Team:Kyoto/Consideration

From 2014.igem.org

Revision as of 17:21, 17 October 2014 by Mitsuaki (Talk | contribs)

count down

Consideration

Safety

About our lab form

Official "About Our Lab Form" page is HERE.

1. a) Does your country use a four-part "Safety Level" rating system for laboratories? (The system might be called in English "Risk Levels", "Bio-Safety Levels", "Containment Levels", "Bio-Security Levels", or some similar name.) (Click here for help)

If yes, which level is used for the most dangerous organisms?

Yes. Level 4 is used for the most dangerous organisms. (True for most countries in Asia, the European Union, and North/South America. This is equivalent to the WHO system.)

b) What is the Safety Level of your lab? (Use the WHO numbering system, in which Level 4 is used for the most dangerous organisms.)

Level 1 (low risk, ~= WHO BSL 1)

2. a) What type of work environments do you use to handle biological materials? Please check all that apply. (Click here for help)

  • Open benches
  • Laminar flow hood / biosafety cabinet with open front
  • "Glove box" / biosafety cabinet with closed front
  • Our team is not doing any wet-lab work (e.g. Policy & Practices Track, some teams in Software Track)
  • Other work environment (please describe):
  • We don't know the name of the work environment that we use (please comment):

b) Do you handle different materials in different work environments? If yes, please describe what materials you handle in what work environments.


3. a) What personal protective equipment do you use in your lab? Please check all that apply.

  • Lab coats
  • Gloves
  • Safety glasses / goggles
  • Full face shields
  • Surgical masks
  • Respirators (what kind?):
  • Other protective equipment (please describe):
  • We do not know how to describe our protective equipment (please comment):

b) Do you use different protective equipment for different procedures? If yes, please describe what equipment you use for what purposes.


4. How do you dispose of biological waste? (For example: liquid cell cultures, agar plates, used pipette tips.)

Safety Form

Official "Safety Form" page is HERE.

1. Your Training

a) Have your team members received any safety training yet?

Yes, we have already received safety training.

b) Please briefly describe the topics that you learned about (or will learn about) in your safety training.


c) Please give a link to the laboratory safety training requirements of your institution (college, university, community lab, etc). Or, if you cannot give a link, briefly describe the requirements.

2. Your Local Rules and Regulations

a) Who is responsible for biological safety at your institution? (You might have an Institutional Biosafety Committee, an Office of Environmental Health and Safety, a single Biosafety Officer, or some other arrangement.) Have you discussed your project with them? Describe any concerns they raised, and any changes you made in your project based on your discussion.


b) What are the biosafety guidelines of your institution? Please give a link to these guidelines, or briefly describe them if you cannot give a link.


c) In your country, what are the regulations that govern biosafety in research laboratories? Please give a link to these regulations, or briefly describe them if you cannot give a link.


3. The Organisms and Parts that You Use

Please visit this page to download a blank copy of the spreadsheet for question 3. (If you need a CSV version instead of XLS, visit this page.)

Complete the spreadsheet. Include all whole organisms that you will handle in the lab, whether you are using them as a chassis or for some other reason. Include all new or highly modified protein coding parts that you are using. If you submitted a Check-In for an organism or part, you should still include it in this spreadsheet.

You may omit non-protein-coding parts, and you may omit parts that were already in the Registry if you are using them without significant modifications.

Click here to VIEW your spreadsheet

4. Risks of Your Project Now

Please describe risks of working with the biological materials (cells, organisms, DNA, etc.) that you are using in your project. If you are taking any safety precautions (even basic ones, like rubber gloves), that is because your work has some risks, however small. Therefore, please discuss possible risks and what you have done (or might do) to minimize them, instead of simply saying that there are no risks at all.


a) Risks to the safety and health of team members, or other people working in the lab:


b) Risks to the safety and health of the general public (if any biological materials escaped from your lab):


c) Risks to the environment (from waste disposal, or from materials escaping from your lab):


d) Risks to security through malicious mis-use by individuals, groups, or countries:


e) What measures are you taking to reduce these risks? (For example: safe lab practices, choices of which organisms to use.)

5. Risks of Your Project in the Future

What would happen if all your dreams came true, and your project grew from a small lab study into a commercial/industrial/medical product that was used by many people? We invite you to speculate broadly and discuss possibilities, rather than providing definite answers. Even if the product is "safe", please discuss possible risks and how they could be addressed, rather than simply saying that there are no risks at all.


a) What new risks might arise from your project's growth? (Consider the categories of risk listed in parts a-d of the previous question: lab workers, the general public, the environment, and malicious mis-uses.) Also, what risks might arise if the knowledge you generate or the methods you develop became widely available?


b) Does your project currently include any design features to reduce risks? Or, if you did all the future work to make your project grow into a popular product, would you plan to design any new features to minimize risks? (For example: auxotrophic chassis, physical containment, etc.) Such features are not required for an iGEM project, but many teams choose to explore them.

6. Further Comments

If you are completing a Preliminary Version of your Safety Form, use this space to describe how far you have progressed in your project, and give some comments about any questions that you left blank.

You can also use this space for any other comments or additional material.

For Judges

Criteria

We meet the Gold Criteria!

Judging Form

Official "Judging Form" page is HERE.

Achievements

content

content

Acknowledgement

General Support

Tomohiko Tsuge — Institute for Chemical Research, Kyoto University
Professor Tsuge helped us all of our activity as an instructor.

Makoto Kitabatake — Institute for Virus Research, Kyoto University
Professor Kitabatake helped us all of our project and making wiki.

Shin Yonehara — Graduate School of Biostudies, Kyoto University
Professor Yonehara support our activity.

Qiu-Mei Akiyama — Department of zoology, Kyoto University
Professor Qiu-Mei Akiyama helped us to make use of laboratory in Kyoto university, and to run the laboratory, and advised our projects.

Project Advisor Support

Yoshihiko Fujita — Graduate School of Biostudies, Kyoto University
Professor Fujita helped our project and taught us how to do the experiment.

Hiromu Takematsu — Graduate School of Medicine, Kyoto University
Professor Takematsu taught us how to use HPLC and allowed us to use it.

Shigeki Sawayama — Graduate School of Agriculture, Kyoto University
Professor Sawayama gave us advices about DMS synthesis project.

Hiroshi Kubota — Department of zoology, Kyoto University
Professor Kubota taught us how to use TEM and allowed us to use it.

Funding Support

Naoki Miyano — Center for the Promotion of Interdisciplinary Education and Research
Professor Miyano advised us how to fund money from company, and suggested us new way of raising money.

Lab Support

Yoshio Koyanagi — Institute for Virus Research, Kyoto University
Professor Koyanagi provided us wet laboratory after summer vacation.

Akira Nagatani — Department of Botany, Graduate School of Science, Kyoto University
Professor Nagatani helped us to make use of laboratory in Kyoto university.

English Proofreading Support

Sayaka Dantsuji — Undergraduate School of Science, Kyoto University
Ms. Dantsuji checked our English sentenses and collected proofs.

Momoko Yamanaka — Undergraduate School of Agriculture, Kyoto University
Ms. Yamanaka checked our English sentenses and collected proofs.

Wiki Support

Suguru Kato — Undergraduate School of Science, Kyoto University
Mr. Kato helped us to make wiki.

Sponsors

Kyoto University
lent us the wet lab.
Promega KK
gave us restriction enzymes.
COSMO BIO CO., LTD.
gave us funding support.
Integrated Device Technology, Inc.
and
MEDICAL & BIOLOGICAL LABORATORIES CO., LTD.
synthesized our primers and gave us them.