Team:KAIT Japan/Results

From 2014.igem.org

(Difference between revisions)
Line 119: Line 119:
<br>
<br>
-
<H1>Creating parts of IL-10α,IL-10β<H1>
+
<H1>Creating parts of IL-10α,IL-10β</H1>
<font size="4">
<font size="4">

Revision as of 09:26, 17 October 2014

ロゴ2.png

KAIT Japan2013 Kanako.png

KAIT Japan 2014 iGEMRogo.png
New home t.jpg

Home

New Team1.jpg

Team

New 考える人.jpg

Project

New 歯車.jpg

Parts

New 111111111111111.jpg

Protocol

Ff.jpg

Notebook

New 結果.jpg

Results

New new 危険.jpg

Safety

New new ひと.jpg

Human Practice

Contents

Results


GFP+HlyA(BBa_K1414000) and HlyA+GFP(BBA_K1414001)

GFP.jpg

[Figure 1]

GFP

We used "BBa_E0040". Length of "BBa_E0040"is 920bp. Following figure 1 is result of the GFP PCR. As a result,We were able to confirm a band about 920bp by GFP PCR.



HlyA.jpg

[Figure 2]

HlyA

We used "BBa_K554002". Length of "BBa_K554002" is 186bp. Following figure 2 is result of the HlyA PCR. As a result,We were able to confirm a band about 186bp by HlyA PCR.



HlyA.jpg

[Figure 3]

GFP+HlyA.jpg

[Figure 4]

GFP+HlyA and HlyA+GFP

GFP ligated with HlyA.Length of GFP+HlyA is 920bp. Following figure 3 is GFP+HlyA. Following figure 4 is HlyA+GFP. We submitted these parts. But the function of these parts,we were not able to confirm it. Because we did not have time to experiment.


Creating parts of STAT3

STAT3's sequence includes three recognition sequences of restriction enzyme. We try to mutant this sequence,and we could succeed to mutant about one place.

Creating parts of IL-10α,IL-10β



Creating parts of HRV,IL-5Ra,IL-5Rb,AraC,Arac Promoter

140927 Arac AracP HRV colonyPCR.jpg

[Figure 1]

140929 araC araC promoter TAvector colonyPCR.jpg

[Figure 2]

Result of colony PCR

Figure 1 is colony PCR of colony transformed TA vector. Lane 1,2 are colony transformed TA vector inserted AraC gene. Lane 3,4 are colony transformed TA vector inserted AraC promoter gene. Lane 5,6,7 are colony transformed TA vector inserted HRV gene. The band of lane 5 was 325bp. Objective band size was 325bp. We are able to confirm that we get colony which has TA vecter inserted AraC promoter gene.

Figure 2 is colony PCR of colony transformed TA vector inserted AraC gene. The band of lane 1,2 were 1104bp. Objective band size was 1149bp. We are able to confirm that we get colony which has TA vector inserted AraC gene.