Team:Oxford/biosensor

From 2014.igem.org

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<h1>Predicting the mCherry fluorescence</h1>
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<h1>Developing the biosensor</h1>
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To model the first double repression, we took the fact that we won’t need to know the amount of tetR in the system and used the assumption that ATC is effectively activating the expression of dcmR, albeit parameterised by different constants. This assumption should be justified by the fact that we will be able to precisely control the addition of ATC and we will be able to measure the fluorescence of the mCherry.
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One part of our project was developing a cheap and user friendly way of detecting chlorinated solvents, focusing specifically on DCM. The engineering design team worked very closely with the biochemistry team to ensure that the construction of the biosensor went ahead as quickly and efficiently as possible. This involved characterising a previously unknown genetic circuit and then optimising the parameters that we had some form of control over to get the fastest visible response. On top of this, there is a link below to the specifics of the wet lab work and there is also a section on the physical realisation of our product.
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The links to each of these four sections are shown below.
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We modelled this first step using both deterministic and stochastic models.
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Revision as of 14:36, 9 September 2014


Biosensor


Developing the biosensor

One part of our project was developing a cheap and user friendly way of detecting chlorinated solvents, focusing specifically on DCM. The engineering design team worked very closely with the biochemistry team to ensure that the construction of the biosensor went ahead as quickly and efficiently as possible. This involved characterising a previously unknown genetic circuit and then optimising the parameters that we had some form of control over to get the fastest visible response. On top of this, there is a link below to the specifics of the wet lab work and there is also a section on the physical realisation of our product.

The links to each of these four sections are shown below.