Team:Paris Bettencourt/Notebook/Old People Smell

From 2014.igem.org

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<h5 id="date">August 6th 2</h5>
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<h5 id="date">August 6th</h5>
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<p id="text">We poured 20 plates of each of the 3 media with 350mL of an agar solution and 100mL of medium.
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<p id="text">We poured 20 plates of each of the 3 media with 350mL of an agar solution and 100mL of medium.</p>
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                                        </br>
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<p id="text">Then we used 3 plates of each type to culture skin bacteria from 3 spots: arm, armpit and forehead. Ursczula sampled bacteria from her skin with a loop and for each spot she diluted the bacteria in 300uL of NF Water. Then we plated 100uL of each solution in each of the 3 types of plates and incubated them at 37°C.</p>
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                                        <TABLE BORDER>
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<TR><TD></TD><TD><b>M9 100% glucose</b></TD><TD><b>M9 50%-50%</b></TD><TD><b>M9 100% 2-nonenal</b></TD></TR>
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<TR><TD><b>Arm</b></TD></TD><TD>x</TD><TD>x</TD><TD>x</TD></TR>
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<TR><TD><b>Armpit</b></TD><TD>x</TD><TD>x</TD><TD>x</TD></TR>
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<TR><TD><b>Forehead</b></TD<TD>x</TD><TD>x</TD><TD>x</TD></TR>
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</TABLE>
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<p id="text">Text</p>
 
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Revision as of 09:53, 14 August 2014

Paris Bettencourt 2014



Notebook

Old Person odor

August

August 5th 1

The goal is to select a bacteria able to develop on 2-nonenal. We made 2 new media:


1) M9 Minimal Media 100% Glucose

  • 200mL M9 Salts
  • 0.1mL CaCl2 1M
  • 2mL MgSO4 1M
  • 20mL Glucose 20%
  • Water: bring to 1L

2) M9 Minimal Media 50% Glucose - 50% 2-Nonenal (according to the number of carbon atoms)

  • 200mL M9 Salts
  • 0.1mL CaCl2 1M
  • 2mL MgSO4 1M
  • 10mL Glucose 20%
  • 10mL 2-nonenal 10,7%*
  • Water: bring to 1L

3) M9 Minimal Media 100% 2-nonenal

  • 200mL M9 Salts
  • 0.1mL CaCl2 1M
  • 2mL MgSO4 1M
  • 20mL 2-nonenal 10,7%*
  • Water: bring to 1L

We autoclaved 6 bottles (2 of 500mL for each)

Then, we made liquid culture of C.striatum in media 1) to try if the minima media works.


* Concentration of the 2-nonenal solution: In M9 Media, we use 20mL of Glucose 20% (200g/L). The concentration of glucose is C=200/M=1.09 mol/L. Glucose has 6 carbons while 2-nonenal has 9, so we need 2/3 of the glucose volume to have the same amount of carbon atoms. C=2/3*1.09=0.74 mol/L and then 0.74*M=103.7g/L. That's why the concentration of the 2-nonenal solution is 10.73%. We also added Tween80.

August 6th

We poured 20 plates of each of the 3 media with 350mL of an agar solution and 100mL of medium.


Then we used 3 plates of each type to culture skin bacteria from 3 spots: arm, armpit and forehead. Ursczula sampled bacteria from her skin with a loop and for each spot she diluted the bacteria in 300uL of NF Water. Then we plated 100uL of each solution in each of the 3 types of plates and incubated them at 37°C.

M9 100% glucoseM9 50%-50%M9 100% 2-nonenal
Armxxx
Armpitxxx
Foreheadxxx

September

Date 1

Text

Text

Date 2

Text

Text


October

Date 1

Text

Text

Date 2

Text

Text