http://2014.igem.org/wiki/index.php?title=Team:Melbourne/Protocols&feed=atom&action=history
Team:Melbourne/Protocols - Revision history
2024-03-29T00:16:09Z
Revision history for this page on the wiki
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http://2014.igem.org/wiki/index.php?title=Team:Melbourne/Protocols&diff=386397&oldid=prev
P at 02:05, 18 October 2014
2014-10-18T02:05:25Z
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P
http://2014.igem.org/wiki/index.php?title=Team:Melbourne/Protocols&diff=354166&oldid=prev
Slowe at 21:07, 17 October 2014
2014-10-17T21:07:08Z
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Slowe
http://2014.igem.org/wiki/index.php?title=Team:Melbourne/Protocols&diff=354117&oldid=prev
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margin: 0.67em 0;<br />
}<br />
<br />
/**<br />
* Address styling not present in IE 8/9, Safari 5, and Chrome.<br />
*/<br />
<br />
abbr[title] {<br />
border-bottom: 1px dotted;<br />
}<br />
<br />
/**<br />
* Address style set to `bolder` in Firefox 4+, Safari 5, and Chrome.<br />
*/<br />
<br />
b,<br />
strong {<br />
font-weight: bold;<br />
}<br />
<br />
/**<br />
* Address styling not present in Safari 5 and Chrome.<br />
*/<br />
<br />
dfn {<br />
font-style: italic;<br />
}<br />
<br />
/**<br />
* Address differences between Firefox and other browsers.<br />
*/<br />
<br />
hr {<br />
-moz-box-sizing: content-box;<br />
box-sizing: content-box;<br />
height: 0;<br />
}<br />
<br />
/**<br />
* Address styling not present in IE 8/9.<br />
*/<br />
<br />
mark {<br />
background: #ff0;<br />
color: #000;<br />
}<br />
<br />
/**<br />
* Correct font family set oddly in Safari 5 and Chrome.<br />
*/<br />
<br />
code,<br />
kbd,<br />
pre,<br />
samp {<br />
font-family: monospace, serif;<br />
font-size: 1em;<br />
}<br />
<br />
/**<br />
* Improve readability of pre-formatted text in all browsers.<br />
*/<br />
<br />
pre {<br />
white-space: pre-wrap;<br />
}<br />
<br />
/**<br />
* Set consistent quote types.<br />
*/<br />
<br />
q {<br />
quotes: "\201C" "\201D" "\2018" "\2019";<br />
}<br />
<br />
/**<br />
* Address inconsistent and variable font size in all browsers.<br />
*/<br />
<br />
small {<br />
font-size: 80%;<br />
}<br />
<br />
/**<br />
* Prevent `sub` and `sup` affecting `line-height` in all browsers.<br />
*/<br />
<br />
sub,<br />
sup {<br />
font-size: 75%;<br />
line-height: 0;<br />
position: relative;<br />
vertical-align: baseline;<br />
}<br />
<br />
sup {<br />
top: -0.5em;<br />
}<br />
<br />
sub {<br />
bottom: -0.25em;<br />
}<br />
<br />
/* ==========================================================================<br />
Embedded content<br />
========================================================================== */<br />
<br />
/**<br />
* Remove border when inside `a` element in IE 8/9.<br />
*/<br />
<br />
img {<br />
border: 0;<br />
}<br />
<br />
/**<br />
* Correct overflow displayed oddly in IE 9.<br />
*/<br />
<br />
svg:not(:root) {<br />
overflow: hidden;<br />
}<br />
<br />
/* ==========================================================================<br />
Figures<br />
========================================================================== */<br />
<br />
/**<br />
* Address margin not present in IE 8/9 and Safari 5.<br />
*/<br />
<br />
figure {<br />
margin: 0;<br />
}<br />
<br />
/* ==========================================================================<br />
Forms<br />
========================================================================== */<br />
<br />
/**<br />
* Define consistent border, margin, and padding.<br />
*/<br />
<br />
fieldset {<br />
border: 1px solid #c0c0c0;<br />
margin: 0 2px;<br />
padding: 0.35em 0.625em 0.75em;<br />
}<br />
<br />
/**<br />
* 1. Correct `color` not being inherited in IE 8/9.<br />
* 2. Remove padding so people aren't caught out if they zero out fieldsets.<br />
*/<br />
<br />
legend {<br />
border: 0; /* 1 */<br />
padding: 0; /* 2 */<br />
}<br />
<br />
/**<br />
* 1. Correct font family not being inherited in all browsers.<br />
* 2. Correct font size not being inherited in all browsers.<br />
* 3. Address margins set differently in Firefox 4+, Safari 5, and Chrome.<br />
*/<br />
<br />
button,<br />
input,<br />
select,<br />
textarea {<br />
font-family: inherit; /* 1 */<br />
font-size: 100%; /* 2 */<br />
margin: 0; /* 3 */<br />
}<br />
<br />
/**<br />
* Address Firefox 4+ setting `line-height` on `input` using `!important` in<br />
* the UA stylesheet.<br />
*/<br />
<br />
button,<br />
input {<br />
line-height: normal;<br />
}<br />
<br />
/**<br />
* Address inconsistent `text-transform` inheritance for `button` and `select`.<br />
* All other form control elements do not inherit `text-transform` values.<br />
* Correct `button` style inheritance in Chrome, Safari 5+, and IE 8+.<br />
* Correct `select` style inheritance in Firefox 4+ and Opera.<br />
*/<br />
<br />
button,<br />
select {<br />
text-transform: none;<br />
}<br />
<br />
/**<br />
* 1. Avoid the WebKit bug in Android 4.0.* where (2) destroys native `audio`<br />
* and `video` controls.<br />
* 2. Correct inability to style clickable `input` types in iOS.<br />
* 3. Improve usability and consistency of cursor style between image-type<br />
* `input` and others.<br />
*/<br />
<br />
button,<br />
html input[type="button"], /* 1 */<br />
input[type="reset"],<br />
input[type="submit"] {<br />
-webkit-appearance: button; /* 2 */<br />
cursor: pointer; /* 3 */<br />
}<br />
<br />
/**<br />
* Re-set default cursor for disabled elements.<br />
*/<br />
<br />
button[disabled],<br />
html input[disabled] {<br />
cursor: default;<br />
}<br />
<br />
/**<br />
* 1. Address box sizing set to `content-box` in IE 8/9.<br />
* 2. Remove excess padding in IE 8/9.<br />
*/<br />
<br />
input[type="checkbox"],<br />
input[type="radio"] {<br />
box-sizing: border-box; /* 1 */<br />
padding: 0; /* 2 */<br />
}<br />
<br />
/**<br />
* 1. Address `appearance` set to `searchfield` in Safari 5 and Chrome.<br />
* 2. Address `box-sizing` set to `border-box` in Safari 5 and Chrome<br />
* (include `-moz` to future-proof).<br />
*/<br />
<br />
input[type="search"] {<br />
-webkit-appearance: textfield; /* 1 */<br />
-moz-box-sizing: content-box;<br />
-webkit-box-sizing: content-box; /* 2 */<br />
box-sizing: content-box;<br />
}<br />
<br />
/**<br />
* Remove inner padding and search cancel button in Safari 5 and Chrome<br />
* on OS X.<br />
*/<br />
<br />
input[type="search"]::-webkit-search-cancel-button,<br />
input[type="search"]::-webkit-search-decoration {<br />
-webkit-appearance: none;<br />
}<br />
<br />
/**<br />
* Remove inner padding and border in Firefox 4+.<br />
*/<br />
<br />
button::-moz-focus-inner,<br />
input::-moz-focus-inner {<br />
border: 0;<br />
padding: 0;<br />
}<br />
<br />
/**<br />
* 1. Remove default vertical scrollbar in IE 8/9.<br />
* 2. Improve readability and alignment in all browsers.<br />
*/<br />
<br />
textarea {<br />
overflow: auto; /* 1 */<br />
vertical-align: top; /* 2 */<br />
}<br />
<br />
/* ==========================================================================<br />
Tables<br />
========================================================================== */<br />
<br />
/**<br />
* Remove most spacing between table cells.<br />
*/<br />
<br />
table {<br />
border-collapse: collapse;<br />
border-spacing: 0;<br />
}<br />
<br />
html, body {<br />
color: #111;<br />
background-color: transparent;<br />
width: 100%;<br />
height: 100%;<br />
margin: 0;<br />
padding: 0;<br />
display:block;<br />
line-height:normal;<br />
<br />
}<br />
<br />
h1 {<br />
font-weight:bold;<br />
font-size:52px;<br />
margin:0;<br />
text-transform:uppercase;<br />
font-family: 'Lato', sans-serif;<br />
font-weight:900;<br />
text-align:center;<br />
/*font-family: "Courier New", Courier, "Lucida Sans Typewriter", "Lucida Typewriter", monospace;*/<br />
}<br />
h2 {<br />
font-weight:bold;<br />
font-size:24px;<br />
text-transform:uppercase;<br />
font-family: 'Lato', sans-serif;<br />
font-weight:900;<br />
/*font-family: "Courier New", Courier, "Lucida Sans Typewriter", "Lucida Typewriter", monospace;*/<br />
}<br />
p {<br />
font-family: 'Lato', sans-serif;<br />
/*font-family: "Courier New", Courier, "Lucida Sans Typewriter", "Lucida Typewriter", monospace;*/<br />
}<br />
img {margin:10px;}<br />
<br />
<br />
<br />
.firstHeading { display:none;}<br />
<br />
#content {border:none !important; line-height:normal !important;}<br />
<br />
h1, h2, h3, h4 {border:none !important;}<br />
<br />
#content, #gloabalWrapper {position:static !important;}<br />
<br />
<br />
<br />
/*#menubar, .left-menu, .left-menu a {<br />
color: white !important;<br />
background-color: #555555;<br />
<br />
}*/<br />
<br />
#top-section {<br />
border:none !important;<br />
}<br />
#p-logo {display:none;}<br />
<br />
#top-section {height:0px;}<br />
<br />
#search-controls {<br />
display:none;<br />
}<br />
#footer-box {<br />
display:none;<br />
}<br />
<br />
#catlinks {<br />
display:none;<br />
}<br />
<br />
</style><br />
<img src="https://static.igem.org/mediawiki/2014/9/9b/Melbourne_Banner.png" alt="Banner" height="225" width="1000"><br />
<br />
<br />
<br />
<table width="100%" style="text-transform: uppercase; font-family: 'Lato', sans-serif;"><br />
<br />
<tr height="10px"><br />
<br />
<br />
<td align="center" height="30px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7><br />
<a href="https://2014.igem.org/Team:Melbourne"style="color:#000000">Home </a> </td><br />
<br />
<td align="center" height="30px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7><br />
<a href="https://2014.igem.org/Team:Melbourne/Team"style="color:#000000"><br />
Team </a> </td><br />
<br />
<td align="center" height="30px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7><br />
<a target="_blank" href="https://igem.org/Team.cgi?year=2014&team_name=Melbourne"style="color:#000000">Official Team Profile </a></td><br />
<br />
<td align="center" height="30px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7><br />
<a href="https://2014.igem.org/Team:Melbourne/Project"style="color:#000000"> <br />
Project</a></td><br />
<br />
<td align="center" height="30px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7><br />
<a href="https://2014.igem.org/Team:Melbourne/Notebook"style="color:#000000"><br />
Notebook</a></td><br />
<br />
<td align="center" height="30px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7><br />
<a href="https://2014.igem.org/Team:Melbourne/Protocol"style="color:#000000"> <br />
Protocol</a></td><br />
<td align="center" height="30px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7><br />
<a href="https://2014.igem.org/Team:Melbourne/Safety"style=" color:#000000"> <br />
Safety </a></td><br />
<br />
<td align="center" height="30px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7><br />
<a href="https://2014.igem.org/Team:Melbourne/Attributions"style="color:#000000"><br />
Attributions </a></td><br />
<br />
<br />
<td align="center" height="30px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7><br />
<a href="https://2014.igem.org/Team:Melbourne/Public_Outreach"style="color:#000000"><br />
Public Outreach </a></td><br />
<br />
<br />
<td align="center"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"></a> </td><br />
</tr><br />
</table><br />
<br />
<h1>PROTOCOLS<br />
</h1><br />
<p><h3>PREPARING CELLS FOR TRANSFORMATION (A)</h3></p><br />
<p><a target="_blank" href="https://static.igem.org/mediawiki/2014/6/6c/MELBOURNE_A1V1_Preparing_Competent_Cells_Protocol.pdf">A1V1 Preparing Competent Cells</a></p><br />
<p><a target="_blank" href="https://static.igem.org/mediawiki/2014/6/6c/MELBOURNE_A1V1_Preparing_Competent_Cells_Protocol.pdfA2V1_Preparation_of_Liquid_Broth_Media_Protocol.pdf">A2V1 Preparation of Liquid Broth Media</a></p><br />
<p><a target="_blank" href="https://static.igem.org/mediawiki/2014/b/b4/MELBOURNE_A3V2_Making_Agar_Plates_Protocol.pdf">A3V2 Making Agar Plates</a></p><br />
<p><a target="_blank" href="https://static.igem.org/mediawiki/2014/f/f2/MELBOURNE_A4V1_Measuring_OD_Protocol.pdf">A4V1 Measuring OD</a></p><br />
<p>&nbsp;</p><br />
<p><h3>PREPARING PLASMIDS FOR TRANSFORMATION (B)</h3></p><br />
<p><a target="_blank" href="https://static.igem.org/mediawiki/2014/6/6f/MELBOURNE_B1V2_PCR_Protocol.pdf">B1V2 PCR Protocol</a></p><br />
<p><a target="_blank" href="https://static.igem.org/mediawiki/2014/f/f5/MELBOURNE_B2V4_DNA_Gel_Electrophoresis_Protocol.pdf">B2V4 DNA Gel Electrophoresis</a></p><br />
<p><a target="_blank" href="https://static.igem.org/mediawiki/2014/a/a8/MELBOURNE_B3V3_DNA_Gel_Purification_Protocol.pdf">B3V3 DNA Gel Purification</a></p><br />
<p><a target="_blank" href="https://static.igem.org/mediawiki/2014/9/9e/MELBOURNE_B4V3_Restriction_Digestion_Protocol.pdf">B4V3 Restriction Digestion</a></p><br />
<p><a target="_blank" href="https://static.igem.org/mediawiki/2014/2/24/MELBOURNE_B5V2_Ligation.pdf">B5V2 Ligation</a></p><br />
<p><a target="_blank" href="https://static.igem.org/mediawiki/2014/3/34/MELBOURNE_B6V1_RE-Based_Colony_Screening_Protocol.pdf">B6V1 RE-Based Colony Screening</a></p><br />
<p>&nbsp;</p><br />
<p><h3>TRANSFORMATION AND PROTEIN EXPRESSION (C)</h3></p><br />
<p><a target="_blank" href="https://static.igem.org/mediawiki/2014/a/a7/MELBOURNE_C1V2_Transformation_Protocol.pdf">C1V2 Transformation</a></p><br />
<p><a target="_blank" href="https://static.igem.org/mediawiki/2014/9/98/MELBOURNE_C2V2_Protein_Expression_in_E._Coli.pdf">C2V2 Protein Expression</a></p><br />
<p>&nbsp;</p><br />
<p><h3>PROTEIN PURIFICATION (D)</h3></p><br />
<p><a target="_blank" href="https://static.igem.org/mediawiki/2014/e/e1/MELBOURNE_D1V1_Column_Purification_of_His-Tagged_Proteins.pdf">D1V1 Batch Purification of His-Tagged Proteins</a></p><br />
<p>&nbsp;</p><br />
<p><h3>PROTEIN ANALYSIS (E)</h3></p><br />
<p><a target="_blank" href="https://static.igem.org/mediawiki/2014/1/1b/MELBOURNE_E1V2_SDS-PAGE_Protocol.pdf">E1V2 SDS-PAGE</a></p><br />
<p><a target="_blank" href="https://static.igem.org/mediawiki/2014/7/79/MELBOURNE_E2V2_Coomassie_Staining_Protocol.pdf">E2V2 Coomassie Staining</a></p><br />
<p><a target="_blank" href="https://static.igem.org/mediawiki/2014/c/cb/MELBOURNE_E3V6_Western_Blotting_Protocol.pdf">E3V6 Western Blotting</a></p><br />
<p>&nbsp;</p><br />
<p><h3>OTHER (F)</h3></p><br />
<p><a target="_blank" href="https://static.igem.org/mediawiki/2014/a/a5/MELBOURNE_F1V1_pH_Meter_Protocol.pdf">F1V1 pH Meter</a></p><br />
<p><a target="_blank" href="https://static.igem.org/mediawiki/2014/0/0a/MELBOURNE_F2V2_Cleanup_Protocol.pdf">F2V2 Cleaning Up</a></p><br />
<p><a target="_blank" href="https://static.igem.org/mediawiki/2014/6/65/MELBOURNE_F3V1_Autoclave_Protocol.pdf">F3V1 Large Autoclave</a></p><br />
<p><a target="_blank" href="https://static.igem.org/mediawiki/2014/1/19/MELBOURNE_F4V1_Autoclaving_Materials_-_small_autoclave.pdf">F4V1 Small Autoclave</a></p><br />
<br />
<p>&nbsp;</p><br />
</html></div>
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