Team:Linkoping Sweden/Parts

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Parts

We have registered 5 different biobricks in the registry of parts and here you´ll get a small description of every biobrick.


<groupparts>iGEM014 Linkoping_Sweden</groupparts>

BBa_K1447002: Epitope 2 + E1010

This biobrick contains a designed sequence of Promotor T7 + RBS from the biobrick (BBa_K525998), a designed His-TEV sequence as well as a designed sequence of epitope 2 linked to RFP (E1010).

Figure X. Control-plate which lack bacterial growth.

Figure X. Control-plate which lack bacterial growth.

Figure X. Agar-plate of transformed bacterial cells with our Biobrick including RFP (E1010) linked to epitope 2.

Figure 1A. Agar-plate of transformed bacterial cells with our Biobrick including RFP (E1010) linked to epitope 2.(The front side of the plate).

Figure X. Agar-plate of transformed bacterial cells with our Biobrick including RFP (E1010) linked to epitope 2.

Figure 1B. Agar-plate of transformed bacterial cells with our Biobrick including RFP (E1010) linked to epitope 2. (Back side of the plate)

BBa_K1447005: Epitope 1-5 + J06504

This biobrick contains a designed sequence of Promotor T7 + RBS from the biobrick (BBa_K525998), a designed His-TEV sequence as well as a designed sequence of epitope 22, epitope 1, epitope 3, epitope 4 and epitope 17 linked to MCherry (J06504).

BBa_K1447003: Epitope 2 + J06504

This biobrick contains a designed sequence of Promotor T7 + RBS from the biobrick (BBa_K525998), a designed His-TEV sequence as well as a designed sequence of epitope 2 linked to MCherry (J06504).

Figure X. Control-plate which lack bacterial growth.

Figure X. Control-plate which lack bacterial growth.

Figure X. Agar-plate with transformed bacterial cells. One half of the plate includes transformed bacterial cells with our Biobrick containing MCherry linked to epitope 1-5 and the other half includes transformed bacterial cells with our Biobrick containing MCherry linked to epitope 2.

Figure X. Agar-plate with transformed bacterial cells. One half of the plate includes transformed bacterial cells with our Biobrick containing MCherry linked to epitope 1-5 and the other half includes transformed bacterial cells with our Biobrick containing MCherry linked to epitope 2.

Figure X. Agar-plate with transformed bacterial cells. One half of the plate includes transformed bacterial cells with our Biobrick containing MCherry linked to epitope 1-5 and the other half includes transformed bacterial cells with our Biobrick containing MCherry linked to epitope 2.

Figure X. Agar-plate with transformed bacterial cells. One half of the plate includes transformed bacterial cells with our Biobrick containing MCherry linked to epitope 1-5 and the other half includes transformed bacterial cells with our Biobrick containing MCherry linked to epitope 2.

BBa_K1447004: Epitope 1-5 + E1010

This biobrick contains a designed sequence of Promotor T7 + RBS from the biobrick (BBa_K525998), a designed His-TEV sequence as well as a designed sequence of epitope 22, epitope 1, epitope 3, epitope 4 and epitope 17 linked to RFP (E1010).

Figure X. Control-plate which lack bacterial growth.

Figure X. Control-plate which lack bacterial growth.

Figure X. Agar-plate with transformed bacterial cells. All 3 parts of the plate includes transformed bacterial cells with our Biobrick containing RFP linked to epitope 1-5. (Different colonies used).

Figure X. Agar-plate with transformed bacterial cells. All 3 parts of the plate includes transformed bacterial cells with our Biobrick containing RFP linked to epitope 1-5. (Different colonies used).

Figure X. Agar-plate with transformed bacterial cells. All 3 parts of the plate includes transformed bacterial cells with our Biobrick containing RFP linked to epitope 1-5. (Different colonies used).

Figure X. Agar-plate with transformed bacterial cells. All 3 parts of the plate includes transformed bacterial cells with our Biobrick containing RFP linked to epitope 1-5. (Different colonies used).

BBa_K1447007: His-TEV

This biobrick contains the sequence of Promotor T7 + RBS from the biobrick (BBa_K525998), as well as a designed His-TEV sequence.

Figure X. Control-plate which lack bacterial growth.

Figure X. Control-plate which lack bacterial growth.

Figure X. Agar-plate of transformed bacterial cells with our Biobrick including His-TEV.

Figure X. Agar-plate of transformed bacterial cells with our Biobrick including His-TEV.

Figure X. Agar-plate of transformed bacterial cells with our Biobrick including His-TEV.

Figure X. Agar-plate of transformed bacterial cells with our Biobrick including His-TEV.

Linköping University
581 83 Linköping, Sweden
liuigemgroup@gmail.com
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