO3 gene. The aim of this project is to clone tmm into Corynebacterium striatum, a bacteria of the skin, and to incorporate the new strain in a probiotic cosmetic in order to cure fish odor. |
RESULTS
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BIOBRICKS
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| Introduction | Aims | ? | Results | Motivations |
Introduction
Trimethylamine (TMA) is produced in the intestine by Desulfovibrio desulfuricans thanks to the fermentation of choline. In healthy patients, the FMO3 gene allows the degradation of TMA in the liver into a non-volatile compound, TMA oxide. But a mutation in the FMO3 sequence is most of the time the cause of TMAU: TMA is not degraded and is then excreted in sweat, saliva and urine leading to a strong fish odor. The patients are otherwise healthy but the disease affect their social relationships and can lead to depression. There is no cure for this metabolic disorder but some treatments, often based on avoiding some sorts of food, tend to lower the symptoms.
Aims
The strain Ruegeria pomeroyi, a genus of the Rhodobacteraceae, produces an enzyme called trimethylamine monooxygenase thanks to the tmm gene. As FMO3, this enzyme degrades trimethylamine into trimethylamine-N-oxide but is adapted to a bacterial expression. The project aims at cloning tmm into E.coli and then into Corynebacterium striatum, one of the most common bacteria of the skin. The new strain would be integrated to the skin microbiome and would suppress the fish odor.
Results
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Motivation
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