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Team:Paris Bettencourt/Notebook/Old People Smell
From 2014.igem.org
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<h5 id="date">August 8th </h5> | <h5 id="date">August 8th </h5> | ||
<p id="text">After 2 days, nothind has developped on the M9 plates. M9 media is actually really slow for the development of any strain. That is why we created a new media which would be like the real skin environment: synthetic sweat according to <a href=”https://static.igem.org/mediawiki/2014/1/15/Artificial_sweat.pdf”>this recipe</a></p> | <p id="text">After 2 days, nothind has developped on the M9 plates. M9 media is actually really slow for the development of any strain. That is why we created a new media which would be like the real skin environment: synthetic sweat according to <a href=”https://static.igem.org/mediawiki/2014/1/15/Artificial_sweat.pdf”>this recipe</a></p> | ||
| - | + | <p>Fatty acids seem to be the main source of carbon so we replaced them by 2-nonenal.</br> | |
| + | We made 2 media:</br> | ||
| + | 1) without fatty acids </br> | ||
| + | 2) without fatty acids and with 2-nonenal mixed with 10% of Tween 80. | ||
| + | |||
| + | |||
| + | </br> | ||
| + | <h5 id="date">August 6th</h5> | ||
| + | <p id="text">Text</p> | ||
</div> | </div> | ||
<div id="September"> | <div id="September"> | ||
Revision as of 12:36, 14 August 2014
Paris Bettencourt 2014
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Notebook
Old Person odor
August
August 5th
The goal is to select a bacteria able to develop on 2-nonenal. We made 2 new media:
1) M9 Minimal Media 100% Glucose
- 200mL M9 Salts
- 0.1mL CaCl2 1M
- 2mL MgSO4 1M
- 20mL Glucose 20%
- Water: bring to 1L
2) M9 Minimal Media 50% Glucose - 50% 2-Nonenal (according to the number of carbon atoms)
- 200mL M9 Salts
- 0.1mL CaCl2 1M
- 2mL MgSO4 1M
- 10mL Glucose 20%
- 10mL 2-nonenal 10,7%*
- Water: bring to 1L
3) M9 Minimal Media 100% 2-nonenal
- 200mL M9 Salts
- 0.1mL CaCl2 1M
- 2mL MgSO4 1M
- 20mL 2-nonenal 10,7%*
- Water: bring to 1L
We autoclaved 6 bottles (2 of 500mL for each)
Then, we made liquid culture of C.striatum in media 1) to try if the minima media works.
* Concentration of the 2-nonenal solution: In M9 Media, we use 20mL of Glucose 20% (200g/L). The concentration of glucose is C=200/M=1.09 mol/L. Glucose has 6 carbons while 2-nonenal has 9, so we need 2/3 of the glucose volume to have the same amount of carbon atoms. C=2/3*1.09=0.74 mol/L and then 0.74*M=103.7g/L. That's why the concentration of the 2-nonenal solution is 10.73%. We also added Tween80.
August 6th
We poured 20 plates of each of the 3 media with 350mL of an agar solution and 100mL of medium.
Then we used 3 plates of each type to culture skin bacteria from 3 spots: arm, armpit and forehead. Ursczula sampled bacteria from her skin with a loop and for each spot she diluted the bacteria in 300uL of NF Water. Then we plated 100uL of each solution in each of the 3 types of plates and incubated them at 37°C.
| M9 100% glucose | M9 50%-50% | M9 100% 2-nonenal | |
| Arm | x | x | x |
| Armpit | x | x | x |
| Forehead | x | x | x |
August 8th
After 2 days, nothind has developped on the M9 plates. M9 media is actually really slow for the development of any strain. That is why we created a new media which would be like the real skin environment: synthetic sweat according to this recipe
Fatty acids seem to be the main source of carbon so we replaced them by 2-nonenal. We made 2 media: 1) without fatty acids 2) without fatty acids and with 2-nonenal mixed with 10% of Tween 80.
August 6th
Text
September
Date 1
Text
Text
Date 2
Text
Text
October
Date 1
Text
Text
Date 2
Text
Text
