Degredo PETronum!

OVERVIEW
The usage of polyethylene terephthalate-PET (as like plastic bottles or textiles) is one of the big problems of our era. Many plastic bottles are not reusable and are not safe for hot liquids or for use in dishwashers or microwaves. Some tougher, reusable plastic bottles may cause cancer. The main disadvantage of plastic bottles is that so many of them end up in landfills instead of being recycled. It takes thousands of years for plastic bottles to biodegrade, and the degrading process emits toxic chemicals into the air. We may alter the destiny of plastic in useful way.

OUR MISSION
Our project aims to clean enviroment with the degredation of PET to pyruvate by E.coli. With this project,while the environment will be cleaned from PET, the E.coli would add pyruvate to the cell cycle and use it as a new source. We divide our project into two parts. At the first part we found gene design for converting the PET into 2-hydroxymuconate semialdehyde in iGEM database. The scheme at the bottom explains the first part
PET (polyethylene terephthalate)
TPA(terephthalic acid)
DCD ((1R,2S)-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylic acid)
PCA (protocatechuic acid)
Catechol
2-hydroxymuconate semialdehyde

At the second part, we wanted to go further and researched the gene parts for convertion of 2-hydroxymuconate semialdehyde into pyruvate. This part will give us the chance of adding new parts to iGEM database. In this scheme, it can be observed in detail.
2-hydroxymuconate semialdehyde
2-oxopent-4-enoate
4-hydroxy-2-oxopentanoate
Pyruvate

REFERENCES
Hawthorne, K. (n.d). Advantages & Disadvantages of Plastic Bottles. Retrieved from
http://www.ehow.com/info_8242434_advantages-disadvantages-plastic-bottles.html

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Requirements

Please be sure to keep these links, your audience will want to find your:

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• All pages, images and files must be hosted on the 2014.igem.org server.
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Visit the Wiki How To page for a complete list of requirements, tips and other useful information.

Tips

We are currently working on providing teams with some easy to use design templates.
In the meantime you can also view other team wikis for inspiration! Here are some very good examples

• 2013 SDU Denmark
• 2013 SYSU China
• 2013 Shenxhen BGIG ATCG
• 2013 Colombia Unianades
• 2013 Lethbridge

For a full wiki list, you can visit iGEM 2013 web sites and iGEM 2012 web sites lists.

This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started:

• State your accomplishments! Tell people what you have achieved from the start.
• Be clear about what you are doing and what you plan to do.
• You have a global audience! Consider the different backgrounds that your users come from.
• Make sure information is easy to find; nothing should be more than 3 clicks away.
• Avoid using very small fonts and low contrast colors; information should be easy to read.
• Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the iGEM 2013 calendar
• Have lots of fun!