Team:KAIT Japan/Notebook
From 2014.igem.org
(Difference between revisions)
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:<big>'''Date:9/1'''</big> Build replica the HlyA and GFP | :<big>'''Date:9/1'''</big> Build replica the HlyA and GFP | ||
:<big>'''Date:9/1'''</big> Restriction enzyme treatment the HlyA and GFP | :<big>'''Date:9/1'''</big> Restriction enzyme treatment the HlyA and GFP | ||
- | :<big>'''Date:9/2'''</big> Restriction enzyme treatment the HlyA and GFP in 9/1 | + | :<big>'''Date:9/2'''</big> Restriction enzyme treatment the HlyA and GFP in 9/1 was NANOSEP |
:<big>'''Date:9/3'''</big> Ligation and PCR, DNA purification the HlyA and GFP | :<big>'''Date:9/3'''</big> Ligation and PCR, DNA purification the HlyA and GFP | ||
:<big>'''Date:9/4'''</big> Restriction enzyme treatment the HlyA and GFP | :<big>'''Date:9/4'''</big> Restriction enzyme treatment the HlyA and GFP | ||
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:<big>'''Date:9/17'''</big> PCR the DNA abstracted for iGEM kit.It was TA cloning and restriction enzyme treatment. | :<big>'''Date:9/17'''</big> PCR the DNA abstracted for iGEM kit.It was TA cloning and restriction enzyme treatment. | ||
:<big>'''Date:9/18'''</big> Ligation the GFP and HlyA. | :<big>'''Date:9/18'''</big> Ligation the GFP and HlyA. | ||
- | :<big>'''Date:9/19'''</big> | + | :<big>'''Date:9/19'''</big> Ligation the GFP+HlyA in 9/18 was Electrophoresis |
- | :<big>'''Date:9/20'''</big> | + | :<big>'''Date:9/19'''</big> DNA purify and PCR |
+ | :<big>'''Date:9/20'''</big> | ||
:<big>'''Date:9/21'''</big> | :<big>'''Date:9/21'''</big> | ||
:<big>'''Date:9/22'''</big> | :<big>'''Date:9/22'''</big> |
Revision as of 10:44, 15 October 2014
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Notebook
Creating parts of HlyA and GFP
Creating parts of STAT3
Creating parts of IL-10α,IL-10β
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