Team:Oxford/biosensor optimisation
From 2014.igem.org
(Difference between revisions)
Olivervince (Talk | contribs) |
Olivervince (Talk | contribs) |
||
Line 317: | Line 317: | ||
The top graph shows that once you get above a certain threshold value of ATC input, the steady state fluorescence of the system doesn’t change. This means that to meet the above requirement, we simply have to use an ATC input value greater than the threshold value. | The top graph shows that once you get above a certain threshold value of ATC input, the steady state fluorescence of the system doesn’t change. This means that to meet the above requirement, we simply have to use an ATC input value greater than the threshold value. | ||
<br><br> | <br><br> | ||
- | + | •The system needs to be very sensitive to changes when there is a low concentration of DCM. This is important because we want the output of our biosensor to change when there is only a very small amount of the DCM left, so that it is safe to be discarded. | |
<br><br> | <br><br> | ||
We know that when no DCM is added to the system, there will be no fluorescence response aside from the basal rate. However, the model predicts that when even a small amount of DCM is added and the system is left for a while, the system fluoresces with the saturated level of fluorescence. Therefore, we have the potential to develop a very sensitive biosensor that senses the presence of DCM, fluorescing when DCM is present and only switching off when the amount of DCM reaches a very low level. | We know that when no DCM is added to the system, there will be no fluorescence response aside from the basal rate. However, the model predicts that when even a small amount of DCM is added and the system is left for a while, the system fluoresces with the saturated level of fluorescence. Therefore, we have the potential to develop a very sensitive biosensor that senses the presence of DCM, fluorescing when DCM is present and only switching off when the amount of DCM reaches a very low level. |
Revision as of 20:56, 9 September 2014