Team:NYMU-Taipei/project/3c

From 2014.igem.org

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       <h>Cohesion</h>
       <h>Cohesion</h>
       <h1>Purpose</h1>
       <h1>Purpose</h1>
-
       <p>To facilitate the efficiency and effect of killing part and anti-biofilm part, we design the cohesion module. It could attach our modified E. coli to the surface of the S. mutans, so that we could shorten the distance between our helper E.coli and our targeted enemy, S. mutans, and evaluate the efficiency of other mechanism, the “Completion” part in our project.</p><br>
+
       <p>To facilitate the efficiency of our killing and anti-biofilm parts, we designed a cohesion module. The cohesion module will attach our modified E. coli to the surface of the S. mutans, so we can shorten the distance between our helper E.coli and our targeted enemy, S. mutans. Then, we can evaluate the efficiency of the other mechanism, the “Completion” part in our project.</p><br>
-
       <p>For Completion-killing part, when the helper E. coli received the signal send by modified phage, the antibiotic secreted E. coli by can kill the S. mutans faster if they are close to each other. Additionally, for Completion-antibiofilm part, E. coli can sense the increase of biofilm formation in a shorter time and activate the antibioflim module.In conclusion, the “Cohesion” part able the E. coli to serve like supervisors around S. mutans and shorten the time and distance of attacking.</p>
+
       <p>For the Completion-killing part, when the helper E. coli received the signal sent by modified phage, the antibiotic secreted E. coli can kill the S. mutans faster if they are closer to each other. Additionally, E. coli can sense the increase of biofilm formation in a shorter period of time and thus activate the antibioflim module.In conclusion, the “Cohesion” part allows the E. coli to serve as a supervisor around S. mutans and reduces the time and distance of the attacks.</p>
       <h1>Background</h1>
       <h1>Background</h1>
-
       <p>To make E. coli a supervisor to control the population of S. mutans, we have to find anchor to attach E. coli to S. mutans. Thus, we take advantage of CSP. CSP is a kind of quorum sensing pheromone, which enables Streptococcus mutans to alter their gene expression when the critical density of cell population is reached. Recent studies showed that density-dependent quorum sensing (QS) system primarily comprised of the Competence Stimulating Peptide (CSP) and the ComD/ComE two-component signal transduction system. In addition to biofilm formation, the CSP-mediated QS system in S. mutans also affects its acidogenicity, aciduricity, genetic transformation and bacteriocin production. Most importantly,CSP is specificly secreted by S. mutans, which is the reason we choose it as anchor targeting S. mutans.</p>
+
       <p>In order to make E. coli a supervisor of the population of S. mutans, we have to find an anchor to attach E. coli to S. mutans. Thus, we take advantage of the CSP. CSP is a kind of quorum sensing pheromone, which enables Streptococcus mutans to alter their gene expression when the critical density of cell population is reached. Recent studies showed that density-dependent quorum sensing (QS) system is primarily comprised of the Competence Stimulating Peptide (CSP) and the ComD/ComE two-component signal transduction system. In addition to biofilm formation, the CSP-mediated QS system in S. mutans also affects its acidogenicity, aciduricity, genetic transformation and bacteriocin production. Most importantly,CSP is specificly secreted by S. mutans, which is the reason why we chose it as anchor targeting S. mutans.</p>
       <h1>Design</h1>
       <h1>Design</h1>
-
       <p>We use competence stimulating peptide (CSP), as a targeting material to attach E. coli to the surface of Streptococcus mutans. To do so, we use Surface display protein INPNC (designed by <a href="https://2011.igem.org/Team:Edinburgh" target="_blank">2011 iGEM Edinburgh team</a>) to display CSP on the membrane of E. coli, which act as the “anchor”. CSP binds to the receptors on the surface of Streptococcus mutans, and so is to our engineered helper E. coli.</p>
+
       <p>We use competence stimulating peptide (CSP) as a targeting material to attach E. coli to the surface of Streptococcus mutans. To do so, we utilize Surface display protein INPNC (designed by <a href="https://2011.igem.org/Team:Edinburgh" target="_blank">2011 iGEM Edinburgh team</a>) to display CSP on the membrane of E. coli, which act as the “anchor”. CSP binds to both the receptors on the surface of Streptococcus mutans and our engineered helper E. coli.</p>
       <p>!fig1 not yet!</p>
       <p>!fig1 not yet!</p>
       <p>!circuit fig not yet!</p>
       <p>!circuit fig not yet!</p>

Revision as of 09:44, 26 August 2014

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Cohesion

Purpose

To facilitate the efficiency of our killing and anti-biofilm parts, we designed a cohesion module. The cohesion module will attach our modified E. coli to the surface of the S. mutans, so we can shorten the distance between our helper E.coli and our targeted enemy, S. mutans. Then, we can evaluate the efficiency of the other mechanism, the “Completion” part in our project.


For the Completion-killing part, when the helper E. coli received the signal sent by modified phage, the antibiotic secreted E. coli can kill the S. mutans faster if they are closer to each other. Additionally, E. coli can sense the increase of biofilm formation in a shorter period of time and thus activate the antibioflim module.In conclusion, the “Cohesion” part allows the E. coli to serve as a supervisor around S. mutans and reduces the time and distance of the attacks.

Background

In order to make E. coli a supervisor of the population of S. mutans, we have to find an anchor to attach E. coli to S. mutans. Thus, we take advantage of the CSP. CSP is a kind of quorum sensing pheromone, which enables Streptococcus mutans to alter their gene expression when the critical density of cell population is reached. Recent studies showed that density-dependent quorum sensing (QS) system is primarily comprised of the Competence Stimulating Peptide (CSP) and the ComD/ComE two-component signal transduction system. In addition to biofilm formation, the CSP-mediated QS system in S. mutans also affects its acidogenicity, aciduricity, genetic transformation and bacteriocin production. Most importantly,CSP is specificly secreted by S. mutans, which is the reason why we chose it as anchor targeting S. mutans.

Design

We use competence stimulating peptide (CSP) as a targeting material to attach E. coli to the surface of Streptococcus mutans. To do so, we utilize Surface display protein INPNC (designed by 2011 iGEM Edinburgh team) to display CSP on the membrane of E. coli, which act as the “anchor”. CSP binds to both the receptors on the surface of Streptococcus mutans and our engineered helper E. coli.

!fig1 not yet!

!circuit fig not yet!

Result

Reference

  1. Targeted Killing of Streptococcus mutans by a Pheromone-Guided “Smart” Antimicrobial Peptide(2006)Randal  Eckert1, Jian He2, Daniel K. Yarbrough2, Fengxia Qi2,Maxwell H. Anderson3 and Wenyuan Shi
  2. Quorum sensing and biofilm formation by Streptococcus mutans. Senadheera.(2008) D1, Cvitkovitch DG