Team:Linkoping Sweden/Parts

From 2014.igem.org

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<p style="font-weight:bold">BBa_K1447002: Epitope 2 + E1010</p>
<p style="font-weight:bold">BBa_K1447002: Epitope 2 + E1010</p>
<p>This biobrick contains a designed sequence of Promotor T7 + RBS from the biobrick (BBa_K525998), a designed His-TEV sequence as well as a designed sequence of epitope 2 linked to RFP (E1010).</p>
<p>This biobrick contains a designed sequence of Promotor T7 + RBS from the biobrick (BBa_K525998), a designed His-TEV sequence as well as a designed sequence of epitope 2 linked to RFP (E1010).</p>
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<img src="https://static.igem.org/mediawiki/2014/a/ae/Linkoping_sweden_Parts_IMG_3097%282%29.JPG" width="340px" height="auto" title="Control-plate"></a>
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    <div id="part1" class="window" style="width:800px;height:600px">
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        <img src="https://static.igem.org/mediawiki/2014/a/ae/Linkoping_sweden_Parts_IMG_3097%282%29.JPG" width="800px" height="600px" title="Control-plate">
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        <p>Figure X. Control-plate which lack bacterial growth.</p>
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<p>Figure X. Control-plate which lack bacterial growth.</p>
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<p>Figure 1B. Agar-plate of transformed bacterial cells with our Biobrick including RFP (E1010) linked to epitope 2. (Backside of the plate).</p>
<p>Figure 1B. Agar-plate of transformed bacterial cells with our Biobrick including RFP (E1010) linked to epitope 2. (Backside of the plate).</p>
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<p style="font-weight:bold">BBa_K1447005: Epitope 1-5 + J06504</p>
<p style="font-weight:bold">BBa_K1447005: Epitope 1-5 + J06504</p>
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<img src="https://static.igem.org/mediawiki/2014/a/ae/Linkoping_sweden_Parts_IMG_3097%282%29.JPG" width="340px" height="auto" title="Control-plate"></a>
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    <div id="part4" class="window" style="width:800px;height:600px">
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        <img src="https://static.igem.org/mediawiki/2014/a/ae/Linkoping_sweden_Parts_IMG_3097%282%29.JPG" width="800px" height="600px" title="Control-plate">
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        <p>Figure X. Control-plate which lack bacterial growth.</p>
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    </div> 
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<p>Figure X. Control-plate which lack bacterial growth.</p>
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<a href="#part5" name="modal">
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<p>Figure 2B. Agar-plate with transformed bacterial cells. One half of the plate includes transformed bacterial cells with our Biobrick containing MCherry linked to epitope 1-5 and the other half includes transformed bacterial cells with our Biobrick containing MCherry linked to epitope 2.(Backside of the plate)</p>
<p>Figure 2B. Agar-plate with transformed bacterial cells. One half of the plate includes transformed bacterial cells with our Biobrick containing MCherry linked to epitope 1-5 and the other half includes transformed bacterial cells with our Biobrick containing MCherry linked to epitope 2.(Backside of the plate)</p>
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<p>This biobrick contains a designed sequence of Promotor T7 + RBS from the biobrick (BBa_K525998), a designed His-TEV sequence as well as a designed sequence of epitope 22, epitope 1, epitope 3, epitope 4 and epitope 17 linked to RFP (E1010).</p>
<p>This biobrick contains a designed sequence of Promotor T7 + RBS from the biobrick (BBa_K525998), a designed His-TEV sequence as well as a designed sequence of epitope 22, epitope 1, epitope 3, epitope 4 and epitope 17 linked to RFP (E1010).</p>
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<img src="https://static.igem.org/mediawiki/2014/a/ae/Linkoping_sweden_Parts_IMG_3097%282%29.JPG" width="340px" height="auto" title="Control-plate"></a>
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    <div id="part7" class="window" style="width:800px;height:600px">
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        <img src="https://static.igem.org/mediawiki/2014/a/ae/Linkoping_sweden_Parts_IMG_3097%282%29.JPG" width="800px" height="600px" title="Control-plate">
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        <p>Figure X. Control-plate which lack bacterial growth.</p>
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    </div> 
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<p>Figure X. Control-plate which lack bacterial growth.</p>
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<div class="image-box" style="width:340px;padding-top:0px;float:left;margin:10px 5px 0px;">
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<p>Figure 3B. Agar-plate with transformed bacterial cells. All 3 parts of the plate includes different colonies of transformed bacterial cells with our Biobrick containing RFP linked to epitope 1-5. (Backside of the plate).</p>
<p>Figure 3B. Agar-plate with transformed bacterial cells. All 3 parts of the plate includes different colonies of transformed bacterial cells with our Biobrick containing RFP linked to epitope 1-5. (Backside of the plate).</p>
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<p>This biobrick contains the sequence of Promotor T7 + RBS from the biobrick (BBa_K525998), as well as a designed His-TEV sequence.</p>
<p>This biobrick contains the sequence of Promotor T7 + RBS from the biobrick (BBa_K525998), as well as a designed His-TEV sequence.</p>
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<div class="image-box" style="width:340px;padding-top:0px;float:left;margin:10px 5px 0px;">
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<img src="https://static.igem.org/mediawiki/2014/a/ae/Linkoping_sweden_Parts_IMG_3097%282%29.JPG" width="340px" height="auto" title="Control-plate"></a>
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<div id="boxes">
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    <div id="part10" class="window" style="width:800px;height:600px">
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        <img src="https://static.igem.org/mediawiki/2014/a/ae/Linkoping_sweden_Parts_IMG_3097%282%29.JPG" width="800px" height="600px" title="Control-plate">
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        <p>Figure X. Control-plate which lack bacterial growth.</p>
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    </div> 
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    <div id="mask"></div>
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<p>Figure X. Control-plate which lack bacterial growth.</p>
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<div class="image-box" style="width:340px;padding-top:0px;float:left;margin:10px 5px 0px;">
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<p>Figure 4B. Agar-plate of transformed bacterial cells with our Biobrick including His-TEV. (Frontside of the plate).</p>
<p>Figure 4B. Agar-plate of transformed bacterial cells with our Biobrick including His-TEV. (Frontside of the plate).</p>
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Revision as of 20:40, 17 October 2014

Parts

We have registered 5 different biobricks in the registry of parts and here you´ll get a small description of every biobrick.


<groupparts>iGEM014 Linkoping_Sweden</groupparts>

BBa_K1447002: Epitope 2 + E1010

This biobrick contains a designed sequence of Promotor T7 + RBS from the biobrick (BBa_K525998), a designed His-TEV sequence as well as a designed sequence of epitope 2 linked to RFP (E1010).

Figure 1A. Agar-plate of transformed bacterial cells with our Biobrick including RFP (E1010) linked to epitope 2. (Frontside of the plate).

Figure 1A. Agar-plate of transformed bacterial cells with our Biobrick including RFP (E1010) linked to epitope 2. (Frontside of the plate).

Figure 1B. Agar-plate of transformed bacterial cells with our Biobrick including RFP (E1010) linked to epitope 2. (Backside of the plate).

Figure 1B. Agar-plate of transformed bacterial cells with our Biobrick including RFP (E1010) linked to epitope 2. (Backside of the plate).

BBa_K1447005: Epitope 1-5 + J06504

This biobrick contains a designed sequence of Promotor T7 + RBS from the biobrick (BBa_K525998), a designed His-TEV sequence as well as a designed sequence of epitope 22, epitope 1, epitope 3, epitope 4 and epitope 17 linked to MCherry (J06504).

BBa_K1447003: Epitope 2 + J06504

This biobrick contains a designed sequence of Promotor T7 + RBS from the biobrick (BBa_K525998), a designed His-TEV sequence as well as a designed sequence of epitope 2 linked to MCherry (J06504).

Figure 2A. Agar-plate with transformed bacterial cells. One half of the plate includes transformed bacterial cells with our Biobrick containing MCherry linked to epitope 1-5 and the other half includes transformed bacterial cells with our Biobrick containing MCherry linked to epitope 2. (Frontside of the plate).

Figure 2A. Agar-plate with transformed bacterial cells. One half of the plate includes transformed bacterial cells with our Biobrick containing MCherry linked to epitope 1-5 and the other half includes transformed bacterial cells with our Biobrick containing MCherry linked to epitope 2. (Frontside of the plate).

Figure 2B. Agar-plate with transformed bacterial cells. One half of the plate includes transformed bacterial cells with our Biobrick containing MCherry linked to epitope 1-5 and the other half includes transformed bacterial cells with our Biobrick containing MCherry linked to epitope 2. (Backside of the plate)

Figure 2B. Agar-plate with transformed bacterial cells. One half of the plate includes transformed bacterial cells with our Biobrick containing MCherry linked to epitope 1-5 and the other half includes transformed bacterial cells with our Biobrick containing MCherry linked to epitope 2.(Backside of the plate)

BBa_K1447004: Epitope 1-5 + E1010

This biobrick contains a designed sequence of Promotor T7 + RBS from the biobrick (BBa_K525998), a designed His-TEV sequence as well as a designed sequence of epitope 22, epitope 1, epitope 3, epitope 4 and epitope 17 linked to RFP (E1010).

Figure 3A. Agar-plate with transformed bacterial cells. All 3 parts of the plate includes different colonies of transformed bacterial cells with our Biobrick containing RFP linked to epitope 1-5. (Frontside of the plate).

Figure 3A. Agar-plate with transformed bacterial cells. All 3 parts of the plate includes different colonies of transformed bacterial cells with our Biobrick containing RFP linked to epitope 1-5. (Frontside of the plate).

Figure 3B. Agar-plate with transformed bacterial cells. All 3 parts of the plate includes different colonies of transformed bacterial cells with our Biobrick containing RFP linked to epitope 1-5. (Backside of the plate).

Figure 3B. Agar-plate with transformed bacterial cells. All 3 parts of the plate includes different colonies of transformed bacterial cells with our Biobrick containing RFP linked to epitope 1-5. (Backside of the plate).

BBa_K1447007: His-TEV

This biobrick contains the sequence of Promotor T7 + RBS from the biobrick (BBa_K525998), as well as a designed His-TEV sequence.

Figure 4A. Agar-plate of transformed bacterial cells with our Biobrick including His-TEV. (Backside of the plate).

Figure 4A. Agar-plate of transformed bacterial cells with our Biobrick including His-TEV. (Backside of the plate)

Figure 4B. Agar-plate of transformed bacterial cells with our Biobrick including His-TEV. (Frontside of the plate).

Figure 4B. Agar-plate of transformed bacterial cells with our Biobrick including His-TEV. (Frontside of the plate).

Linköping University
581 83 Linköping, Sweden
liuigemgroup@gmail.com
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