Team:Oxford/Parts

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Revision as of 21:22, 17 October 2014

Parts

Oxford iGEM has submitted three parts to the registry, which are described on the relevant registry pages.

Part no. 1 (BBa_K1446001) is the gene coding for DcmA with an N-terminal pdu-microcompartment tag.

Part no. 2 (BBa_K1446002) is a superfolder GFP-gene with the N-terminal pdu-microcompartment tag. We have included a ribosome binding site upstream of the coding region as well as a his-tag at the C-terminus. This part has been used for fluorescent image analysis to show that the targeting mechanism of the N-terminal microcompartment tag works as expected.

Part no. 3 (BBa_K1446003) is the dcmR gene with a tetR promoter. We have used this part to get fluorescence data for our model described here and showed that mCherry expression increased at higher ATC (anhydrous tetracycline) concentration, as expected.

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 <u>Part no. 3 (BBa_K1446003)</u></a> is the dcmR gene with a tetR promoter. We have used this part to get fluorescence data for our model described <a href="https://2014.igem.org/Team:Oxford/biosensor_characterisation#show2">
 <u>here</u></a> and showed that mCherry expression increased at higher ATC (anhydrous tetracycline) concentration, as expected.
+<img src="https://static.igem.org/mediawiki/parts/8/80/BBa_k1446003.jpg" style="float:left;position:relative; width:90%; margin-right:75%;margin-bottom:2%;" />
 <br><br
 Parts no. 1 and 2 belong to our bioremediation subproject and part 3 to our biosensor subproject.