Team:METU Turkey safety

From 2014.igem.org

(Difference between revisions)
 
(19 intermediate revisions not shown)
Line 1: Line 1:
 +
{{:Team:METU_Turkey/Templates/Navigationbar}}
{{CSS/Main}}
{{CSS/Main}}
<html>
<html>
Line 4: Line 5:
#contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .firstHeading,.visualClear {display: none;} /*-- hides default wiki settings --*/
#contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .firstHeading,.visualClear {display: none;} /*-- hides default wiki settings --*/
</style>
</style>
 +
<!--  here ends the section that changes the default wiki template to a white full width background -->
<!--  here ends the section that changes the default wiki template to a white full width background -->
Line 9: Line 11:
<!-- beginning of your page -->
<!-- beginning of your page -->
-
<tr> <td colspan="1"></td>
 
-
<td align="right" width="162px"><a href="https://igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"></a></td>
 
-
</tr>
 
<!-- menu -->
<!-- menu -->
-
<table id="menu" width="100%"  cellspacing="0" height="135px">
+
<table id="menu" width="100%"  cellspacing="0" height="40px">
-
 
+
<tr>
-
<td  bgColor="#FFFFFF"></td>
+
-
<td valign="bottom"  width="975px" align="center" bgColor="#FFFFFF">
+
<table align="center">
<table align="center">
Line 23: Line 20:
<td width="162px" align="center" onMouseOver="this.bgColor='#FEE5AD'" onMouseOut="this.bgColor='#A1DBB2'" bgColor=#A1DBB2>  
<td width="162px" align="center" onMouseOver="this.bgColor='#FEE5AD'" onMouseOut="this.bgColor='#A1DBB2'" bgColor=#A1DBB2>  
<a href="https://2014.igem.org/Team:METU_Turkey"style="text-decoration:none;color:#1C140D">HOME </a> </td>  
<a href="https://2014.igem.org/Team:METU_Turkey"style="text-decoration:none;color:#1C140D">HOME </a> </td>  
 +
 +
<td width="162px" align="center" onMouseOver="this.bgColor='#FEE5AD'" onMouseOut="this.bgColor='#A1DBB2'" bgColor=#A1DBB2>
 +
<a href="https://2014.igem.org/Team:METU_Turkey_team" style="text-decoration:none;color:#1C140D">TEAM </a> </td>
<td width="162px" align="center" onMouseOver="this.bgColor='#FEE5AD'" onMouseOut="this.bgColor='#A1DBB2'" bgColor=#A1DBB2>  
<td width="162px" align="center" onMouseOver="this.bgColor='#FEE5AD'" onMouseOut="this.bgColor='#A1DBB2'" bgColor=#A1DBB2>  
Line 30: Line 30:
<a href="https://2014.igem.org/Team:METU_Turkey_parts" style="text-decoration:none;color:#1C140D">PARTS</a></td>  
<a href="https://2014.igem.org/Team:METU_Turkey_parts" style="text-decoration:none;color:#1C140D">PARTS</a></td>  
-
<td width="162px" align="center" onMouseOver="this.bgColor='#FEE5AD'" onMouseOut="this.bgColor='#A1DBB2'" bgColor=#A1DBB2>  
+
<td width="162px" align="center" onMouseOver="this.bgColor='#FEE5AD'" onMouseOut="this.bgColor='#A1DBB2'" bgColor=#A1DBB2>
-
<a href="https://2014.igem.org/Team:Team:METU_Turkey_team" style="text-decoration:none;color:#1C140D">TEAM </a> </td>
+
<a href="https://2014.igem.org/Team:METU_Turkey_modeling" style="text-decoration:none;color:#1C140D">MODELING </a> </td>
<td width="162px" align="center" onMouseOver="this.bgColor='#FEE5AD'" onMouseOut="this.bgColor='#A1DBB2'" bgColor=#A1DBB2>   
<td width="162px" align="center" onMouseOver="this.bgColor='#FEE5AD'" onMouseOut="this.bgColor='#A1DBB2'" bgColor=#A1DBB2>   
-
<a href="https://2014.igem.org/Team:METU_Turkey_notebook" style="text-decoration:none;color:#1C140D">NOTEBOOK </a> </td>
+
<a href="https://2014.igem.org/Team:METU_Turkey_interlabstudy" style="text-decoration:none;color:#1C140D">INTERLAB STUDY </a></td>
 +
 
 +
</tr>
 +
<tr height="50px">
 +
 
 +
 
 +
<td width="162px" align="center" onMouseOver="this.bgColor='#FEE5AD'" onMouseOut="this.bgColor='#A1DBB2'" bgColor=#A1DBB2> 
 +
<a href="https://2014.igem.org/Team:METU_Turkey_policypractice" style="text-decoration:none;color:#1C140D">POLICY&PRACTICE </a></td>
 +
 
 +
 
 +
<td width="162px" align="center" onMouseOver="this.bgColor='#FEE5AD'" onMouseOut="this.bgColor='#A1DBB2'" bgColor=#A1DBB2> 
 +
<a href="https://2014.igem.org/Team:METU_Turkey_characterization" style="text-decoration:none;color:#1C140D">CHARACTERIZATION </a></td>
 +
 
<td width="162px" align="center" onMouseOver="this.bgColor='#FEE5AD'" onMouseOut="this.bgColor='#A1DBB2'" bgColor=#A1DBB2>   
<td width="162px" align="center" onMouseOver="this.bgColor='#FEE5AD'" onMouseOut="this.bgColor='#A1DBB2'" bgColor=#A1DBB2>   
<a href="https://2014.igem.org/Team:METU_Turkey_safety" style="text-decoration:none;color:#1C140D">SAFETY </a></td>
<a href="https://2014.igem.org/Team:METU_Turkey_safety" style="text-decoration:none;color:#1C140D">SAFETY </a></td>
-
</tr>
 
 +
<td width="162px" align="center" onMouseOver="this.bgColor='#FEE5AD'" onMouseOut="this.bgColor='#A1DBB2'" bgColor=#A1DBB2> 
 +
<a href="https://2014.igem.org/Team:METU_Turkey_attributions" style="text-decoration:none;color:#1C140D">ATTRIBUTIONS </a></td>
 +
<td width="162px" align="center" onMouseOver="this.bgColor='#FEE5AD'" onMouseOut="this.bgColor='#A1DBB2'" bgColor=#A1DBB2> 
 +
<a href="https://2014.igem.org/Team:METU_Turkey_gallery" style="text-decoration:none;color:#1C140D">GALLERY </a></td>
 +
 +
<td width="162px" align="center" onMouseOver="this.bgColor='#FEE5AD'" onMouseOut="this.bgColor='#A1DBB2'" bgColor=#A1DBB2> 
 +
<a href="https://2014.igem.org/Team:METU_Turkey_protocols" style="text-decoration:none;color:#1C140D">PROTOCOLS </a></td>
 +
</tr>
</table>
</table>
</td>
</td>
-
<td  bgColor="#FFFFFF"></td>
 
</tr>
</tr>
</table>
</table>
<!-- end of menu -->
<!-- end of menu -->
 +
<table align="center" cellspacing="0" height="200px">
 +
<tr>
 +
<td align="center" width="162px"><a href="https://igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="75px"></a></td>
 +
<td align="center" width="162px"><a href="http://www.metu.edu.tr"color:#FFFFFF"><img src="https://static.igem.org/mediawiki/2014/9/91/Metulogo.jpg" width="250px"></a></td>
 +
</tr>
 +
</table>
 +
<!--  Block of content 1 -->
 +
<table width="100%"  cellspacing="0" height="500px">
 +
<tr><td  bgColor="#FEE5AD"></td>
 +
<td width="975px" align="center" bgColor="#FEE5AD" >
 +
<table  width="975px"  cellspacing="0" height="500px">
 +
<tr>
-
<!-- Page content begins -->
+
<td width="95%" bgColor="#FEE5AD"  rowspan="3" align="center">  
 +
<!-- Content goes here -->
 +
<img src="https://static.igem.org/mediawiki/2014/b/b5/Dnastrand3.png">
 +
<h1> An importat issue: Safety!</h1>
 +
<p> All projects are being conducted in lab-safe strains of E.coli and P.putida. All researchers have been trained in applicable lab safety to insure that no bacteria are inadvertently released into the environment. We have also been trained in proper handling of chemicals. In plastics projects, the actual organisms being engineered were maintained in lab conditions (cultures, bioreactors, etc.).
 +
None of the parts we made this year raise any particular safety issues that we can foresee. All of our major parts were either taken from pre-registered biobricks, None of our new parts would provide any foreseeable selective advantage in the wild. Thus, these parts would not increase bacterial survival in the case of accidental release.</p>
-
<!-- first block of three columns -->
+
<h3>Is there any possible situation against to our kill-switch mechanism?</h3>
-
<table width="100%"  cellspacing="0" height="50px">
+
<p>Our kill switch mechanism is dependent to arabinose, so our organism would die absence of it. Its basic logic is to addict the organism our wanted enviromental conditions, so it can be controlled.</p>
-
<tr><td  bgColor="#FEE5AD"></td> <td colspan="3" width="975px" bgColor="#FEE5AD" align="center"> <h3>Safety Page!</h3></td> <td  bgColor="#FEE5AD"></td> </tr>
+
 +
</td> 
-
<!-- navigation for safety page-->
 
-
<table width="100%" align="center" >
 
-
<tr>
+
<!-- This is the back to top button -->
-
<td></td>
+
</table>
-
<td align="center" width="10%" height ="35px"
+
-
onMouseOver="this.bgColor='#A1DBB2'" onMouseOut="this.bgColor='#FEE5AD'" bgColor=#FEE5AD> 
+
-
<a href="https://2014.igem.org/Team:METU_Turkey_project_pet" style="text-decoration:none;color:#000000"> PET </a> </td>
+
-
</td>
+
-
<td align="center" width="10%" height ="35px"
 
-
onMouseOver="this.bgColor='#A1DBB2'" onMouseOut="this.bgColor='#FEE5AD'" bgColor=#FEE5AD> 
 
-
<a href="https://2014.igem.org/Team:METU_Turkey_project_degredation" style="text-decoration:none;color:#000000"> Degredation </a> </td>
 
-
</td>
 
-
<td align="center" width="10%" height ="35px"
 
-
onMouseOver="this.bgColor='#A1DBB2'" onMouseOut="this.bgColor='#FEE5AD'" bgColor=#FEE5AD> 
 
-
<a href="https://2014.igem.org/Team:METU_Turkey_project_kit" style="text-decoration:none;color:#000000"> Kit </a> </td>
 
</td>
</td>
 +
<td bgColor="#FEE5AD"></td>
 +
</tr>
 +
</table>
 +
<!-- end of Block-->
-
<td align="center" width="10%" height ="35px"  
+
 
-
onMouseOver="this.bgColor='#A1DBB2'" onMouseOut="this.bgColor='#FEE5AD'" bgColor=#FEE5AD>
+
<!--  Block of content 2 -->
-
<a href="https://2014.igem.org/Team:METU_Turkey_project_references" style="text-decoration:none;color:#000000">References </a> </td>
+
<table width="100%"  cellspacing="0" height="500px">
 +
<tr>
 +
<td bgColor="#FACA66"></td>
 +
<td width="975px" align="center" bgColor="#FACA66" >
 +
 
 +
<table  width="975px"  cellspacing="0" height="500px">
 +
<tr>
 +
<td width="95%" bgColor="#FACA66" rowspan="3" align="center">  
 +
 
 +
<!-- Content goes here -->
 +
<img src="https://static.igem.org/mediawiki/2014/0/0a/DwikitemplateB_dnastrand.png">
 +
<h1> Safety Questions & Answers</h1>
 +
<h3>1.Would any of your project ideas raise safety issues in terms of:</h3>
 +
<p>RESEARCHER SAFETY? </p>
 +
<p>There are no hazardous chemicals that we used in the laboratory except for EtBr that we used in a special room for gel visualization and some buffers used in commercially available kits.
 +
<p>PUBLIC SAFETY?</p>
 +
Since all the organisms used in the wetlab are destroyed after they are done working with they pose no danger for public safety. We use E.coli strain DH5alpha which is easy to kill and all the student members who work in the wetlab are very well trained to make sure there is no unwanted contamination. This year we are also working on a kill switch which, if works properly, will eliminate any bacteria whereas they will not be able to survive without Arabinose present.
 +
<p>ENVIRONMENTAL SAFETY?</p>
 +
As mentioned above a kill switch will be available in the organism which will initiate cell death when the cell is in an environment that does not contain enough Arabinose.
 +
<h3>2. Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues?</h3>
 +
<p>We have followed all the safety procedures during the isolation process from the cells we had and we successfully isolated the DNA without any problems that will raise safety issues.</p>
 +
<h3>3.Is there a local biosafety group, committee, or review board at your institution?
 +
If yes, what does your local biosafety group think about your project?
 +
If no, which specific biosafety rules or guidelines do you have to consider in your country?</h3>
 +
<p>In our university we have a biosafety and ethical research center which monitors the safety of our projects. http://www.ueam.metu.edu.tr The members of METU Biology department are also members of National Biosafety Coordinating Committee. We are able to consult to them whenever it is necessary. Also biosafety is a very important subject for METU Department of Biology, whereas the student members are very well trained in the subject. Also the projects that we decided to work on are presented to many people in the area in order to see if they are of any concern for the biosafety regulations. All the procedures that are used in the lab are also approved procedures that are used by these people mentioned above.</p>
 +
<h3>4. Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?</h3>
 +
We believe using specific strains designed specifically for labwork will be safer. It is possible to genetically modify the organisms so that, they are not able to grow outside the lab environment. There probably is not a way to build some biobricks that can be modified in order to make them safer since their function will be the cause of safety issues. </p>
 +
 
 +
</td>
 +
 
 +
 
 +
<!-- This is the back to top section -->
 +
</table>
 +
 
</td>
</td>
 +
<td bgColor="#FACA66"></td>
 +
</tr>
 +
</table>
 +
<!-- end of Block-->
-
<td></td>
+
 
 +
 
 +
 
 +
 
 +
 
 +
<!-- This is the back to top section -->
 +
</table>
 +
</td>
 +
<td bgColor="#F45D4C"></td>
</tr>
</tr>
</table>
</table>
-
<!-- end of navigation page -->
+
<!-- end of Block-->
 +
 +
<!-- Final block of content -->
 +
<table  id="firstblock" width="60%"  cellspacing="10px" height="200px" align="center">
<tr>
<tr>
-
<td bgColor="#FFFFFF"></td>
+
<td align="center" width="162px"><a href="http://www.metu.edu.tr"> <img src="https://static.igem.org/mediawiki/2014/9/91/Metulogo.jpg" width="175px"></a></td>
-
<td width="435px" height="400px" bgColor="#FFFFFF" >
+
<td align="center" width="162px"><a href="http://odtuteknokent.com.tr/"> <img src="https://static.igem.org/mediawiki/2014/4/47/Odtuteknokent.jpg" width="175px"></a></td>
-
<h3>Project Introduction</h3>
+
-
<p>Lorem ipsum ad his scripta blandit partiendo, eum fastidii accumsan euripidis in, eum liber hendrerit an. Qui ut wisi vocibus suscipiantur, quo dicit ridens inciderint id. Quo mundi lobortis reformidans eu, legimus senserit definiebas an eos. Eu sit tincidunt incorrupte definitionem, vis mutat affert percipit cu, eirmod consectetuer signiferumque eu per. In usu latine equidem dolores. Quo no falli viris intellegam, ut fugit veritus placerat per.</p>
+
-
</td>
+
-
<td width="50px"></td>
+
<td align="center" width="162px"><a href="http://www.kardiosis.com.tr/"> <img src="https://static.igem.org/mediawiki/2014/d/d4/Kardiosis.jpg" width="175px"></a></td>
-
<td width="435px" bgColor="#FFFFFF">
+
-
<h4>Results</h4>
+
-
<ul>
+
-
<li>Result 1 - Lorem ipsum ad his scripta blandit partiendo, eum fastidii accumsan euripidis in, eum liber hendrerit an.</li>
+
-
<li>Result 2 - Lorem ipsum ad his scripta blandit partiendo, eum fastidii accumsan euripidis in, eum liber hendrerit an.</li>
+
-
<li>Result 3 - Lorem ipsum ad his scripta blandit partiendo, eum fastidii accumsan euripidis in, eum liber hendrerit an.</li>
+
-
</ul>
+
<td align="center" width="162px"><a href="http://www.etietieti.com/"> <img src="https://static.igem.org/mediawiki/2014/7/75/Eti.jpg" width="175px"></a></td>
-
</td>
+
 
 +
<td align="center"><a href="http://www.taa-ankara.org.tr/eng/index-eng.php"> <img src="https://static.igem.org/mediawiki/2014/0/00/Turkamerikan.jpg" width="175px"></a></td>
-
<td  bgColor="#FFFFFF"></td>
 
</tr>
</tr>
-
</table>
+
<tr>
-
<!-- end of first block -->
+
 +
<td align="center"><a href="http://www.biyans.com/"> <img src="https://static.igem.org/mediawiki/2014/a/a3/Biyans.PNG" width="175px"></a></td>
 +
<td align="center"><a href="http://biyoakademi.org/"> <img src="https://static.igem.org/mediawiki/2014/b/b1/Biyoakademi.png" width="175px"></a></td>
 +
<td align="center"><a href="http://www.sentegen.com/"> <img src="https://static.igem.org/mediawiki/2014/d/dd/Sentegenlogoder.PNG" width="175px"></a></td>
-
<!-- abstract-->
+
<td align="center"><a href="http://www.geneious.com/"> <img src="https://static.igem.org/mediawiki/2014/8/89/Geneious_Logo.gif" width="175px"></a></td>
-
<table width="100%" cellspacing="0" height="275px">
+
</tr>
 +
</table>
 +
<!-- Final block of content -->
 +
<table  id="firstblock" width="100%"  cellspacing="0" height="200px">
<tr>
<tr>
<td  bgColor="#FFFFFF"></td>
<td  bgColor="#FFFFFF"></td>
 +
<td width="975px" align="center" bgColor="#FFFFFF" >
-
<td width="450px"  bgColor="#FFFFFF">
+
<p> Our Supporter: </p>
-
<h4 id="abstract"> Abstract</h4>
+
<tr>
-
<p>Lorem ipsum ad his scripta blandit partiendo, eum fastidii accumsan euripidis in, eum liber hendrerit an. Qui ut wisi vocibus suscipiantur, quo dicit ridens inciderint id. Quo mundi lobortis reformidans eu, legimus senserit definiebas an eos. Eu sit tincidunt incorrupte definitionem, vis mutat affert percipit cu, eirmod consectetuer signiferumque eu per. In usu latine equidem dolores. Quo no falli viris intellegam, ut fugit veritus placerat per.</p>
+
<td
-
</td>
+
<p>
-
<td width="20px"></td>
+
<td align="center" width="262px"><a href="http://www.odtugv.org.tr/"> <img src="https://static.igem.org/mediawiki/2014/archive/6/6c/20141015225146%21Gelistirme1.PNG"></a></td>
-
<td width="450px" bgColor="#FFFFFF">
+
</p>
-
<img src="https://static.igem.org/mediawiki/2014/c/ce/Dnastrand2.png" width="400px">
+
-
<p> You can place an image here about your project</p>
+
-
</td>
+
-
<td bgColor="#FFFFFF"></td>
+
</td>
 +
<td bgColor="#FFFFFF"></td>
</tr>
</tr>
</table>
</table>
-
<!-- end of first block -->
 
</html>
</html>

Latest revision as of 01:14, 18 October 2014

Team:METU Turkey/Templates/Navigationbar

HOME TEAM PROJECT PARTS MODELING INTERLAB STUDY
POLICY&PRACTICE CHARACTERIZATION SAFETY ATTRIBUTIONS GALLERY PROTOCOLS

An importat issue: Safety!

All projects are being conducted in lab-safe strains of E.coli and P.putida. All researchers have been trained in applicable lab safety to insure that no bacteria are inadvertently released into the environment. We have also been trained in proper handling of chemicals. In plastics projects, the actual organisms being engineered were maintained in lab conditions (cultures, bioreactors, etc.). None of the parts we made this year raise any particular safety issues that we can foresee. All of our major parts were either taken from pre-registered biobricks, None of our new parts would provide any foreseeable selective advantage in the wild. Thus, these parts would not increase bacterial survival in the case of accidental release.

Is there any possible situation against to our kill-switch mechanism?

Our kill switch mechanism is dependent to arabinose, so our organism would die absence of it. Its basic logic is to addict the organism our wanted enviromental conditions, so it can be controlled.

Safety Questions & Answers

1.Would any of your project ideas raise safety issues in terms of:

RESEARCHER SAFETY?

There are no hazardous chemicals that we used in the laboratory except for EtBr that we used in a special room for gel visualization and some buffers used in commercially available kits.

PUBLIC SAFETY?

Since all the organisms used in the wetlab are destroyed after they are done working with they pose no danger for public safety. We use E.coli strain DH5alpha which is easy to kill and all the student members who work in the wetlab are very well trained to make sure there is no unwanted contamination. This year we are also working on a kill switch which, if works properly, will eliminate any bacteria whereas they will not be able to survive without Arabinose present.

ENVIRONMENTAL SAFETY?

As mentioned above a kill switch will be available in the organism which will initiate cell death when the cell is in an environment that does not contain enough Arabinose.

2. Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues?

We have followed all the safety procedures during the isolation process from the cells we had and we successfully isolated the DNA without any problems that will raise safety issues.

3.Is there a local biosafety group, committee, or review board at your institution? If yes, what does your local biosafety group think about your project? If no, which specific biosafety rules or guidelines do you have to consider in your country?

In our university we have a biosafety and ethical research center which monitors the safety of our projects. http://www.ueam.metu.edu.tr The members of METU Biology department are also members of National Biosafety Coordinating Committee. We are able to consult to them whenever it is necessary. Also biosafety is a very important subject for METU Department of Biology, whereas the student members are very well trained in the subject. Also the projects that we decided to work on are presented to many people in the area in order to see if they are of any concern for the biosafety regulations. All the procedures that are used in the lab are also approved procedures that are used by these people mentioned above.

4. Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?

We believe using specific strains designed specifically for labwork will be safer. It is possible to genetically modify the organisms so that, they are not able to grow outside the lab environment. There probably is not a way to build some biobricks that can be modified in order to make them safer since their function will be the cause of safety issues.

Our Supporter: