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Team:Marburg:Safety - Revision history
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Revision history for this page on the wiki
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http://2014.igem.org/wiki/index.php?title=Team:Marburg:Safety&diff=398511&oldid=prev
RomanM89 at 03:36, 18 October 2014
2014-10-18T03:36:32Z
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <a href="https://<del class="diffchange diffchange-inline">www</del>.<del class="diffchange diffchange-inline">youtube</del>.<del class="diffchange diffchange-inline">com</del>/<del class="diffchange diffchange-inline">watch?v=vKbsz4ffzYY</del>"></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <a href="https://<ins class="diffchange diffchange-inline">static</ins>.<ins class="diffchange diffchange-inline">igem</ins>.<ins class="diffchange diffchange-inline">org</ins>/<ins class="diffchange diffchange-inline">mediawiki/2014/f/fa/IGEM_Marburg_2014_-_The_SURFkiller.mp4</ins>"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/8/8e/MR_slider_surfkiller.jpg" alt="SURFkiller" style="width:100%;"/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/8/8e/MR_slider_surfkiller.jpg" alt="SURFkiller" style="width:100%;"/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </a></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </a></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <span class="caption"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <span class="caption"></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> See our short video description on <a href="https://www.youtube.com/watch?v=<del class="diffchange diffchange-inline">laNPDznCGsw</del>">YouTube</a> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> See our short video description on <a href="https://www.youtube.com/watch?v=<ins class="diffchange diffchange-inline">vKbsz4ffzYY</ins>">YouTube</a> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> or <a href="https://static.igem.org/mediawiki/2014/f/fa/IGEM_Marburg_2014_-_The_SURFkiller.mp4">download it</a>.</span></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> or <a href="https://static.igem.org/mediawiki/2014/f/fa/IGEM_Marburg_2014_-_The_SURFkiller.mp4">download it</a>.</span></div></td></tr>
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RomanM89
http://2014.igem.org/wiki/index.php?title=Team:Marburg:Safety&diff=389981&oldid=prev
RomanM89 at 02:36, 18 October 2014
2014-10-18T02:36:08Z
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><span class="caption"><b>Figure 3: The SURFkiller in the lab and in nature</b></span></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><span class="caption"><b>Figure 3: The SURFkiller in the lab and in nature<ins class="diffchange diffchange-inline">.</ins></b></span></div></td></tr>
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RomanM89
http://2014.igem.org/wiki/index.php?title=Team:Marburg:Safety&diff=389910&oldid=prev
RomanM89 at 02:35, 18 October 2014
2014-10-18T02:35:32Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2014/7/7b/MR_killswitch_1.png" alt="scheme" /></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2014/7/7b/MR_killswitch_1.png" alt="scheme" /></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><span class="caption">Figure 1: Scheme of the SURFkiller system.<br /></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><span class="caption"<ins class="diffchange diffchange-inline">><b</ins>>Figure 1: Scheme of the SURFkiller system.<ins class="diffchange diffchange-inline"></b></ins><br /></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>T4-Holin: Toxin -> lysis<br /></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>T4-Holin: Toxin -> lysis<br /></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>T4-Antiholin: Antitoxin -> prevents lysis by Holin<br /></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>T4-Antiholin: Antitoxin -> prevents lysis by Holin<br /></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>YvyD is affecting the ribosomes as well. In comparison to L5, YvyD is influencing already assembled ribosomes and inactivates them by dimerization. Those dimers are no longer capable of protein biosynthesis (Lauber et al., 2009). The expression of <i>yvyD</i> (module three) is under the control of a constitutive promoter, regulated by the TetR repressor which is produced by module one.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>YvyD is affecting the ribosomes as well. In comparison to L5, YvyD is influencing already assembled ribosomes and inactivates them by dimerization. Those dimers are no longer capable of protein biosynthesis (Lauber et al., 2009). The expression of <i>yvyD</i> (module three) is under the control of a constitutive promoter, regulated by the TetR repressor which is produced by module one.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Additional security is provided by synthesis of the antagonising toxin (T4-Holin<del class="diffchange diffchange-inline">; BBa_K112000</del>) and antitoxin (T4-Antiholin). T4-Holin from the T4 phage is lysing the cells by introducing holes into the membrane. However T4-Antiholin, also from the T4 phage, is preventing this by plugging the holes (Wang et al., 2000; Young and Bläsi, 1995). If a mutation occurs in module two, the T4-Holin will immediately lyse the cells. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Additional security is provided by synthesis of the antagonising toxin (<ins class="diffchange diffchange-inline"><html><a href="http://parts.igem.org/Part:BBa_K112000" target="_blank"></ins>T4-Holin<ins class="diffchange diffchange-inline"></a></html></ins>) and antitoxin (T4-Antiholin). T4-Holin from the T4 phage is lysing the cells by introducing holes into the membrane. However T4-Antiholin, also from the T4 phage, is preventing this by plugging the holes (Wang et al., 2000; Young and Bläsi, 1995). If a mutation occurs in module two, the T4-Holin will immediately lyse the cells. </div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2014/0/0d/MR_holin_antiholin.png" alt="scheme"/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2014/0/0d/MR_holin_antiholin.png" alt="scheme"/></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><span class="caption"><br />Figure 2: Effectiveness of Holin and Antiholin</span></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><span class="caption"><br /<ins class="diffchange diffchange-inline">><b</ins>>Figure 2: Effectiveness of Holin and Antiholin<ins class="diffchange diffchange-inline">.</b></ins></span></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><span class="caption">Figure 3: The SURFkiller in the lab and in nature</span></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><span class="caption"<ins class="diffchange diffchange-inline">><b</ins>>Figure 3: The SURFkiller in the lab and in nature<ins class="diffchange diffchange-inline"></b></ins></span></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Lauber, M.A., Running, W.E., and Reilly, J.P. (2009) B. subtilis ribosomal proteins: structural homology and post-translational modifications. J Proteome Res 8: 4193–206 http://www.ncbi.nlm.nih.gov/pubmed/19653700. Accessed October 6, 2014.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Lauber, M.A., Running, W.E., and Reilly, J.P. (2009) B. subtilis ribosomal proteins: structural homology and post-translational modifications. J Proteome Res 8: 4193–206 http://www.ncbi.nlm.nih.gov/pubmed/19653700. Accessed October 6, 2014.</div></td></tr>
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RomanM89
http://2014.igem.org/wiki/index.php?title=Team:Marburg:Safety&diff=378320&oldid=prev
RomanM89 at 00:54, 18 October 2014
2014-10-18T00:54:32Z
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <a href="https://www.youtube.com/watch?v=<del class="diffchange diffchange-inline">laNPDznCGsw</del>"></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <a href="https://www.youtube.com/watch?v=<ins class="diffchange diffchange-inline">vKbsz4ffzYY</ins>"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/8/8e/MR_slider_surfkiller.jpg" alt="SURFkiller" style="width:100%;"/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/8/8e/MR_slider_surfkiller.jpg" alt="SURFkiller" style="width:100%;"/></div></td></tr>
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RomanM89
http://2014.igem.org/wiki/index.php?title=Team:Marburg:Safety&diff=370818&oldid=prev
Domenica at 23:50, 17 October 2014
2014-10-17T23:50:43Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>SURFkiller were deleted in the organism in order to guarantee a tight control of our system. Before deleting the genes the modules presented </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>SURFkiller were deleted in the organism in order to guarantee a tight control of our system. Before deleting the genes the modules presented </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>in figure 1 were inserted via homologous recombination into the genome. First of all module two was integrated into the <i>amyE</i> locus. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>in figure 1 were inserted via homologous recombination into the genome. First of all module two was integrated into the <i>amyE</i> locus. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Afterwards module two was placed into the <i>lacA</i> locus of <i>B. subtilis</i>, followed by the insertion of both, module <del class="diffchange diffchange-inline">2 </del>and <del class="diffchange diffchange-inline">3 </del>into </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Afterwards module two was placed into the <i>lacA</i> locus of <i>B. subtilis</i>, followed by the insertion of both, module <ins class="diffchange diffchange-inline">two </ins>and <ins class="diffchange diffchange-inline">three </ins>into </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>the <i>amyE</i> locus.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>the <i>amyE</i> locus.</div></td></tr>
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Domenica
http://2014.igem.org/wiki/index.php?title=Team:Marburg:Safety&diff=359553&oldid=prev
RomanM89 at 22:04, 17 October 2014
2014-10-17T22:04:06Z
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> See our short video description <a href="https://www.youtube.com/watch?v=laNPDznCGsw"><del class="diffchange diffchange-inline">on </del>YouTube</a> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> See our short video description <ins class="diffchange diffchange-inline">on </ins><a href="https://www.youtube.com/watch?v=laNPDznCGsw">YouTube</a> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> or <a href="https://static.igem.org/mediawiki/2014/f/fa/IGEM_Marburg_2014_-_The_SURFkiller.mp4">download it</a>.</span></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> or <a href="https://static.igem.org/mediawiki/2014/f/fa/IGEM_Marburg_2014_-_The_SURFkiller.mp4">download it</a>.</span></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td></tr>
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RomanM89
http://2014.igem.org/wiki/index.php?title=Team:Marburg:Safety&diff=359359&oldid=prev
RomanM89 at 22:02, 17 October 2014
2014-10-17T22:02:11Z
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 22:02, 17 October 2014</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </a></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </a></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <span class="caption"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <span class="caption"></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> See our short video description<a href="https://www.youtube.com/watch?v=laNPDznCGsw">on YouTube</a> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> See our short video description <a href="https://www.youtube.com/watch?v=laNPDznCGsw">on YouTube</a> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> or <a href="https://static.igem.org/mediawiki/2014/f/fa/IGEM_Marburg_2014_-_The_SURFkiller.mp4">download it</a>.</span></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> or <a href="https://static.igem.org/mediawiki/2014/f/fa/IGEM_Marburg_2014_-_The_SURFkiller.mp4">download it</a>.</span></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td></tr>
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RomanM89
http://2014.igem.org/wiki/index.php?title=Team:Marburg:Safety&diff=359330&oldid=prev
RomanM89 at 22:01, 17 October 2014
2014-10-17T22:01:50Z
<p></p>
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 22:01, 17 October 2014</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </a></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </a></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <span class="caption"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <span class="caption"></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> See our short video description<a href="https://www.youtube.com/watch?v=laNPDznCGsw">on YouTube</a> or <a href="https://static.igem.org/mediawiki/2014/f/fa/IGEM_Marburg_2014_-_The_SURFkiller.mp4">download it<del class="diffchange diffchange-inline">.</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> See our short video description<a href="https://www.youtube.com/watch?v=laNPDznCGsw">on YouTube</a> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del></a>.</span></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins>or <a href="https://static.igem.org/mediawiki/2014/f/fa/IGEM_Marburg_2014_-_The_SURFkiller.mp4">download it</a>.</span></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p><p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p><p></div></td></tr>
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RomanM89
http://2014.igem.org/wiki/index.php?title=Team:Marburg:Safety&diff=359224&oldid=prev
RomanM89 at 22:00, 17 October 2014
2014-10-17T22:00:34Z
<p></p>
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 22:00, 17 October 2014</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 12:</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </a></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </a></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <span class="caption"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <span class="caption"></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> See <del class="diffchange diffchange-inline">a </del>short <a href="https://www.youtube.com/watch?v=laNPDznCGsw"></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> See <ins class="diffchange diffchange-inline">our </ins>short <ins class="diffchange diffchange-inline">video description</ins><a href="https://www.youtube.com/watch?v=laNPDznCGsw"><ins class="diffchange diffchange-inline">on YouTube</ins></a> or <a href="https://static.igem.org/mediawiki/2014/f/fa/IGEM_Marburg_2014_-_The_SURFkiller.mp4">download <ins class="diffchange diffchange-inline">it.</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> video description</del></a> or <a href="https://static.igem.org/mediawiki/2014/f/fa/IGEM_Marburg_2014_-_The_SURFkiller.mp4">download <del class="diffchange diffchange-inline">our animation</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </a>.</span></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </a>.</span></div></td></tr>
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RomanM89
http://2014.igem.org/wiki/index.php?title=Team:Marburg:Safety&diff=348153&oldid=prev
RomanM89 at 20:01, 17 October 2014
2014-10-17T20:01:05Z
<p></p>
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 20:01, 17 October 2014</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> See a short <a href="https://www.youtube.com/watch?v=laNPDznCGsw"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> See a short <a href="https://www.youtube.com/watch?v=laNPDznCGsw"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> video description</a> or <a href="https://static.igem.org/mediawiki/2014/f/fa/IGEM_Marburg_2014_-_The_SURFkiller.mp4">download our animation</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> video description</a> or <a href="https://static.igem.org/mediawiki/2014/f/fa/IGEM_Marburg_2014_-_The_SURFkiller.mp4">download our animation</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </a>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </a>.</span></div></td></tr>
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RomanM89