Team:LZU-China/wetlab2

From 2014.igem.org

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<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" " http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">
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/*This is wetlab2*/
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<html xmlns=" http://www.w3.org/1999/xhtml">
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<head>
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<meta http-equiv="Content-Type" content="text/html; charset=gb2312" />
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<title>LZU-China 2014</title>
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<style type="text/css">
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body,ul,li,ol{list-style:none;padding:0px;margin:0px;font-size:12px;font-family:微软雅黑;}
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img{border:0px;}
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.picList{width:100%;float:left;}
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.picList .col{width:600px;height:397px;position:relative;overflow:hidden;float:left;margin:10px 0 0 10px;}
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.picList .col img,.col a{position:absolute;top:0px;left:0px;}
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.picList .col img{width:100%;}
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.picList .col a{display:block;width:580;height:377px;top:357px;background:rgba(0,0,0,0.7);color:white;font-style:normal;line-height:25px;padding:10px;opacity:0.8;text-decoration:none;}
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.msg{float:left;width:900px;position:relative;left:50%;margin:30px 0 0 -450px;font-size:14px;}
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.picList2{width:100%;float:left;}
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.picList2 .col{width:600px;height:397px;position:relative;overflow:hidden;float:left;margin:10px 0 0 10px;}
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.picList2 .col img,.col a{position:absolute;top:0px;left:0px;}
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.picList2 .col img{width:100%;}
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.picList2 .col a{display:block;width:580;height:377px;top:357px;background:rgba(0,0,0,0.7);color:white;font-style:normal;line-height:25px;padding:10px;opacity:0.8;text-decoration:none;}
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.msg{float:left;width:900px;position:relative;left:50%;margin:30px 0 0 -450px;font-size:14px;}
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.picList3{width:100%;float:left;}
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.picList3 .col{width:600px;height:397px;position:relative;overflow:hidden;float:left;margin:10px 0 0 10px;}
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.picList3 .col img,.col a{position:absolute;top:0px;left:0px;}
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.picList3 .col img{width:100%;}
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.picList3 .col a{
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display:block;
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width:580;
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height:377px;
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top:357px;
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background:rgba(0,0,0,0.7);
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color:white;
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font-style:normal;
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line-height:25px;
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padding:10px;
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opacity:0.8;
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text-decoration:none;
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}
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.msg{float:left;width:900px;position:relative;left:50%;margin:30px 0 0 -450px;font-size:14px;}
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.picList4{width:100%;float:left;}
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.picList4 .col{width:600px;height:397px;position:relative;overflow:hidden;float:left;margin:10px 0 0 10px;}
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.picList4 .col img,.col a{position:absolute;top:0px;left:0px;}
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.picList4 .col img{width:100%;}
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.picList4 .col a{display:block;width:580;height:377px;top:357px;background:rgba(0,0,0,0.7);color:white;font-style:normal;line-height:25px;padding:10px;opacity:0.8;text-decoration:none;}
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.msg{float:left;width:900px;position:relative;left:50%;margin:30px 0 0 -450px;font-size:14px;}
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.picList5{width:100%;float:left;}
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.picList5 .col{width:600px;height:397px;position:relative;overflow:hidden;float:left;margin:10px 0 0 10px;}
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.picList5 .col img,.col a{position:absolute;top:0px;left:0px;}
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.picList5 .col img{width:100%;}
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.picList5 .col a{display:block;width:580;height:377px;top:357px;background:rgba(0,0,0,0.7);color:white;font-style:normal;line-height:25px;padding:10px;opacity:0.8;text-decoration:none;}
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.msg{float:left;width:900px;position:relative;left:50%;margin:30px 0 0 -450px;font-size:14px;}
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.picList6{width:100%;float:left;}
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-
.picList6 .col{width:600px;height:397px;position:relative;overflow:hidden;float:left;margin:10px 0 0 10px;}
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.picList6 .col img,.col a{position:absolute;top:0px;left:0px;}
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.picList6 .col img{width:100%;}
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-
.picList6 .col a{display:block;width:580;height:377px;top:357px;background:rgba(0,0,0,0.7);color:white;font-style:normal;line-height:25px;padding:10px;opacity:0.8;text-decoration:none;}
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-
.msg{float:left;width:900px;position:relative;left:50%;margin:30px 0 0 -450px;font-size:14px;}
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+
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.picList7{width:100%;float:left;}
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-
.picList7 .col{width:600px;height:397px;position:relative;overflow:hidden;float:left;margin:10px 0 0 10px;}
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.picList7 .col img,.col a{position:absolute;top:0px;left:0px;}
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.picList7 .col img{width:100%;}
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.picList7 .col a{display:block;width:580;height:377px;top:357px;background:rgba(0,0,0,0.7);color:white;font-style:normal;line-height:25px;padding:10px;opacity:0.8;text-decoration:none;}
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.msg{float:left;width:900px;position:relative;left:50%;margin:30px 0 0 -450px;font-size:14px;}
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.picList8{width:100%;float:left;}
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-
.picList8 .col{width:600px;height:397px;position:relative;overflow:hidden;float:left;margin:10px 0 0 10px;}
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-
.picList8 .col img,.col a{position:absolute;top:0px;left:0px;}
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-
.picList8 .col img{width:100%;}
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-
.picList8 .col a{display:block;width:580;height:377px;top:357px;background:rgba(0,0,0,0.7);color:white;font-style:normal;line-height:25px;padding:10px;opacity:0.8;text-decoration:none;}
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.msg{float:left;width:900px;position:relative;left:50%;margin:30px 0 0 -450px;font-size:14px;}
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+
-
*{margin:0px;padding:0px;}
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-
body{background:#b1b1b1;margin:0px;padding:0px;font-size:14px;color:#000;}
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-
b#p1, b#p2, b#p3, b#p4, b#p5 {display:none;}
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-
#accordian {padding:0; margin:20px auto; list-style:none; width:730px; height:300px; overflow:hidden; position:relative; border:1px solid #682;}
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#accordian li {width:50px; height:300px; float:left;
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-moz-transition: 0.7s;
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-ms-transition: 0.7s;
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-o-transition: 0.7s;
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-webkit-transition: 0.7s;
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transition: 0.7s;
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}
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#accordian div {width:530px; height:300px; position:relative;}
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#accordian div span {display:block; background:rgba(0,0,0,.3); width:460px; height:30px; position:absolute; bottom:300px; right:0; padding:10px; color:#fff; font:normal 13px/30px 'georgia', serif; border-top:1px solid #000;
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}
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#accordian div span a {color:#fff;}
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#accordian li div > a {display:block; width:49px; height:300px; float:left; position:relative; color:#fff; text-decoration:none;box-shadow:-10px 0 10px -10px rgba(0,0,0,0.6);}
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#accordian li div > a b {display:block; width:280px; padding-left:20px; height:50px; position:absolute; left:50px; bottom:0; font:bold 24px/50px 'georgia', serif; text-shadow:0 0 2px rgba(0, 0, 0, 0.6);
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transform: rotate(-90deg);
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transform-origin:bottom left;
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-ms-transform: rotate(-90deg);
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-ms-transform-origin:bottom left;
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-webkit-transform: rotate(-90deg);
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-webkit-transform-origin:bottom left;
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-moz-transform: rotate(-90deg);
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-moz-transform-origin:bottom left;
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-o-transform: rotate(-90deg);
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-o-transform-origin:bottom left;
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}
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#accordian li div > a b {*left:0;}
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#accordian li img {display:block; float:left;}
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#accordian li.p1 {width:530px;}
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#accordian li.p1 div span {bottom:0;}
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#accordian li a.a1 {background:#b33866; border-left:1px solid #682;}
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#accordian li a.a2 {background:#8d4984; border-left:1px solid #9b5;}
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#accordian li a.a3 {background:#6559a1; border-left:1px solid #ce5;}
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#accordian li a.a4 {background:#3c6cc0; border-left:1px solid #ef7;}
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#accordian li a.a5 {background:#187adb; border-left:1px solid #28b;}
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#p1:target ~ #accordian li {width:50px;}
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#p1:target ~ #accordian li div span {bottom:300px;}
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#p1:target ~ #accordian li.p1 {width:530px;}
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#p1:target ~ #accordian li.p1 div span {bottom:0;}
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#p2:target ~ #accordian li {width:50px;}
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#p2:target ~ #accordian li div span {bottom:300px;}
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#p2:target ~ #accordian li.p2 {width:530px;}
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#p2:target ~ #accordian li.p2 div span {bottom:0;}
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#p3:target ~ #accordian li {width:50px;}
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#p3:target ~ #accordian li div span {bottom:300px;}
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#p3:target ~ #accordian li.p3 {width:530px;}
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#p3:target ~ #accordian li.p3 div span {bottom:0;}
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#p4:target ~ #accordian li {width:50px;}
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#p4:target ~ #accordian li div span {bottom:300px;}
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#p4:target ~ #accordian li.p4 {width:530px;}
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#p4:target ~ #accordian li.p4 div span {bottom:0;}
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#p5:target ~ #accordian li {width:50px;}
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#p5:target ~ #accordian li div span {bottom:300px;}
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#p5:target ~ #accordian li.p5 {width:530px;}
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#p5:target ~ #accordian li.p5 div span {bottom:0;}
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#contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .visualClear {display: none;} /*-- hides default wiki settings --*/
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.firstHeading {
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width: 975px;
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margin: 0px auto;
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padding-top: 100px;
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margin-bottom: 20px;
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font-family: Georgia, Times, "Times New Roman", serif;
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}
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h1, h2, h3, h4, h5 { font-family: Georgia, Times, "Times New Roman", serif;}
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#top-section { /*-- styling for default menu bar (edit, page, history, etc.) --*/
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background-color: #383838;
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border: 0 none;
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height: 14px;
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z-index: 100;
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top: 0;
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position: fixed;
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width: 975px;
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left: 50%;
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margin-left: -487px;
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}
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#top-section-bar { /*-- styling full width bar which hides behind default menu bar (edit, page, history, etc.) --*/
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background-color: #383838;
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height: 14px;
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display: block;
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z-index: 10;
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position: fixed;
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width: 100%;
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top: 0;
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}
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#menubar a:link, #menubar a:active, #menubar a:visited, #menubar a:hover, #menubar:hover { /*-- styling for default menu bar links (edit, page, history, etc.) --*/
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color: #727272;
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text-decoration: none;
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background-color: transparent;
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}
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body {
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background-color: #fff;
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}
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#globalWrapper, #content { /*-- changes default wiki settings --*/
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width: 100%;
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height: 100%;
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border: 0px;
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background-color: transparent;
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margin: 0px;
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padding: 0px;
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}
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html, body, .wrapper { /*-- changes default wiki settings --*/
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width: 100%;
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height: 100%;
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background-color: transparent;
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}
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#contentcontainer { /*-- creates container for all content on page --*/
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font-family: Arial, Helvetica, sans-serif;
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font-weight: normal;
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font-size: 14px;
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color: #414141;
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width: 960px;
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margin-left: auto;
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margin-right: auto;
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background-color: #transparent;
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margin-top: 0px;
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}
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.sidemenu, .sidemenu li {
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list-style-type: none;
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list-style-image: none;
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font-family: verdana;
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text-decoration: none;
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color:#000;
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font-size: 14px;
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}
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.sidemenu li {display: block;}
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.sidemenu a {
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text-decoration:none;
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color: #000;
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display: block;
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width: 180px;
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height: 100%;
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padding: 3px 5px;
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transition: .25s ease-in-out;
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-moz-transition: .25s ease-in-out;
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-webkit-transition: .25s ease-in-out;
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}
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.sidemenu a.greyout { color: #909090; }
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.sidemenu a:hover {
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color:#666;
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background-color: #d9f5aa;
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}
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.sub-sidemenu {font-size: 10px; margin-bottom: 20px; color: #666; display: none;}
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.sub-sidemenu a {color: #666;}
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div.underconst
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{
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padding:10px;
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border-radius:5px;
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background-color:pink;
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text-align:center;
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}
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a.anchor{display: block; position: relative; top: -80px; visibility: hidden;}
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+
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.contentpara {margin-bottom: 30px;}
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span.email{font-family: monospace; font-weight: normal;}
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+
-
fieldset {border: 1px solid #337f53;}
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+
-
.greyout {color: #A0A0A0;}
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-
.highlightme {background-color: #FFFF00;}
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-
 
+
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#alertContainer {border: 2px solid red; margin-bottom: 10px; width: 936px;}
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#annContainer {margin-left: 8px;}
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#newsContainer {border: 1px solid #ccc;}
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.newsTitle {
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display: block;
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color: #414141;
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font-size: 25px; 
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        font-family: Georgia, Times, "Times New Roman", serif;
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padding: 10px 15px 5px 10px;
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border-bottom: 1px solid #ccc;
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margin-bottom: 0px;
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}
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-
.newsItem {
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border-bottom: 1px solid #ccc;
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display: block;
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padding: 5px 15px 0px 10px;
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margin-bottom: 0px;
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}
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.newsItem h3 {
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width: auto;
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display: inline;
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font-size: 14px;
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font-family: Arial, Helvetica, sans-serif;
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padding: 0px;
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margin: 0px;
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}
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.newsItem img {
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float: right;
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clear: right;
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width: 80px;
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padding-left: 15px;
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display: inline-block;
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}
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-
.newsItem .newsDate {
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font-style: italic;
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font-size: 14px;
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-
display: inline-block;
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-
color: #999;
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float: right;
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padding: 0px 0px 10px 15px;
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}
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.newsItem p, .newsItem ul, .newsItem li {
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padding: 0px;
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margin-left: 20px;
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font-size: 12px;
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line-height: 1.2;
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}
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+
-
.newsItem p {margin-top: 10px;}
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-
.annItem {
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-
border: 2px solid #49b677;
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-
display: block;
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-
padding: 0px 15px 10px 10px;
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margin-bottom: 18px;
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position: relative;
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height: 160px;
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vertical-align: middle;
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}
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.annItem .annCentered {
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display: table-cell;
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vertical-align: middle;
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height: 160px;
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}
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+
-
.annCentered h3 {
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padding: 0px;
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display: block;
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-
margin-left: auto;
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margin-right: auto;
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-
text-align: center;
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-
}
+
-
 
+
-
.annItem ul, .annItem li {padding: 0px; margin: 0px;}
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-
.annItem li {list-style: none; margin: 5px}
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@charset "utf-8";
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-
body,h1,h2,h3,h4,h5,h6,hr,p,blockquote,dl,dt,dd,ul,ol,li,pre,form,fieldset,legend,button,input,textarea,th,td,figure{
+
-
margin:0;
+
-
padding:0;
+
-
font-size: 14px;
+
-
text-align: left;
+
-
}
+
-
html{background-color:#e9ecec}
+
-
table{table-layout:fixed;border-collapse:collapse}
+
-
section,article,aside,header,footer,nav,dialog,figure{display:block}
+
-
.clearfix:after{content:"\20";display:block;height:0;line-height:0;font-size:0;clear:both}
+
-
body,button,input,select,textarea,td,th{color:#333;font:12px/1.5 tahoma,arial,\5b8b\4f53}
+
-
h1,h2,h3,h4,h5,h6{font-size:100%}
+
-
address,cite,dfn,em,var{font-style:normal}
+
-
code,kbd,pre,samp{font-family:courier new,courier,monospace}
+
-
small{font-size:12px}ul,ol{list-style:none}a{text-decoration:none;color:#06C}
+
-
a:hover{text-decoration:underline;color:#06C}
+
-
sup{vertical-align:text-top}
+
-
sub{vertical-align:text-bottom}
+
-
legend{color:#000}
+
-
fieldset,img{border:0 none}
+
-
button,input,select,textarea{font-size:100%}
+
-
table{
+
-
border-collapse:collapse;
+
-
border-spacing:0;
+
-
text-align: center;
+
-
}
+
-
input:focus{outline:none}
+
-
.hd-main{height:60px;background:#fff;z-index:15;border-bottom:#b3b3b3 1px solid;box-shadow:0 0 5px #333}
+
-
.f-icon{background-image:url('http://www.codefans.net/jscss/demoimg/201403//frame-icon.png');background-repeat:no-repeat}
+
-
.hd-main{padding:0 5px;height:49px;min-width:800px;background:#252525;border-bottom:0}
+
-
.hd-main .has-pulldown{cursor:pointer;position:relative;*z-index:10000}
+
-
.hd-main .pulldown{position:absolute;cursor:default;display:none;top:30px;left:0}
+
-
.hd-main .pulldown .arrow{*margin-bottom:-1px;_margin-bottom:0;height:0;width:0;font-size:0;line-height:0;border-width:7px;border-style:solid;border-color:transparent transparent rgba(255, 255, 255,0.8) transparent;_filter:chroma(color=tomato);_border-color:tomato tomato rgba(255, 255, 255,0.8) tomato;position:relative;display:block;left:20px;z-index:7}
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-
.hd-main .pulldown .content{background:rgba(255, 255, 255,0.8);color:#333;text-align:left;border-radius:3px;border:rgb(175,175,175) 1px solid;border-width:0 1px 1px 1px;box-shadow:0 2px 4px rgba(0,0,0,.2);position:relative;z-index:6}
+
-
.hd-main .pulldown-canvas{position:absolute;top:0;left:0;width:100%;height:100%;opacity:0;filter:alpha(opacity=0)}
+
-
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      <td width="1201" height="265" rowspan="2"><p><img src="https://static.igem.org/mediawiki/2014/thumb/9/99/9.notebook.top.jpg/800px-9.notebook.top.jpg" width="1366" height="270" /></p></td>
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      <td width="100%" height="199" bgcolor="#187ADB">&nbsp;</td>
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      <td><p>&nbsp;</p>
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        <p>&nbsp;</p>
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        <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&quot;Rome was not built in a day.&quot; No matter which group we belong to, we took our work for almost a whole year. What did we do on earth? This notebook will show you in detail.</p>
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        <p>&nbsp;</p>
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        <p>&nbsp;</p>
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        <div><a href="https://2014.igem.org/Team:LZU-China/Notebook"><img src="https://static.igem.org/mediawiki/2014/8/8f/Z.wabb%281%29.png" width="214" height="99" /></a> <a href="https://2014.igem.org/Team:LZU-China/NotebookB"><img src="https://static.igem.org/mediawiki/2014/e/e6/Z.wabb%282%29.png" width="215" height="99" /></a><a href="https://2014.igem.org/Team:LZU-China/NotebookC"><img src="https://static.igem.org/mediawiki/2014/0/0e/Z.wabb%283%29.png" width="212" height="99" /></a></div>
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      <p>&nbsp;</p></td>
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          <td background="https://static.igem.org/mediawiki/2014/a/a6/Huils.jpg" >&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;THE NOTEBOOK OF WET LAB GROUP A</td>
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      <td background="https://static.igem.org/mediawiki/2014/a/a6/Huils.jpg" ><table width="100%" border="0">
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          <td background="https://static.igem.org/mediawiki/2014/a/a6/Huils.jpg"  width="30%">&nbsp;</td>
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          <td background="https://static.igem.org/mediawiki/2014/a/a6/Huils.jpg"  width="57%"><p>&nbsp;</p>
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            <p>&nbsp;</p>
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            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2014.5.16-5.23<br />
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              &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;The pre-experience of the growth of three  strains(<em>Shewanella onedensis,</em> <em>Pseudomonas aeruginosa </em>and LZU-3, which is separated in Li&rsquo;s  lab )in different PNP concentration and  Cr(VI) concentration.</p>
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            <p>&nbsp;</p>
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            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<img src="https://static.igem.org/mediawiki/2014/f/f5/Z.da1.png" width="700" height="173" /></p>
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            <div><img src="file:///E|/[iGEM]/wiki/2014logomin.png" width="358" height="40" /></div>
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            <p>&nbsp;</p>
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            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<img src="file:///E|/https://static.igem.org/mediawiki/2014/e/e0/Partpic2.png" width="301" height="251" /></p>
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            <div><img src="file:///E|/[iGEM]/wiki/partpic2.png" width="301" height="251" /></div>
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            <p>&nbsp;</p>
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            <p>&nbsp;</p>
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            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2014.5.24-5.31<br />
+
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              &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Measurement for three strains&rsquo;(<em>Shewanella onedensis,</em> <em>Pseudomonas aeruginosa </em>and LZU-3, which is separated in Li&rsquo;s  lab ) Cr(VI) reduction activity.</p>
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            <div><img src="file:///E|/[iGEM]/wiki/dblbluebar.jpg" alt="" width="1365" height="463" /></div>
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            <p>&nbsp;</p>
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            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<img src="https://static.igem.org/mediawiki/2014/thumb/d/dc/Z.a2.png/463px-Z.a2.png" width="467" height="605" /></p>
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<p>&nbsp;</p>
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            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2014.6.1-2014.6.8<br />
+
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              &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1.Another measurement for three strains&rsquo;(as above  said) Cr(VI) reduction activity.</p>
+
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            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<img src="https://static.igem.org/mediawiki/2014/a/a6/Z.a3.png" width="569" height="482" /></p>
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            <p>&nbsp;</p>
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            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2.Construction of the phylogeny tree containing the LZU-3 </p>
+
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            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<img src="https://static.igem.org/mediawiki/2014/5/52/Z.4.png" width="654" height="503" /></p>
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            <p>&nbsp;</p>
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            <p>&nbsp;</p>
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            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2014.6.8-2014.7.24<br />
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;The PCR cycling for BBa_I723020 and BBa_I723133</p>
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            <p>&nbsp;</p>
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            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1. The primers(synthesized by BGI,shenzhen)</p>
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            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1.1 Promoter of BBa_I723133 <br />
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              The forward primer (5&rsquo;  TATACTAGTCTGGGGCGAGAGGCGACGAC 3&rsquo;) contained a SpeI cutsite <br />
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              The reverse primer (5&rsquo;  GGGGAATTCTAGAATGTGGGCTGCTTGGTG 3&rsquo;) contained a EcoRI and XbaI cutsite. <br />
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              2313bp</p>
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            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1.2 BBa_I723020<br />
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              The forward primer  (5&rsquo;GGTGCTGCAGACTAGTATTGAAGGGTCACCACTATTTTTATTTTA 3&rsquo;) containes SpeI and PstI  cutsites <br />
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              the reverse primer (5&rsquo;  ATGAATTCTCTAGACCTGCTGGAGGGCGTGAAC 3&rsquo;) containes EcoRI and XbaI cutsites. <br />
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              340bp</p>
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            <p>&nbsp;</p>
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            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2.The  PCR system</p>
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            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;0.2&mu;L ExTaq.<br />
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              &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1&mu;L bacterial liquid<br />
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              &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1&mu;L dNTP<br />
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              &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1&mu;L forward primer<br />
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              &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1&mu;L reverse primer<br />
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              &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2.5&mu;L 10x Buffer<br />
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              &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;18.5&mu;L ddH2O</p>
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<p>&nbsp;</p>
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            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2014.7.31-8.1<br />
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              &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Pre-experience for the function of BBa_K381001.<br />
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              &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;BBa_K381001 transformation to E.coli DH5 alpha<br />
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              &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;There are two groups to test the green fluorescence under microscope:<br />
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              &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(These groups' total sizes are all 20&mu;L, had been shaken for 2hours before observation,400x)<br />
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              &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;a.bacterial liquid with 10mM PNP concentration.<br />
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              &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;b.bacterial liquid with 20mM PNP concentration.<br />
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            </p>
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            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<img src="https://static.igem.org/mediawiki/2014/2/2d/Z.5.png" width="704" height="307" /></p>
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            <p>&nbsp;</p>
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            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2014.8.2-2014.8.6<br />
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              Pre-experience for the function of BBa_K381001.<br />
+
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              BBa_K381001 transformation to E.coli DH5 alpha<br />
+
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              There are four groups to test the green fluorescence under microscope:<br />
+
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              (These groups' total sizes are all 20&mu;L, had been shaken for 2hours before observation,1000x)<br />
+
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              &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;a.bacterial liquid with 10mM PNP concentration.b.<br />
+
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              &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;b.bacterial liquid with ddH2O<br />
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              &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;c.bacterial liquid with 10mM KNO3<br />
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              &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;d.bacterial liquid with 10mM KCl.<br />
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            </p>
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            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<img src="https://static.igem.org/mediawiki/2014/4/41/Z.a6.png" width="455" height="340" /></p>
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            <p>&nbsp;</p>
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            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2014.8.25-9.21<br />
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              &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1.Construction of BBa_K1523101<br />
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            </p>
+
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            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<img src="https://static.igem.org/mediawiki/2014/2/28/Z.a7.png" width="489" height="90" /></p>
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            <p>&nbsp;</p>
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            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;The size of the whole part is about 3700bp(without plasmid), we test the assembling result by PCR(sense:5'---TTCCCATCTATAATCCTCCCTGATTCTTCG---3';anti-sense:5'---GAATTCTCTAGATTACAACTGTTGTTCAAGCTGTT---3'). From the gel picture we can see the size is right.</p>
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            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<img src="https://static.igem.org/mediawiki/2014/c/cf/Z.a8.png" width="643" height="305" /></p>
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            <p>&nbsp;</p>
+
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            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2.Construction of BBa_K1523024(yieF) and BBa_K1523005(chrR)</p>
+
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<p>&nbsp;</p>
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<p>&nbsp;</p>
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<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2014.9.21-10.2<br />
+
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  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Construction of BBa_K1523021(<em>nahR</em>),  K1523022(<em>nahE</em>) and K1523023(<em>nahG</em>).</p>
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Revision as of 03:02, 17 October 2014

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