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| + | {{Team:Oxford/templates/header}} |
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- | <meta name="generator" content="HTML Tidy for Linux (vers 25 March 2009), see www.w3.org"> | + | <div class="outer" style="overflow-y: scroll; overflow-x: hidden;"> |
- | <style type="text/css">
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- | @import url(http://fonts.googleapis.com/css?family=PT+Serif|Open+Sans:300italic,400,300);
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- | #menubar.left-menu.noprint ul li:last-child {
| + | <h1>Oxford iGEM has submitted three parts to the registry, which are described on the relevant registry pages. </h1> |
- | display:none;
| + | <br><br> |
- | }
| + | <a href="http://parts.igem.org/Part:BBa_K1446001"> |
| + | <u>Part no. 1 (BBa_K1446001)</u></a> is the gene coding for DcmA with an N-terminal pdu-microcompartment tag. |
| + | <img src="https://static.igem.org/mediawiki/parts/c/c0/BBa_k1446001.jpg" style="float:left;position:relative; width:90%; margin-right:75%;margin-bottom:2%;" /> |
| + | <br><br> |
| + | <a href="http://parts.igem.org/Part:BBa_K1446002"> |
| + | <u>Part no. 2 (BBa_K1446002)</u></a> is a superfolder GFP-gene with the N-terminal pdu-microcompartment tag. We have included a ribosome binding site upstream of the coding region as well as a his-tag at the C-terminus. This part has been used for fluorescent image analysis to show that the targeting mechanism of the N-terminal microcompartment tag works as expected. |
| + | <img src="https://static.igem.org/mediawiki/parts/1/1d/BBa_k1446002.jpg" style="float:left;position:relative; width:90%; margin-right:75%;margin-bottom:2%;" /> |
| + | <br><br> |
| + | <a href="http://parts.igem.org/Part:BBa_K1446003"> |
| + | <u>Part no. 3 (BBa_K1446003)</u></a> is the dcmR gene with a tetR promoter. We have used this part to get fluorescence data for our model described <a href="https://2014.igem.org/Team:Oxford/biosensor_characterisation#show2"> |
| + | <u>here</u></a> and showed that mCherry expression increased at higher ATC (anhydrous tetracycline) concentration, as expected. |
| + | <img src="https://static.igem.org/mediawiki/parts/8/80/BBa_k1446003.jpg" style="float:left;position:relative; width:90%; margin-right:75%;margin-bottom:2%;" /> |
| | | |
- | #search-controls {
| + | <br><br> |
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| + | Parts no. 1 and 2 belong to our bioremediation subproject and part 3 to our biosensor subproject. |
- | }
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- | <div class="outer" style="overflow-y: scroll; overflow-x: hidden;">
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- | <div id="stuff" style="float:left;position:absolute;margin-left:200px;margin-right:100px; margin-top:50px;">
| + | |
- | | + | |
- | <img src="https://static.igem.org/mediawiki/2014/0/08/Oxigemradcamcrop.jpg" style="position:absolute; width:100%;min-width:320px;z-index:-1; border-radius:15px;"/>
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- | <div style="background-color:#D9D9D9; opacity:0.7; z-index:5; margin-left:7%; Height:75px; width:35%;min-width:200px;font-size:65px;font-family:Helvetica;padding-top:5px; font-weight: 450;">
| + | |
- | <div style="background-color:white; opacity:0.7; Height:75px; width:100%;margin-top:5px:margin-bottom:5px;min-width:200px;font-size:65px;font-family:Helvetica;padding-top:5px; color:#596C8A; font-weight: 450;"><br>Parts</div>
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- | </div>
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| <br> | | <br> |
| <br> | | <br> |
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| + | <h1>Oxford iGEM have also constructed further parts but were not fully in a BioBrick compatible plasmid thus were not sent before the deadline but will be on the registry in the near future</h1> |
| + | <br> |
| + | The following two constructs are based on the intergenic region shown below from the native DCM-degrading bacterium (Methylobacterium extorquens DM4): |
| + | <img src="https://static.igem.org/mediawiki/2014/7/7f/Oxford_charac3.png" style="float:right;position:relative; width:80%; margin-right:10%;margin-bottom:2%;margin-left:10%;" /> |
| | | |
- | | + | <h1>PdcmA-sfGFP</h1> |
- | <div style="background-color:white; border-bottom-left-radius:10px;border-bottom-right-radius:10px; margin-top:50px; padding-left:10px;padding-right:10px;min-width:300px;"> | + | <img src="https://static.igem.org/mediawiki/2014/e/e8/Oxfordigem_pdcma.jpg" style="float:left;position:relative; width:90%; margin-right:75%;margin-bottom:2%;" /> |
| <br> | | <br> |
- | | + | This construct involves the bidirectional promoter that has been characterised as a new tool for molecular biologists. In this construct sfGFP is placed under control in the PdcmA direction. |
- | <table style="width:600px"> | + | Click <a href="https://2014.igem.org/Team:Oxford/biosensor_characterisation#show11"> |
- | <tr>
| + | <u>here</u></a> to see characterisation on this part in plasmid PJ404. |
- | <th>Part Identity</th>
| + | <br><br> |
- | <th>Description</th>
| + | |
- | </tr>
| + | |
- | <tr>
| + | |
- | <td>BBa_J06504</td>
| + | |
- | <td>mRFP1-derived, altered to be a BioBrick by removing a PstI site and adding BioBrick ends.</td>
| + | |
- | </tr>
| + | |
- | <tr>
| + | |
- | <td>BBa_I20260</td>
| + | |
- | <td>Measurement test kit of BBa_J23101: Inter-lab study</td>
| + | |
- | </tr> | + | |
- | <tr>
| + | |
- | <td>BBa_J23101</td>
| + | |
- | <td>Constitutive promoter family member: Inter-lab study</td>
| + | |
- | </tr> | + | |
- | <tr> | + | |
- | <td>BBa_E0240</td>
| + | |
- | <td>GFP generator: Inter-lab study</td>
| + | |
- | </tr>
| + | |
- | <tr>
| + | |
- | <td>BBa_J23115</td>
| + | |
- | <td>Constitutive promoter family member: Inter-lab study</td>
| + | |
- | </tr>
| + | |
- | | + | |
- | | + | |
- | </table>
| + | |
- | | + | |
| <br> | | <br> |
| + | <h1>PdcmR-sfGFP</h1> |
| + | <img src="https://static.igem.org/mediawiki/2014/b/ba/Oxfordigem_pdcmR.jpg" style="float:left;position:relative; width:90%; margin-right:75%;margin-bottom:2%;" /> |
| <br> | | <br> |
- | </div> | + | Similar to the construct above, this involves the same bidirectional promoter with sfGFP in placed in the opposite direction under control of PdcmR. |
| + | Click <a href="https://2014.igem.org/Team:Oxford/biosensor_characterisation#show11"> |
| + | <u>here</u></a> to see characterisation on this part in plasmid PJ404. |
| + | <br> |
| + | <br> |
| + | <h1>dcmA-sfGFP</h1> |
| + | <img src="https://static.igem.org/mediawiki/parts/9/93/DcmA-sfGFP.jpg" style="float:left;position:relative; width:90%; margin-right:75%;margin-bottom:2%;" /> |
| + | <br> |
| + | For the <a href="https://2014.igem.org/Team:Oxford/codon_optimisation#show14"> |
| + | <u>analysis</u></a> of dcmA-expression we have used dcmA-fused to sfGFP in the pRSFDuet vector, which has a T7-promoter that is under lac-control. |
| + | <br><br> |
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