Team:Paris Bettencourt/Project/Foot Odor

Aims and Achievement

The aim of this sub-project is to produce Bacillus subtilis, which lacks the capability of producing isovaleric acid and hence the bad foot odor.

Introduction

The aim of this sub-project is to produce Bacillus subtilis, which lacks the capability of producing isovaleric acid and hence the bad foot odor.

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Results

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Methods

We hypothesize that bad foot odor could be eradicated by perturbing the Leucine degradation pathway.
We choose leucine dehydrogenase and iso-valeryl coA to perturb the leucine degradation pathway. Our goal here is to have two different strategies to inhibit isovaleric acid production. our first strategy focus on producing B.subtilis which will not be capable of producing leucine, so that it has to utilize all the leucine it could get from the sweat for its own survival rather than the production of isovaleric acid. Our second strategy focus on developing B.subtilis strain, which will be capable of producing leucine but not isovaleric acid.