Team:Oxford/protocols/Growing Liquid Cell Cultures

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Growing Liquid Cell Cultures


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  1. Switch on the lamina flow hood and carry out steps 2 and 3 in the hood.

  2. Pipette 5ml LB broth into a 15ml Falcon tube.

  3. Inoculate the Falcon tube with strain being grown using an inoculation loop.

  4. Grow at appropriate temperature (37°C for E. coli and 30°C for Pseudomonas) with shaking overnight.