Team:Paris Bettencourt/Notebook/Foot Odor

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Paris Bettencourt 2014



Foot Odor

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June

23-06-2014 1
Goal: To obtain single colonies of both Wt and mutant B.Subtilis

We received the wt (1A1) and mutant BKE24040, BKE24050, BKE24080 from BGSC on 23-06-2014. The wt strain (1A1) was on a paper strip. We received 3 strips from BGSC (Bacillus genetic stock centre). Where as the mutant strains were

Procedure

We plated two strips in two different LBA plates. The plates were free from antibiotics. We placed the strips gently on the LBA and added 1 drop of sterile H2O. After few seconds we streaked the paper strip on the LBA and discarded the strip. The LBA plates were incubated at 37° C.

Results

Good growth of the wt B.subtilis was observed followed by the overnight incubation

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July

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August

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September

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October

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