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Team:Kyoto/Notebook/DMS Synthesis
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アメフラシノート
7/31
Restriction Enzyme Digestion
| Sample | Volume | EcoR1 | Spe1 | Buffur | MilliQ | TOTAL |
|---|---|---|---|---|---|---|
| 3/24 PCR(dddD)cloning | 3.2 | 1 | 1 | 3(H) | 21.8 | 30 |
| pSB1C3 | 18.5 | 1 | 1 | 3(H) | 6.5 | 30 |
| pSB1C3(control) | 6.2 | 0 | 0 | 3(H) | 2.8 | 10 |
8/31
Gel Extraction
| Lane | DNA |
|---|---|
| 1 | 3.2 |
| 2 | 18.5 |
| 3 | 6.2 |
| 4 | 3.2 |
| 5 | 18.5 |
| 6 | 6.2 |
| 7 | 3.2 |
| 8 | 18.5 |
| 9 | 6.2 |
| 10 | 3.2 |
