http://2014.igem.org/wiki/index.php?title=Team:Linkoping_Sweden/Project/Solution&feed=atom&action=historyTeam:Linkoping Sweden/Project/Solution - Revision history2024-03-28T10:23:49ZRevision history for this page on the wikiMediaWiki 1.16.5http://2014.igem.org/wiki/index.php?title=Team:Linkoping_Sweden/Project/Solution&diff=314938&oldid=prevPatriciag at 10:51, 17 October 20142014-10-17T10:51:49Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>To detect the FRET effect we will need an apparatus which can excite FITC and at the same time detect FRET between FITC and RFP.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>To detect the FRET effect we will need an apparatus which can excite FITC and at the same time detect FRET between FITC and RFP <ins class="diffchange diffchange-inline">(Fig. 1)</ins>.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Our aim is to build a very small fixed wavelength spectrophotometer with good performance to a relatively low cost.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Our aim is to build a very small fixed wavelength spectrophotometer with good performance to a relatively low cost.</p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>The excitation energy will come from a LED source and only wavelengths between 480-500 nm will pass a short band pass optical filter with a peak maximum at 488 nm. This overlaps very precisely with the excitation peak for FITC. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>The excitation energy will come from a LED source and only wavelengths between 480-500 nm will pass a short band pass optical filter with a peak maximum at 488 nm. This overlaps very precisely with the excitation peak for FITC. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>This light hits the sample cuvette and makes FRET occur between the complexes. The now red-shifted photons emitted from RFP will pass another filter, which only allows red light through, and hits a calibrated Si-photodiode detector. This will give an output that can be read by a microcontroller, in our case an Arduino.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>This light hits the sample cuvette and makes FRET occur between the complexes. The now red-shifted photons emitted from RFP will pass another filter, which only allows red light through, and hits a calibrated Si-photodiode detector. This will give an output that can be read by a microcontroller, in our case an Arduino <ins class="diffchange diffchange-inline">(Fig. 2)</ins>.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>If ara h 1 protein is available, the antibodies will bind the protein instead of the RFP-complex and thus give a reduction or extinction of FRET. This will be measured with the Si-detector as very little or no photons will pass the second optical filter. The signal will be reduced and a red LED lamp on the top of the detector will shine, indicating that peanut protein is present in the sample. If the sample does not contain any peanut protein a green LED will indicate this instead.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>If ara h 1 protein is available, the antibodies will bind the protein instead of the RFP-complex and thus give a reduction or extinction of FRET. This will be measured with the Si-detector as very little or no photons will pass the second optical filter. The signal will be reduced and a red LED lamp on the top of the detector will shine, indicating that peanut protein is present in the sample. If the sample does not contain any peanut protein a green LED will indicate this instead.</p></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p><del class="diffchange diffchange-inline">Fig </del>1. First prototype of our biosensor. Second generation under construction.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p><ins class="diffchange diffchange-inline">Figure </ins>1. First prototype of our biosensor. Second generation under construction.</p></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p><del class="diffchange diffchange-inline">Fig </del>2. Schematic presentation of our biosensor system. Photons from the LED source with wavelength ~488 will pass the first filter in order to reach the sample cuvette. This wavelength is the excitation peak for FITC. If FRET occurs only wavelengths above 550 nm (red) will pass the second filter and will be detected.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p><ins class="diffchange diffchange-inline">Figure </ins>2. Schematic presentation of our biosensor system. Photons from the LED source with wavelength ~488 will pass the first filter in order to reach the sample cuvette. This wavelength is the excitation peak for FITC. If FRET occurs only wavelengths above 550 nm (red) will pass the second filter and will be detected.</p></div></td></tr>
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</table>Patriciaghttp://2014.igem.org/wiki/index.php?title=Team:Linkoping_Sweden/Project/Solution&diff=311807&oldid=prevE.chamberlain at 09:24, 17 October 20142014-10-17T09:24:45Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>LiU iGEM’s vision is to develop a biosensor system for the private consumer. This system would detect the Ara h1 allergen and give an easily interpreted result, thus making it simple to analyze food and drink for potential peanut content. Being able to detect peanut protein in consumable goods would allow the allergic individual to avoid ingesting it and thereby completely evade an allergic reaction.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>LiU iGEM’s vision is to develop a biosensor system for the private consumer. This system would detect the Ara h1 allergen and give an easily interpreted result, thus making it simple to analyze food and drink for potential peanut content. Being able to detect peanut protein in consumable goods would allow the allergic individual to avoid ingesting it and thereby completely evade an allergic reaction.</p></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p>We believe that our device will be of great use for all peanut allergic individuals in everyday life but also for those who, for example, travel abroad and are unsure whether or not their food contains peanuts. Miscommunication or bad labeling is an extremely unnecessary reason to end up in a potentially life threatening situation. Therefore, by providing the individual with a chance to scan their food for peanuts, we will give them the security that they deserve.</p></ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Furthermore, this red light is in turn converted to a green signal meaning the food is safe to consume. However, if the full length Ara h1 protein exists in the solution it will bind to the antibodies with a higher affinity leading to a loss of the epitope – RFP complex and a deletion or severe reduction of FRET. Furthermore, when Ara h1 has bound to the antibodies, only the wavelength of FITC will be detected which will be characterized by a red light, meaning that the food is not safe from peanut protein content. If you want to read more about this go to <a href="https://2014.igem.org/Team:Linkoping_Sweden/Results">Results</a></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Furthermore, this red light is in turn converted to a green signal meaning the food is safe to consume. However, if the full length Ara h1 protein exists in the solution it will bind to the antibodies with a higher affinity leading to a loss of the epitope – RFP complex and a deletion or severe reduction of FRET. Furthermore, when Ara h1 has bound to the antibodies, only the wavelength of FITC will be detected which will be characterized by a red light, meaning that the food is not safe from peanut protein content. If you want to read more about this go to <a href="https://2014.igem.org/Team:Linkoping_Sweden/Results">Results</a></p></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><p>We believe that our device will be of great use for all peanut allergic individuals in everyday life but also for those who, for example, travel abroad and are unsure whether or not their food contains peanuts. Miscommunication or bad labeling is an extremely unnecessary reason to end up in a potentially life threatening situation. Therefore, by providing the individual with a chance to scan their food for peanuts, we will give them the security that they deserve.</p></del></div></td><td colspan="2"> </td></tr>
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</table>E.chamberlainhttp://2014.igem.org/wiki/index.php?title=Team:Linkoping_Sweden/Project/Solution&diff=286793&oldid=prevE.chamberlain at 20:13, 16 October 20142014-10-16T20:13:00Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>LiU iGEM’s vision is to develop a biosensor system for the private consumer. This system would detect the Ara <del class="diffchange diffchange-inline">h </del>allergen and give an easily <del class="diffchange diffchange-inline">interpretable </del>result, thus making it simple to analyze food and drink for potential peanut content. Being able to detect peanut protein in consumable goods would allow the allergic individual to avoid ingesting it and thereby completely evade an allergic reaction.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>LiU iGEM’s vision is to develop a biosensor system for the private consumer. This system would detect the Ara <ins class="diffchange diffchange-inline">h1 </ins>allergen and give an easily <ins class="diffchange diffchange-inline">interpreted </ins>result, thus making it simple to analyze food and drink for potential peanut content. Being able to detect peanut protein in consumable goods would allow the allergic individual to avoid ingesting it and thereby completely evade an allergic reaction.</p></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>By <del class="diffchange diffchange-inline">creating </del>a <del class="diffchange diffchange-inline">biobrick including </del>the sequence <del class="diffchange diffchange-inline">of </del>the Ara h1 epitope linked to a red fluorescent protein (RFP) we will be able to express the protein and thereby mix it with <del class="diffchange diffchange-inline">our ordered </del>solution of Ara h1 specific IgG antibodies. <del class="diffchange diffchange-inline"> </del>The epitope - RFP protein complex will then bind to the antibodies, which in turn are labeled with a green fluorescent probe. Hence, a phenomenon called <a href="https://2014.igem.org/Team:Linkoping_Sweden/Project/Biology">FRET</a> will occur when light is transferred from the green fluorescent probe to the red fluorescent protein by the use of a light source, thus creating an overlap in wavelength followed by detection in the form of a red light.</p> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>By <ins class="diffchange diffchange-inline">designing </ins>a <ins class="diffchange diffchange-inline">Biobrick which includes </ins>the sequence <ins class="diffchange diffchange-inline">for </ins>the Ara h1 epitope linked to a red fluorescent protein (RFP)<ins class="diffchange diffchange-inline">, </ins>we will be able to express the protein and thereby mix it with <ins class="diffchange diffchange-inline">a purchased </ins>solution of Ara h1 specific IgG antibodies. The epitope - RFP protein complex will then bind to the antibodies, which in turn are labeled with a green fluorescent probe<ins class="diffchange diffchange-inline">, Fluorescein isothiocyanate (FITC)</ins>. Hence, a phenomenon called <a href="https://2014.igem.org/Team:Linkoping_Sweden/Project/Biology">FRET</a> will occur when light is transferred from the green fluorescent probe to the red fluorescent protein by the use of a light source, thus creating an overlap in wavelength followed by detection in the form of a red light.</p> </div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p><del class="diffchange diffchange-inline">Further</del>, this red light is in turn converted to a green signal meaning the food is safe to consume. However, if the full length Ara h1 protein exists in the solution it will bind to the antibodies with a higher affinity leading to a loss of the epitope – RFP complex and a deletion of FRET. Furthermore, when Ara h1 has bound to the antibodies only the wavelength of FITC will be detected which will be characterized by a red light, meaning that the food is not safe from peanut protein content. If you want to read more about this go to <a href="https://2014.igem.org/Team:Linkoping_Sweden/Results">Results</a></p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p><ins class="diffchange diffchange-inline">Furthermore</ins>, this red light is in turn converted to a green signal meaning the food is safe to consume. However, if the full length Ara h1 protein exists in the solution it will bind to the antibodies with a higher affinity leading to a loss of the epitope – RFP complex and a deletion <ins class="diffchange diffchange-inline">or severe reduction </ins>of FRET. Furthermore, when Ara h1 has bound to the antibodies<ins class="diffchange diffchange-inline">, </ins>only the wavelength of FITC will be detected which will be characterized by a red light, meaning that the food is not safe from peanut protein content. If you want to read more about this go to <a href="https://2014.igem.org/Team:Linkoping_Sweden/Results">Results</a></p></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>We believe that our device will be of great use for all <del class="diffchange diffchange-inline">nut </del>allergic individuals in everyday life but also <del class="diffchange diffchange-inline">from </del>those who for example travel abroad and are unsure whether or not their food contains peanuts. Miscommunication or bad labeling is an extremely unnecessary reason to end up in a potentially life threatening situation. Therefore, by providing the individual a chance to scan their food for peanuts, we will give them the security that they deserve.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>We believe that our device will be of great use for all <ins class="diffchange diffchange-inline">peanut </ins>allergic individuals in everyday life but also <ins class="diffchange diffchange-inline">for </ins>those who<ins class="diffchange diffchange-inline">, </ins>for example<ins class="diffchange diffchange-inline">, </ins>travel abroad and are unsure whether or not their food contains peanuts. Miscommunication or bad labeling is an extremely unnecessary reason to end up in a potentially life threatening situation. Therefore, by providing the individual <ins class="diffchange diffchange-inline">with </ins>a chance to scan their food for peanuts, we will give them the security that they deserve.</p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>To detect the FRET effect we will need an apparatus which can excite FITC and at the same time detect FRET between FITC and RFP.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>To detect the FRET effect we will need an apparatus which can excite FITC and at the same time detect FRET between FITC and RFP.</p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>Our aim is to build a very small fixed wavelength spectrophotometer with good performance to a <del class="diffchange diffchange-inline">relative </del>low cost.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>Our aim is to build a very small fixed wavelength spectrophotometer with good performance to a <ins class="diffchange diffchange-inline">relatively </ins>low cost.</p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>The excitation energy will come from a LED source and only wavelengths between 480-500 nm will pass a short band pass optical filter with a peak maximum at 488 nm. This overlaps very precisely with the excitation peak for FITC. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>The excitation energy will come from a LED source and only wavelengths between 480-500 nm will pass a short band pass optical filter with a peak maximum at 488 nm. This overlaps very precisely with the excitation peak for FITC. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>This light hits the sample cuvette and makes FRET <del class="diffchange diffchange-inline">happen </del>between the complexes. The now red-shifted photons emitted from RFP will pass another filter, which only <del class="diffchange diffchange-inline">lets </del>red light through, and hits a calibrated Si-photodiode detector. This will give an output that can be read by a microcontroller, in our case an Arduino.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>This light hits the sample cuvette and makes FRET <ins class="diffchange diffchange-inline">occur </ins>between the complexes. The now red-shifted photons emitted from RFP will pass another filter, which only <ins class="diffchange diffchange-inline">allows </ins>red light through, and hits a calibrated Si-photodiode detector. This will give an output that can be read by a microcontroller, in our case an Arduino.</p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>If ara h 1 protein is available the antibodies will bind the protein instead of the RFP-complex and thus give a reduction or extinction of FRET. This will be measured with the Si-detector as very little or no photons will pass the second optical filter. The signal will be reduced and a red LED lamp on the top of the detector will shine, indicating that peanut protein is present in the sample. If the sample does not contain any peanut protein a green LED will indicate this instead.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>If ara h 1 protein is available<ins class="diffchange diffchange-inline">, </ins>the antibodies will bind the protein instead of the RFP-complex and thus give a reduction or extinction of FRET. This will be measured with the Si-detector as very little or no photons will pass the second optical filter. The signal will be reduced and a red LED lamp on the top of the detector will shine, indicating that peanut protein is present in the sample. If the sample does not contain any peanut protein a green LED will indicate this instead.</p></div></td></tr>
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</table>E.chamberlainhttp://2014.igem.org/wiki/index.php?title=Team:Linkoping_Sweden/Project/Solution&diff=273999&oldid=prevEmilo35 at 13:52, 16 October 20142014-10-16T13:52:42Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>This light hits the sample cuvette and makes FRET happen between the complexes. The now red-shifted photons emitted from RFP will pass another filter, which only lets red light through, and hits a calibrated Si-photodiode detector. This will give an output that can be read by a microcontroller, in our case an Arduino.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>This light hits the sample cuvette and makes FRET happen between the complexes. The now red-shifted photons emitted from RFP will pass another filter, which only lets red light through, and hits a calibrated Si-photodiode detector. This will give an output that can be read by a microcontroller, in our case an Arduino.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>If ara h 1 protein is available the antibodies will bind the protein instead of the RFP-complex and thus give a reduction or extinction of FRET. This will be measured with the Si-detector as very little or no photons will pass the second optical filter. The signal will be reduced and a red LED lamp on the top of the detector will shine, indicating that peanut protein is present in the sample. If the sample does not contain any peanut protein a green LED will indicate this instead.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>If ara h 1 protein is available the antibodies will bind the protein instead of the RFP-complex and thus give a reduction or extinction of FRET. This will be measured with the Si-detector as very little or no photons will pass the second optical filter. The signal will be reduced and a red LED lamp on the top of the detector will shine, indicating that peanut protein is present in the sample. If the sample does not contain any peanut protein a green LED will indicate this instead.</p></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><img src="https://static.igem.org/mediawiki/2014/7/7e/Linkoping_sweden_Detektor_med_hand.jpg" width="300px" height="auto" title="First prototype of our biosensor."></a></ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <img src="https://static.igem.org/mediawiki/2014/7/7e/Linkoping_sweden_Detektor_med_hand.jpg" width="649px" height="871px" title="First prototype of our biosensor."></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <p>Fig 1. First prototype of our biosensor. Second generation under construction.</p></ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p>Fig 1. First prototype of our biosensor. Second generation under construction.</p></ins></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <p>Fig <del class="diffchange diffchange-inline">1</del>. Schematic presentation of our biosensor system. Photons from the LED source with wavelength ~488 will pass the first filter in order to reach the sample cuvette. This wavelength is the excitation peak for FITC. If FRET occurs only wavelengths above 550 nm (red) will pass the second filter and will be detected.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <p>Fig <ins class="diffchange diffchange-inline">2</ins>. Schematic presentation of our biosensor system. Photons from the LED source with wavelength ~488 will pass the first filter in order to reach the sample cuvette. This wavelength is the excitation peak for FITC. If FRET occurs only wavelengths above 550 nm (red) will pass the second filter and will be detected.</p></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>Fig <del class="diffchange diffchange-inline">1</del>. Schematic presentation of our biosensor system. Photons from the LED source with wavelength ~488 will pass the first filter in order to reach the sample cuvette. This wavelength is the excitation peak for FITC. If FRET occurs only wavelengths above 550 nm (red) will pass the second filter and will be detected.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>Fig <ins class="diffchange diffchange-inline">2</ins>. Schematic presentation of our biosensor system. Photons from the LED source with wavelength ~488 will pass the first filter in order to reach the sample cuvette. This wavelength is the excitation peak for FITC. If FRET occurs only wavelengths above 550 nm (red) will pass the second filter and will be detected.</p></div></td></tr>
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</table>Emilo35http://2014.igem.org/wiki/index.php?title=Team:Linkoping_Sweden/Project/Solution&diff=269574&oldid=prevEmilo35 at 10:57, 16 October 20142014-10-16T10:57:40Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>LiU iGEM’s vision is to develop a biosensor system for the private consumer. This system would detect the Ara h allergen and give an easily interpretable result, thus making it simple to analyze food and drink for potential peanut content. Being able to detect peanut protein in consumable goods would allow the allergic individual to avoid ingesting it and thereby completely evade an allergic reaction.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>LiU iGEM’s vision is to develop a biosensor system for the private consumer. This system would detect the Ara h allergen and give an easily interpretable result, thus making it simple to analyze food and drink for potential peanut content. Being able to detect peanut protein in consumable goods would allow the allergic individual to avoid ingesting it and thereby completely evade an allergic reaction.</p></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><h1>Designing the biological components</h1></ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>By creating a biobrick including the sequence of the Ara h1 epitope linked to a red fluorescent protein (RFP) we will be able to express the protein and thereby mix it with our ordered solution of Ara h1 specific IgG antibodies. The epitope - RFP protein complex will then bind to the antibodies, which in turn are labeled with a green fluorescent probe. Hence, a phenomenon called <a href="https://2014.igem.org/Team:Linkoping_Sweden/Project/Biology">FRET</a> will occur when light is transferred from the green fluorescent probe to the red fluorescent protein by the use of a light source, thus creating an overlap in wavelength followed by detection in the form of a red light.</p> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>By creating a biobrick including the sequence of the Ara h1 epitope linked to a red fluorescent protein (RFP) we will be able to express the protein and thereby mix it with our ordered solution of Ara h1 specific IgG antibodies. The epitope - RFP protein complex will then bind to the antibodies, which in turn are labeled with a green fluorescent probe. Hence, a phenomenon called <a href="https://2014.igem.org/Team:Linkoping_Sweden/Project/Biology">FRET</a> will occur when light is transferred from the green fluorescent probe to the red fluorescent protein by the use of a light source, thus creating an overlap in wavelength followed by detection in the form of a red light.</p> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>Further, this red light is in turn converted to a green signal meaning the food is safe to consume. However, if the full length Ara h1 protein exists in the solution it will bind to the antibodies with a higher affinity leading to a loss of the epitope – RFP complex and a deletion of FRET. Furthermore, when Ara h1 has bound to the antibodies only the wavelength of FITC will be detected which will be characterized by a red light, meaning that the food is not safe from peanut protein content. If you want to read more about this go to <del class="diffchange diffchange-inline">results</del></p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>Further, this red light is in turn converted to a green signal meaning the food is safe to consume. However, if the full length Ara h1 protein exists in the solution it will bind to the antibodies with a higher affinity leading to a loss of the epitope – RFP complex and a deletion of FRET. Furthermore, when Ara h1 has bound to the antibodies only the wavelength of FITC will be detected which will be characterized by a red light, meaning that the food is not safe from peanut protein content. If you want to read more about this go to <ins class="diffchange diffchange-inline"><a href="https://2014.igem.org/Team:Linkoping_Sweden/Results">Results</a></ins></p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>We believe that our device will be of great use for all nut allergic individuals in everyday life but also from those who for example travel abroad and are unsure whether or not their food contains peanuts. Miscommunication or bad labeling is an extremely unnecessary reason to end up in a potentially life threatening situation. Therefore, by providing the individual a chance to scan their food for peanuts, we will give them the security that they deserve.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>We believe that our device will be of great use for all nut allergic individuals in everyday life but also from those who for example travel abroad and are unsure whether or not their food contains peanuts. Miscommunication or bad labeling is an extremely unnecessary reason to end up in a potentially life threatening situation. Therefore, by providing the individual a chance to scan their food for peanuts, we will give them the security that they deserve.</p></div></td></tr>
</table>Emilo35http://2014.igem.org/wiki/index.php?title=Team:Linkoping_Sweden/Project/Solution&diff=269545&oldid=prevPatriciag at 10:56, 16 October 20142014-10-16T10:56:07Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>LiU iGEM’s vision is to develop a biosensor system for the private consumer. This system would detect the Ara h allergen and give an easily interpretable result, thus making it simple to analyze food and drink for potential peanut content. Being able to detect peanut protein in consumable goods would allow the allergic individual to avoid ingesting it and thereby completely evade an allergic reaction.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>LiU iGEM’s vision is to develop a biosensor system for the private consumer. This system would detect the Ara h allergen and give an easily interpretable result, thus making it simple to analyze food and drink for potential peanut content. Being able to detect peanut protein in consumable goods would allow the allergic individual to avoid ingesting it and thereby completely evade an allergic reaction.</p></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p>By creating a biobrick including the sequence of the Ara h1 epitope linked to a red fluorescent protein (RFP) we will be able to express the protein and thereby mix it with our ordered solution of Ara h1 specific IgG antibodies. The epitope - RFP protein complex will then bind to the antibodies, which in turn are labeled with a green fluorescent probe. Hence, a phenomenon called <a href="https://2014.igem.org/Team:Linkoping_Sweden/Project/Biology">FRET</a> will occur when light is transferred from the green fluorescent probe to the red fluorescent protein by the use of a light source, thus creating an overlap in wavelength followed by detection in the form of a red light.</p> </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p>Further, this red light is in turn converted to a green signal meaning the food is safe to consume. However, if the full length Ara h1 protein exists in the solution it will bind to the antibodies with a higher affinity leading to a loss of the epitope – RFP complex and a deletion of FRET. Furthermore, when Ara h1 has bound to the antibodies only the wavelength of FITC will be detected which will be characterized by a red light, meaning that the food is not safe from peanut protein content. If you want to read more about this go to results</p></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>We believe that our device will be of great use for all nut allergic individuals in everyday life but also from those who for example travel abroad and are unsure whether or not their food contains peanuts. Miscommunication or bad labeling is an extremely unnecessary reason to end up in a potentially life threatening situation. Therefore, by providing the individual a chance to scan their food for peanuts, we will give them the security that they deserve.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>We believe that our device will be of great use for all nut allergic individuals in everyday life but also from those who for example travel abroad and are unsure whether or not their food contains peanuts. Miscommunication or bad labeling is an extremely unnecessary reason to end up in a potentially life threatening situation. Therefore, by providing the individual a chance to scan their food for peanuts, we will give them the security that they deserve.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"><p>By creating a biobrick including the sequence of the Ara h1 epitope linked to a red fluorescent protein (RFP) we will be able to express the protein and thereby mix it with our ordered solution of Ara h1 specific IgG antibodies. The epitope - RFP protein complex will then bind to the antibodies, which in turn are labeled with a green fluorescent probe. Hence, a phenomenon called <a href="https://2014.igem.org/Team:Linkoping_Sweden/Project/Biology">FRET</a> will occur when light is transferred from the green fluorescent probe to the red fluorescent protein by the use of a light source, thus creating an overlap in wavelength followed by detection in the form of a red light. Further, this red light is in turn converted to a green signal meaning the food is safe to consume. However, if the full length Ara h1 protein exists in the solution it will bind to the antibodies with a higher affinity leading to a loss of the epitope – RFP complex and a deletion of FRET. Furthermore, when Ara h1 has bound to the antibodies only the wavelength of FITC will be detected which will be characterized by a red light, meaning that the food is not safe from peanut protein content. If you want to read more about this go to results</p></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
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</table>Patriciaghttp://2014.igem.org/wiki/index.php?title=Team:Linkoping_Sweden/Project/Solution&diff=269319&oldid=prevAoborg at 10:44, 16 October 20142014-10-16T10:44:57Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>LiU iGEM’s vision is to develop a biosensor system for the private consumer. This system would detect the Ara h allergen and give an easily interpretable result, thus making it simple to analyze food and drink for potential peanut content. Being able to detect peanut protein in consumable goods would allow the allergic individual to avoid ingesting it and thereby completely evade an allergic reaction.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>LiU iGEM’s vision is to develop a biosensor system for the private consumer. This system would detect the Ara h allergen and give an easily interpretable result, thus making it simple to analyze food and drink for potential peanut content. Being able to detect peanut protein in consumable goods would allow the allergic individual to avoid ingesting it and thereby completely evade an allergic reaction.</p></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p>We believe that our device will be of great use for all nut allergic individuals in everyday life but also from those who for example travel abroad and are unsure whether or not their food contains peanuts. Miscommunication or bad labeling is an extremely unnecessary reason to end up in a potentially life threatening situation. Therefore, by providing the individual a chance to scan their food for peanuts, we will give them the security that they deserve.</p></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>By creating a biobrick including the sequence of the Ara h1 epitope linked to a red fluorescent protein (RFP) we will be able to express the protein and thereby mix it with our ordered solution of Ara h1 specific IgG antibodies. The epitope - RFP protein complex will then bind to the antibodies, which in turn are labeled with a green fluorescent probe. Hence, a phenomenon called <a href="https://2014.igem.org/Team:Linkoping_Sweden/Project/Biology">FRET</a> will occur when light is transferred from the green fluorescent probe to the red fluorescent protein by the use of a light source, thus creating an overlap in wavelength followed by detection in the form of a red light. Further, this red light is in turn converted to a green signal meaning the food is safe to consume. However, if the full length Ara h1 protein exists in the solution it will bind to the antibodies with a higher affinity leading to a loss of the epitope – RFP complex and a deletion of FRET. Furthermore, when Ara h1 has bound to the antibodies only the wavelength of FITC will be detected which will be characterized by a red light, meaning that the food is not safe from peanut protein content. If you want to read more about this go to results</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>By creating a biobrick including the sequence of the Ara h1 epitope linked to a red fluorescent protein (RFP) we will be able to express the protein and thereby mix it with our ordered solution of Ara h1 specific IgG antibodies. The epitope - RFP protein complex will then bind to the antibodies, which in turn are labeled with a green fluorescent probe. Hence, a phenomenon called <a href="https://2014.igem.org/Team:Linkoping_Sweden/Project/Biology">FRET</a> will occur when light is transferred from the green fluorescent probe to the red fluorescent protein by the use of a light source, thus creating an overlap in wavelength followed by detection in the form of a red light. Further, this red light is in turn converted to a green signal meaning the food is safe to consume. However, if the full length Ara h1 protein exists in the solution it will bind to the antibodies with a higher affinity leading to a loss of the epitope – RFP complex and a deletion of FRET. Furthermore, when Ara h1 has bound to the antibodies only the wavelength of FITC will be detected which will be characterized by a red light, meaning that the food is not safe from peanut protein content. If you want to read more about this go to results</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><p>We believe that our device will be of great use for all nut allergic individuals in everyday life but also from those who for example travel abroad and are unsure whether or not their food contains peanuts. Miscommunication or bad labeling is an extremely unnecessary reason to end up in a potentially life threatening situation. Therefore, by providing the individual a chance to scan their food for peanuts, we will give them the security that they deserve.</p></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div class="text-panel" style="margin-top:0px;padding-bottom:0px"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div class="text-panel" style="margin-top:0px;padding-bottom:0px"></div></td></tr>
</table>Aoborghttp://2014.igem.org/wiki/index.php?title=Team:Linkoping_Sweden/Project/Solution&diff=269238&oldid=prevTotomom at 10:40, 16 October 20142014-10-16T10:40:34Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div id="dialog" class="window" style="width:1463px;height:599px"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div id="dialog" class="window" style="width:1463px;height:599px"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/a/ac/Linkoping_sweden_detektor_mekanism.jpg" width="1463px" height="599px" title="Mechanism"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/a/ac/Linkoping_sweden_detektor_mekanism.jpg" width="1463px" height="599px" title="Mechanism"></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <p>Fig 1. <del class="diffchange diffchange-inline">Detector mechanism</del></p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <p>Fig 1. <ins class="diffchange diffchange-inline">Schematic presentation of our biosensor system. Photons from the LED source with wavelength ~488 will pass the first filter in order to reach the sample cuvette. This wavelength is the excitation peak for FITC. If FRET occurs only wavelengths above 550 nm (red) will pass the second filter and will be detected.</ins></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div id="mask"></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div id="mask"></div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>Fig 1. <del class="diffchange diffchange-inline">Detector mechanism (Click </del>to <del class="diffchange diffchange-inline">enlarge</del>).</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>Fig 1. <ins class="diffchange diffchange-inline">Schematic presentation of our biosensor system. Photons from the LED source with wavelength ~488 will pass the first filter in order </ins>to <ins class="diffchange diffchange-inline">reach the sample cuvette. This wavelength is the excitation peak for FITC. If FRET occurs only wavelengths above 550 nm (red</ins>) <ins class="diffchange diffchange-inline">will pass the second filter and will be detected</ins>.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td></tr>
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</table>Totomomhttp://2014.igem.org/wiki/index.php?title=Team:Linkoping_Sweden/Project/Solution&diff=257401&oldid=prevPatriciag at 22:51, 15 October 20142014-10-15T22:51:49Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>LiU iGEM’s vision is to develop a biosensor system for the private consumer. This system would detect the Ara h allergen and give an easily interpretable result, thus making it simple to analyze food and drink for potential peanut content. Being able to detect peanut protein in consumable goods would allow the allergic individual to avoid ingesting it and thereby completely evade an allergic reaction.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>LiU iGEM’s vision is to develop a biosensor system for the private consumer. This system would detect the Ara h allergen and give an easily interpretable result, thus making it simple to analyze food and drink for potential peanut content. Being able to detect peanut protein in consumable goods would allow the allergic individual to avoid ingesting it and thereby completely evade an allergic reaction.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>By creating a biobrick including the sequence of the Ara h1 epitope linked to a red fluorescent protein (RFP) we will be able to express the protein and thereby mix it with our ordered solution of Ara h1 specific IgG antibodies. The epitope - RFP protein complex will then bind to the antibodies, which in turn are labeled with a green fluorescent probe. Hence, a phenomenon called <a href="https://2014.igem.org/Team:Linkoping_Sweden/Project/Biology">FRET</a> will occur when light is transferred from the green fluorescent probe to the red fluorescent protein by the use of a light source, thus creating an overlap in wavelength followed by detection in the form of a red light. Further, this red light is in turn converted to a green signal meaning the food is safe to consume. However, if the full length Ara h1 protein exists in the solution it will bind to the antibodies with a higher affinity leading to a loss of the epitope – RFP complex and a deletion of FRET. Furthermore, when Ara h1 has bound to the antibodies only the wavelength of FITC will be detected which will be characterized by a red light, meaning that the food is not safe from peanut protein content.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>By creating a biobrick including the sequence of the Ara h1 epitope linked to a red fluorescent protein (RFP) we will be able to express the protein and thereby mix it with our ordered solution of Ara h1 specific IgG antibodies. The epitope - RFP protein complex will then bind to the antibodies, which in turn are labeled with a green fluorescent probe. Hence, a phenomenon called <a href="https://2014.igem.org/Team:Linkoping_Sweden/Project/Biology">FRET</a> will occur when light is transferred from the green fluorescent probe to the red fluorescent protein by the use of a light source, thus creating an overlap in wavelength followed by detection in the form of a red light. Further, this red light is in turn converted to a green signal meaning the food is safe to consume. However, if the full length Ara h1 protein exists in the solution it will bind to the antibodies with a higher affinity leading to a loss of the epitope – RFP complex and a deletion of FRET. Furthermore, when Ara h1 has bound to the antibodies only the wavelength of FITC will be detected which will be characterized by a red light, meaning that the food is not safe from peanut protein content. <ins class="diffchange diffchange-inline">If you want to read more about this go to results</ins></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>We believe that our device will be of great use for all nut allergic individuals in everyday life but also from those who for example travel abroad and are unsure whether or not their food contains peanuts. Miscommunication or bad labeling is an extremely unnecessary reason to end up in a potentially life threatening situation. Therefore, by providing the individual a chance to scan their food for peanuts, we will give them the security that they deserve.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>We believe that our device will be of great use for all nut allergic individuals in everyday life but also from those who for example travel abroad and are unsure whether or not their food contains peanuts. Miscommunication or bad labeling is an extremely unnecessary reason to end up in a potentially life threatening situation. Therefore, by providing the individual a chance to scan their food for peanuts, we will give them the security that they deserve.</p></div></td></tr>
</table>Patriciaghttp://2014.igem.org/wiki/index.php?title=Team:Linkoping_Sweden/Project/Solution&diff=255065&oldid=prevTotomom at 21:00, 15 October 20142014-10-15T21:00:30Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>The excitation energy will come from a LED source and only wavelengths between 480-500 nm will pass a short band pass optical filter with a peak maximum at 488 nm. This overlaps very precisely with the excitation peak for FITC. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>The excitation energy will come from a LED source and only wavelengths between 480-500 nm will pass a short band pass optical filter with a peak maximum at 488 nm. This overlaps very precisely with the excitation peak for FITC. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>This light hits the sample cuvette and makes FRET <del class="diffchange diffchange-inline">(länk till biology eller typ fret eller något) </del>happen between the complexes. The now red-shifted photons emitted from RFP will pass another filter, which only lets red light through, and hits a calibrated Si-photodiode detector. This will give an output that can be read by a microcontroller, in our case an Arduino.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>This light hits the sample cuvette and makes FRET happen between the complexes. The now red-shifted photons emitted from RFP will pass another filter, which only lets red light through, and hits a calibrated Si-photodiode detector. This will give an output that can be read by a microcontroller, in our case an Arduino.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>If ara h 1 protein is available the antibodies will bind the protein instead of the RFP-complex and thus give a reduction or extinction of FRET. This will be measured with the Si-detector as very little or no photons will pass the second optical filter. The signal will be reduced and a red LED lamp on the top of the detector will shine, indicating that peanut protein is present in the sample. If the sample does not contain any peanut protein a green LED will indicate this instead.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>If ara h 1 protein is available the antibodies will bind the protein instead of the RFP-complex and thus give a reduction or extinction of FRET. This will be measured with the Si-detector as very little or no photons will pass the second optical filter. The signal will be reduced and a red LED lamp on the top of the detector will shine, indicating that peanut protein is present in the sample. If the sample does not contain any peanut protein a green LED will indicate this instead.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></div></td></tr>
</table>Totomom