Team:Linkoping Sweden/Parts

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<h1>Parts</h1>
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<p>We have registered five different Biobricks in the Official Registry of Parts and in the following section you will find a short description of every Biobrick registered.</p>
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<h1 >WELCOME TO iGEM 2014! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season!
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:Linkoping_Sweden/Parts&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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<groupparts>iGEM014 Linkoping_Sweden</groupparts>
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<a href="https://2014.igem.org/Team:Linkoping_Sweden"style="color:#000000">Home </a> </td>
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<a href="https://2014.igem.org/Team:Linkoping_Sweden/Team"style="color:#000000"> Team </a> </td>
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<a href="https://igem.org/Team.cgi?year=2014&team_name=Linkoping_Sweden"style="color:#000000"> Official Team Profile </a></td>
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<a href="https://2014.igem.org/Team:Linkoping_Sweden/Project"style="color:#000000"> Project</a></td>
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<a href="https://2014.igem.org/Team:Linkoping_Sweden/Parts"style="color:#000000"> Parts</a></td>
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<a href="https://2014.igem.org/Team:Linkoping_Sweden/Modeling"style="color:#000000"> Modeling</a></td>
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<a href="https://2014.igem.org/Team:Linkoping_Sweden/Notebook"style="color:#000000"> Notebook</a></td>
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<a href="https://2014.igem.org/Team:Linkoping_Sweden/Safety"style=" color:#000000"> Safety </a></td>
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<a href="https://2014.igem.org/Team:Linkoping_Sweden/Attributions"style="color:#000000"> Attributions </a></td>
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<td align ="center"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"></a> </td>
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<p style="font-weight:bold">BBa_K1447002: Epitope 2 + E1010</p>
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<p>This biobrick contains a designed sequence of Promotor T7 + RBS from the biobrick (BBa_K525998), a designed His-TEV sequence as well as a designed sequence of Ara h1 epitope 2 linked to RFP (E1010).</p>
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<img src="https://static.igem.org/mediawiki/2014/3/39/Linkoping_sweden_Parts_IMG_3107%282%29.JPG" width="340px" height="auto" title="Agar-plate"></a>
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        <img src="https://static.igem.org/mediawiki/2014/3/39/Linkoping_sweden_Parts_IMG_3107%282%29.JPG" width="800px" height="600px" title="Agar-plate">
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        <p>Figure 1A. Agar-plate of transformed bacterial cells with our Biobrick including RFP (E1010) linked to Ara h1 epitope 2. (Frontside of the plate).</p>
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    </div> 
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    <div id="mask"></div>
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<p>Figure 1A. Agar-plate of transformed bacterial cells with our Biobrick including RFP (E1010) linked to Ara h1 epitope 2. (Frontside of the plate).</p>
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<img src="https://static.igem.org/mediawiki/2014/9/9f/Linkoping_sweden_Parts_IMG_3108%282%29.JPG" width="340px" height="auto" title="Agar-plate"></a>
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<td > <h3>What information do I need to start putting my parts on the Registry? </h3></td>
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        <img src="https://static.igem.org/mediawiki/2014/9/9f/Linkoping_sweden_Parts_IMG_3108%282%29.JPG" width="800px" height="600px" title="Agar-plate">
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        <p>Figure 1B. Agar-plate of transformed bacterial cells with our Biobrick including RFP (E1010) linked to epitope 2. (Backside of the plate).</p>
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    </div> 
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An important aspect of the iGEM competition is the use and creation of standard  biological parts. Each team will make new parts during iGEM and will submit them to the <a href="http://partsregistry.org"> Registry of Standard Biological Parts</a>. The iGEM software provides an easy way to present the parts your team has created. The "groupparts" tag will generate a table with all of the parts that your team adds to your team sandbox.
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<p>Figure 1B. Agar-plate of transformed bacterial cells with our Biobrick including RFP (E1010) linked to Ara h1 epitope 2. (Backside of the plate).</p>
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<strong>Note that if you want to document a part you need to document it on the <a href="http://partsregistry.org Registry"> Registry</a>, not on your team wiki.</strong> Future teams and other users and are much more likely to find parts on the Registry than on your team wiki.
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Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without a need to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.
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<p style="font-weight:bold">BBa_K1447005: Ara h1 Epitopes 1-5 + J06504</p>
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<p>This biobrick contains a designed sequence of Promotor T7 + RBS from the biobrick (BBa_K525998), a designed His-TEV sequence as well as a designed sequence of Ara h1 epitope 22, epitope 1, epitope 3, epitope 4 and epitope 17 linked to MCherry (J06504).</p>
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<div style="display: inline-block;">
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<p style="font-weight:bold">BBa_K1447003: Epitope 2 + J06504</p>
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<p>This biobrick contains a designed sequence of Promotor T7 + RBS from the biobrick (BBa_K525998), a designed His-TEV sequence as well as a designed sequence of Ara h1 epitope 2 linked to MCherry (J06504).</p>
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<h3>When should you put parts into the Registry?</h3>
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<img src="https://static.igem.org/mediawiki/2014/f/ff/Linkoping_sweden_Parts_IMG_3109%282%29.JPG" width="340px" height="auto" title="Agar-plate"></a>
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        <img src="https://static.igem.org/mediawiki/2014/f/ff/Linkoping_sweden_Parts_IMG_3109%282%29.JPG" width="800px" height="600px" title="Agar-plate">
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        <p>Figure 2A. Agar-plate with transformed bacterial cells. One half of the plate includes transformed bacterial cells with our Biobrick containing MCherry linked to Ara h1 epitope 1-5 and the other half includes transformed bacterial cells with our Biobrick containing MCherry linked to Ara h1 epitope 2. (Frontside of the plate).</p>
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    </div> 
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    <div id="mask"></div>
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<p>Figure 2A. Agar-plate with transformed bacterial cells. One half of the plate includes transformed bacterial cells with our Biobrick containing MCherry linked to Ara h1 epitope 1-5 and the other half includes transformed bacterial cells with our Biobrick containing MCherry linked to Ara h1 epitope 2. (Frontside of the plate).</p>
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As soon as possible! We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better recall you will have of all details surrounding your parts. Remember you don't need to send us the DNA to create an entry for a part on the Registry. However, you must send us the sample/DNA before the Jamboree. Only parts for which you have sent us samples/DNA are eligible for awards and medal requirements.  
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<img src="https://static.igem.org/mediawiki/2014/c/c6/Linkoping_sweden_Parts_IMG_3110%282%29.JPG" width="340px" height="auto" title="Agar-plate"></a>
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        <img src="https://static.igem.org/mediawiki/2014/c/c6/Linkoping_sweden_Parts_IMG_3110%282%29.JPG" width="800px" height="600px" title="Agar-plate">
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        <p>Figure 2B. Agar-plate with transformed bacterial cells. One half of the plate includes transformed bacterial cells with our Biobrick containing MCherry linked to Ara h1 epitope 1-5 and the other half includes transformed bacterial cells with our Biobrick containing MCherry linked to Ara h1 epitope 2. (Backside of the plate)</p>
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    </div> 
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    <div id="mask"></div>
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<p>Figure 2B. Agar-plate with transformed bacterial cells. One half of the plate includes transformed bacterial cells with our Biobrick containing MCherry linked to epitope Ara h1 1-5 and the other half includes transformed bacterial cells with our Biobrick containing MCherry linked to Ara h1 epitope 2.(Backside of the plate)</p>
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<p style="font-weight:bold">BBa_K1447004: Epitope 1-5 + E1010</p>
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The information needed to initially create a part on the Registry is:
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<p>This biobrick contains a designed sequence of Promotor T7 + RBS from the biobrick (BBa_K525998), a designed His-TEV sequence as well as a designed sequence of Ara h1 epitope 22, epitope 1, epitope 3, epitope 4 and epitope 17 linked to RFP (E1010).</p>
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<ol>
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<li>Part Name</li>
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<li>Creator</li>
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<li>Sequence</li>
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<img src="https://static.igem.org/mediawiki/2014/f/ff/Linkoping_sweden_Parts_IMG_3112%282%29.JPG" width="340px" height="auto" title="Agar-plate"></a>
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<li>Short Description (60 characters on what the DNA does)</li>
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<li>Design considerations</li>
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        <img src="https://static.igem.org/mediawiki/2014/f/ff/Linkoping_sweden_Parts_IMG_3112%282%29.JPG" width="800px" height="600px" title="Agar-plate">
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        <p>Figure 3A. Agar-plate with transformed bacterial cells. All 3 parts of the plate includes different colonies of transformed bacterial cells with our Biobrick containing RFP linked to Ara h1 epitope 1-5. (Frontside of the plate).</p>
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    </div> 
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    <div id="mask"></div>
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</div>
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<p>Figure 3A. Agar-plate with transformed bacterial cells. All 3 parts of the plate includes different colonies of transformed bacterial cells with our Biobrick containing RFP linked to Ara h1 epitope 1-5. (Frontside of the plate).</p>
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. Check out part <a href="http://parts.igem.org/Part:BBa_K404003">BBa_K404003</a> for an excellent example of a highly characterized part.  
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<img src="https://static.igem.org/mediawiki/2014/f/f9/Linkoping_sweden_Parts_IMG_3113%282%29.JPG" width="340px" height="auto" title="Agar-plate"></a>
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    <div id="part9" class="window" style="width:800px;height:600px">
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        <img src="https://static.igem.org/mediawiki/2014/f/f9/Linkoping_sweden_Parts_IMG_3113%282%29.JPG" width="800px" height="600px" title="Agar-plate">
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        <p>Figure 3B. Agar-plate with transformed bacterial cells. All 3 parts of the plate includes different colonies of transformed bacterial cells with our Biobrick containing RFP linked to Ara h1 epitope 1-5. (Backside of the plate).</p>
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    </div> 
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    <div id="mask"></div>
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<p>Figure 3B. Agar-plate with transformed bacterial cells. All 3 parts of the plate includes different colonies of transformed bacterial cells with our Biobrick containing RFP linked to Ara h1 epitope 1-5. (Backside of the plate).</p>
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You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry"> Add a Part to the Registry</a> link.
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<p style="font-weight:bold">BBa_K1447007: His-TEV</p>
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<p>This biobrick contains the sequence of Promotor T7 + RBS from the biobrick (BBa_K525998), as well as a designed His-TEV sequence.</p>
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<img src="https://static.igem.org/mediawiki/2014/8/87/Linkoping_sweden_Parts_IMG_3114%282%29.JPG" width="340px" height="auto" title="Agar-plate"></a>
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        <img src="https://static.igem.org/mediawiki/2014/8/87/Linkoping_sweden_Parts_IMG_3114%282%29.JPG" width="800px" height="600px" title="Agar-plate">
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        <p>Figure 4A. Agar-plate of transformed bacterial cells with our Biobrick including His-TEV. (Backside of the plate).</p>
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    </div> 
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    <div id="mask"></div>
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</div>
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<p>Figure 4A. Agar-plate of transformed bacterial cells with our Biobrick including His-TEV. (Backside of the plate)</p>
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<tr><td colspan="3" > <h3> Parts Table</h3></td></tr>
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<img src="https://static.igem.org/mediawiki/2014/9/96/Linkoping_sweden_Parts_IMG_3115%282%29.JPG" width="340px" height="auto" title="Agar-plate"></a>
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    <div id="part12" class="window" style="width:800px;height:600px">
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        <img src="https://static.igem.org/mediawiki/2014/9/96/Linkoping_sweden_Parts_IMG_3115%282%29.JPG" width="800px" height="600px" title="Agar-plate">
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        <p>Figure 4B. Agar-plate of transformed bacterial cells with our Biobrick including His-TEV. (Frontside of the plate).</p>
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    </div> 
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    <div id="mask"></div>
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<p>Figure 4B. Agar-plate of transformed bacterial cells with our Biobrick including His-TEV. (Frontside of the plate).</p>
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Any parts your team has created will appear in this table below:</td></tr>
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<groupparts>iGEM013 Linkoping_Sweden</groupparts>
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Latest revision as of 23:53, 17 October 2014

Parts

We have registered five different Biobricks in the Official Registry of Parts and in the following section you will find a short description of every Biobrick registered.


<groupparts>iGEM014 Linkoping_Sweden</groupparts>

BBa_K1447002: Epitope 2 + E1010

This biobrick contains a designed sequence of Promotor T7 + RBS from the biobrick (BBa_K525998), a designed His-TEV sequence as well as a designed sequence of Ara h1 epitope 2 linked to RFP (E1010).

Figure 1A. Agar-plate of transformed bacterial cells with our Biobrick including RFP (E1010) linked to Ara h1 epitope 2. (Frontside of the plate).

Figure 1A. Agar-plate of transformed bacterial cells with our Biobrick including RFP (E1010) linked to Ara h1 epitope 2. (Frontside of the plate).

Figure 1B. Agar-plate of transformed bacterial cells with our Biobrick including RFP (E1010) linked to epitope 2. (Backside of the plate).

Figure 1B. Agar-plate of transformed bacterial cells with our Biobrick including RFP (E1010) linked to Ara h1 epitope 2. (Backside of the plate).

BBa_K1447005: Ara h1 Epitopes 1-5 + J06504

This biobrick contains a designed sequence of Promotor T7 + RBS from the biobrick (BBa_K525998), a designed His-TEV sequence as well as a designed sequence of Ara h1 epitope 22, epitope 1, epitope 3, epitope 4 and epitope 17 linked to MCherry (J06504).

BBa_K1447003: Epitope 2 + J06504

This biobrick contains a designed sequence of Promotor T7 + RBS from the biobrick (BBa_K525998), a designed His-TEV sequence as well as a designed sequence of Ara h1 epitope 2 linked to MCherry (J06504).

Figure 2A. Agar-plate with transformed bacterial cells. One half of the plate includes transformed bacterial cells with our Biobrick containing MCherry linked to Ara h1 epitope 1-5 and the other half includes transformed bacterial cells with our Biobrick containing MCherry linked to Ara h1 epitope 2. (Frontside of the plate).

Figure 2A. Agar-plate with transformed bacterial cells. One half of the plate includes transformed bacterial cells with our Biobrick containing MCherry linked to Ara h1 epitope 1-5 and the other half includes transformed bacterial cells with our Biobrick containing MCherry linked to Ara h1 epitope 2. (Frontside of the plate).

Figure 2B. Agar-plate with transformed bacterial cells. One half of the plate includes transformed bacterial cells with our Biobrick containing MCherry linked to Ara h1 epitope 1-5 and the other half includes transformed bacterial cells with our Biobrick containing MCherry linked to Ara h1 epitope 2. (Backside of the plate)

Figure 2B. Agar-plate with transformed bacterial cells. One half of the plate includes transformed bacterial cells with our Biobrick containing MCherry linked to epitope Ara h1 1-5 and the other half includes transformed bacterial cells with our Biobrick containing MCherry linked to Ara h1 epitope 2.(Backside of the plate)

BBa_K1447004: Epitope 1-5 + E1010

This biobrick contains a designed sequence of Promotor T7 + RBS from the biobrick (BBa_K525998), a designed His-TEV sequence as well as a designed sequence of Ara h1 epitope 22, epitope 1, epitope 3, epitope 4 and epitope 17 linked to RFP (E1010).

Figure 3A. Agar-plate with transformed bacterial cells. All 3 parts of the plate includes different colonies of transformed bacterial cells with our Biobrick containing RFP linked to Ara h1 epitope 1-5. (Frontside of the plate).

Figure 3A. Agar-plate with transformed bacterial cells. All 3 parts of the plate includes different colonies of transformed bacterial cells with our Biobrick containing RFP linked to Ara h1 epitope 1-5. (Frontside of the plate).

Figure 3B. Agar-plate with transformed bacterial cells. All 3 parts of the plate includes different colonies of transformed bacterial cells with our Biobrick containing RFP linked to Ara h1 epitope 1-5. (Backside of the plate).

Figure 3B. Agar-plate with transformed bacterial cells. All 3 parts of the plate includes different colonies of transformed bacterial cells with our Biobrick containing RFP linked to Ara h1 epitope 1-5. (Backside of the plate).

BBa_K1447007: His-TEV

This biobrick contains the sequence of Promotor T7 + RBS from the biobrick (BBa_K525998), as well as a designed His-TEV sequence.

Figure 4A. Agar-plate of transformed bacterial cells with our Biobrick including His-TEV. (Backside of the plate).

Figure 4A. Agar-plate of transformed bacterial cells with our Biobrick including His-TEV. (Backside of the plate)

Figure 4B. Agar-plate of transformed bacterial cells with our Biobrick including His-TEV. (Frontside of the plate).

Figure 4B. Agar-plate of transformed bacterial cells with our Biobrick including His-TEV. (Frontside of the plate).

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