Team:Oxford/biosensor optimisation
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• <strong>Fast response</strong> to the presence/absence of DCM.<br> | • <strong>Fast response</strong> to the presence/absence of DCM.<br> | ||
• <strong>High amplitude of output signal</strong> – it must produce enough GFP to generate a distinct signal against background noise.<br> | • <strong>High amplitude of output signal</strong> – it must produce enough GFP to generate a distinct signal against background noise.<br> | ||
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• <strong>Sensitive</strong> - it must change significantly in low concentrations of DCM. This is vital in order to achieve a response that is as close to binary as possible. The ideal system will have a very sharp decline in fluorescence at a predefined, very low value of DCM. This will ensure that the sensor will clearly indicate when the DCM mixture can be safely disposed of. <br><br> | • <strong>Sensitive</strong> - it must change significantly in low concentrations of DCM. This is vital in order to achieve a response that is as close to binary as possible. The ideal system will have a very sharp decline in fluorescence at a predefined, very low value of DCM. This will ensure that the sensor will clearly indicate when the DCM mixture can be safely disposed of. <br><br> | ||
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+ | (With regard to modelling): | ||
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+ | • <strong>Robust</strong> - it must be able to cope with variations in ATC concentration without radically altering the behaviour of the system. This is crucial because we cannot ensure that ATC concentrations throughout all the cells will be uniform in the real system. <br> | ||
By modelling the effects of parameters we are able to alter in the biological system, we were able to guide our design process to produce a biosensor that is as close to the ideal as possible without sacrificing any one criterion entirely. | By modelling the effects of parameters we are able to alter in the biological system, we were able to guide our design process to produce a biosensor that is as close to the ideal as possible without sacrificing any one criterion entirely. | ||
<br><br> | <br><br> |
Revision as of 18:42, 17 October 2014