Team:Paris Bettencourt/Notebook/Old People Smell

From 2014.igem.org

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                                             <li> Water: bring to 1L</li>
                                             <li> Water: bring to 1L</li>
                                         </ul>
                                         </ul>
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                                        </br>
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<p id="text"><u> 3) M9 Minimal Media 100% 2-nonenal</u></p>
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                                        <ul>
 +
                                    <li>200mL M9 Salts</li>
 +
                                    <li>0.1mL CaCl2 1M</li>
 +
                                            <li>2mL MgSO4 1M</li>
 +
                                            <li>20mL 2-nonenal 10,7%*</li>
 +
                                            <li> Water: bring to 1L</li>
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                                        </ul>
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 +
                                        <p>We autoclaved 6 bottles (2 of 500mL for each)</p>
 +
                                        <p>Then, we made liquid culture of C.striatum in media <b>1)</b> to try if the minima media works.</p>
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<h5 id="date">Date 2</h5>
<h5 id="date">Date 2</h5>

Revision as of 09:32, 14 August 2014

Paris Bettencourt 2014



Notebook

Old Person odor

August

August 5th 1

The goal is to select a bacteria able to develop on 2-nonenal. We made 2 new media:


1) M9 Minimal Media 100% Glucose

  • 200mL M9 Salts
  • 0.1mL CaCl2 1M
  • 2mL MgSO4 1M
  • 20mL Glucose 20%
  • Water: bring to 1L

2) M9 Minimal Media 50% Glucose - 50% 2-Nonenal (according to the number of carbon atoms)

  • 200mL M9 Salts
  • 0.1mL CaCl2 1M
  • 2mL MgSO4 1M
  • 10mL Glucose 20%
  • 10mL 2-nonenal 10,7%*
  • Water: bring to 1L

3) M9 Minimal Media 100% 2-nonenal

  • 200mL M9 Salts
  • 0.1mL CaCl2 1M
  • 2mL MgSO4 1M
  • 20mL 2-nonenal 10,7%*
  • Water: bring to 1L

We autoclaved 6 bottles (2 of 500mL for each)

Then, we made liquid culture of C.striatum in media 1) to try if the minima media works.

Date 2

Text

Text


September

Date 1

Text

Text

Date 2

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October

Date 1

Text

Text

Date 2

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