Team:Oxford/biosensor construction

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Revision as of 08:52, 18 September 2014

Construction

In order to be able to use our model and to determine whether DcmR acts as a repressor or activator in the presence of DCM we designed the following two plasmid systems:

pOXON-2-dcmR-mCherry

Oxford iGEM 2014

pSRK-Gm-pdcmAsfGFP

Oxford iGEM 2014

Why these two plasmid backbones?

The two plasmids are partitioned during cell division by different systems, thus an equal proportion of each plasmid is maintained in each new daughter cell.

Different antibiotic resistances will allow us to select for cells that have taken up both plasmids by application of both antibiotics.

The replication origins compatible with E.coli and pseudomonas strains.

We have used two plasmids so that we can test each part in isolation before transforming them both into the same cell.

Oxford iGEM 2014

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+<li>The two plasmids are partitioned during cell division by different systems, thus an equal proportion of each plasmid is maintained in each new daughter cell. </li><br> <li>Different antibiotic resistances will allow us to select for cells that have taken up both plasmids by application of both antibiotics.</li><br> <li>The replication origins compatible with E.coli and pseudomonas strains.</li><br> <li>We have used two plasmids so that we can test each part in isolation before transforming them both into the same cell.</li>
 Oxford iGEM 2014