Team:Oxford/biopolymer containment
From 2014.igem.org
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<img src="https://static.igem.org/mediawiki/2014/d/da/Oxford_polymer4.png" style="float:left;position:relative; width:50%;margin-left:25%;margin-right:25%;margin-bottom:2%;" /> | <img src="https://static.igem.org/mediawiki/2014/d/da/Oxford_polymer4.png" style="float:left;position:relative; width:50%;margin-left:25%;margin-right:25%;margin-bottom:2%;" /> | ||
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+ | By collecting the resulting 'capsules' and repeating this procedure, we built up polymer coat thicknesses up to 5mm, calculated by the difference in measured initial and final diameters (an average of 5 diameters, using 0.01 mm precision callipers).<br><br> | ||
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<img src="https://static.igem.org/mediawiki/2014/5/5e/Oxford_polymer3.jpg" style="float:left;position:relative; width:50%;margin-left:25%;margin-right:25%;margin-bottom:2%;" /> | <img src="https://static.igem.org/mediawiki/2014/5/5e/Oxford_polymer3.jpg" style="float:left;position:relative; width:50%;margin-left:25%;margin-right:25%;margin-bottom:2%;" /> | ||
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Acylation of cellulose was achieved via Acetyl Chloride esterification, based on methodology by Org. Lett., 2005, 7, 1805-1808. <br><br> | Acylation of cellulose was achieved via Acetyl Chloride esterification, based on methodology by Org. Lett., 2005, 7, 1805-1808. <br><br> | ||
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The volatility and poor visible absorption of DCM posed a challenge in reliably measuring rates of diffusion though the polymer. We decided to base our modelling on the diffusion of indigo dye from within prepared beads, collecting the following spectrophotometric absorption data (calibrated to prepared concentration standards): | The volatility and poor visible absorption of DCM posed a challenge in reliably measuring rates of diffusion though the polymer. We decided to base our modelling on the diffusion of indigo dye from within prepared beads, collecting the following spectrophotometric absorption data (calibrated to prepared concentration standards): |
Revision as of 02:03, 18 October 2014