Team:IvyTech SouthBend IN

From 2014.igem.org

(Difference between revisions)
 
(30 intermediate revisions not shown)
Line 1: Line 1:
-
{{:Team:IvyTech_SouthBend_IN/Templates/Navigationbar}}
 
{{CSS/Main}}
{{CSS/Main}}
<html>
<html>
<style>
<style>
-
#contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .firstHeading,.visualClear {display: none;} /*-- hides default wiki settings --*/
+
#menubAar, #contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .firstHeading,.visualClear {display: none;} /*-- hides default wiki settings --*/
</style>
</style>
<!--  here ends the section that changes the default wiki template to a white full width background -->
<!--  here ends the section that changes the default wiki template to a white full width background -->
-
 
<!-- beginning of your page -->
<!-- beginning of your page -->
-
<!-- menu -->
+
<head>
-
<table id="menu" width="100%" cellspacing="0" height="135px">
+
<title>Team:IvyTech SouthBend IN</title>
-
<tr>
+
<link rel="stylesheet" type="text/css" href="https://2014.igem.org/Team:IvyTech_SouthBend_IN/css/stylesheet/standard?action=raw&ctype=text/css">
-
<td  bgColor="#FFFFFF"></td>
+
<link rel="stylesheet" type="text/css" href="https://2014.igem.org/Team:IvyTech_SouthBend_IN/css/stylesheet/standard?action=raw&amp;ctype=text/css">
-
<td valign="bottom" width="975px" align="center" bgColor="#FFFFFF">
+
</head>
-
<table align="center">
+
<div id="iheader">
-
<tr height="50px">
+
<h1><a href="https://2014.igem.org/Main_Page"><img src="http://i.imgur.com/895tLRO.png" alt="iGEM 2014" border="0"  class="logo" title="iGEM 2014"/></a> <a href="https://2014.igem.org/Team:IvyTech_SouthBend_IN"><img src="http://i.imgur.com/I2dn8CN.png" alt="Ivy Tech Community College: South Bend, IN" border="0"  class="logo" title="Ivy Tech Community College: South Bend"/></a></h1>
-
<td width="162px" align="center" onMouseOver="this.bgColor='#FEE5AD'" onMouseOut="this.bgColor='#A1DBB2'" bgColor=#A1DBB2>  
+
<h2>iGEM 2014: Ivy Tech Community College: South Bend, IN</h2>
-
<a href="https://igem.org/Team.cgi?id=1477"style="text-decoration:none;color:#1C140D">HOME </a> </td>  
+
<ul>
 +
 +
    <li><a href="https://2014.igem.org/Main_Page">iGEM <span>2014</span></a></li>
 +
   
 +
    <li><a href="https://igem.org/Team.cgi?id=1477">Ivy Tech<span>iGEM Listing</span></a></li>
 +
 +
    <li><a href="https://2014.igem.org/Team:IvyTech_SouthBend_IN" class="active">Home<span>Page</span></a></li>
 +
 +
    <li><a href="https://2014.igem.org/Team:IvyTech_SouthBend_IN/community">Local<span>Outreach</span></a></li>
 +
 +
    <li><a href="https://2014.igem.org/Team:IvyTech_SouthBend_IN/meet_our_team">Team<span>Ivy Tech</span></a></li>
 +
 +
    <li><a href="https://2014.igem.org/Team:IvyTech_SouthBend_IN/progress">Project<span>Notebook</span></a></li>
 +
 +
    <li><a href="https://igem.org/Safety/Safety_Form?team_id=1477">Project
 +
      <span>Safety</span></a></li>
 +
</ul>
 +
<p id="layoutdims">Ivy Tech Parts: <a href="http://parts.igem.org/Part:BBa_K1477014">BBa_K1477014</a> | <a href="http://parts.igem.org/Part:BBa_K1477030">BBa_K1477030</a></p>
 +
</div>
 +
<div class="ivymask fullpage">
 +
 +
  <div class="ivyleft">
 +
    <div class="ivy1">
 +
      <!-- column 1 start -->
 +
      <br />
 +
      <br />
 +
      <br />
 +
      <div id="memo"><center>Welcome, we are Ivy Tech Community College of Indiana-South Bend!</center></div>
 +
      <center><h1>Presenting the</h1></center>
 +
      <br /><div id="memo1"><center>"BETA-DATA QUANTATATA"</center></div>
 +
      <br /><center><h2>A device for the rapid detection of human waste contamination of drinking and recreational water.</h2></center>
 +
      <!-- column 1 end -->
 +
    </div>
 +
  </div>
 +
</div>
-
<td width="162px" align="center" onMouseOver="this.bgColor='#FEE5AD'" onMouseOut="this.bgColor='#A1DBB2'" bgColor=#A1DBB2>
+
<div class="ivymask threeivy">
-
<a href="http://ckmnye.wix.com/2014ivytechsouthbend" style="text-decoration:none;color:#1C140D">PROJECT</a> </td>
+
-
 
+
  <div class="ivymid">  
-
<td width="162px" align="center" onMouseOver="this.bgColor='#FEE5AD'" onMouseOut="this.bgColor='#A1DBB2'" bgColor=#A1DBB2>  
+
    <div class="ivyleft">  
-
<a href="http://parts.igem.org/Part:BBa_K1477014" style="text-decoration:none;color:#1C140D">PART_1</a></td>  
+
      <div class="ivy1">  
-
 
+
        <!-- column 1 start -->
-
<td width="162px" align="center" onMouseOver="this.bgColor='#FEE5AD'" onMouseOut="this.bgColor='#A1DBB2'" bgColor=#A1DBB2>  
+
        <p align="center"><iframe width="560" height="315" src="//www.youtube.com/embed/6AikSQlZ_nw?list=UUa1eOmuSRxiWnQCV6CElxbQ" frameborder="0" allowfullscreen></iframe></p>
-
<a href="http://parts.igem.org/Part:BBa_K1477030" style="text-decoration:none;color:#1C140D">PART_2</a></td>
+
        <br /><div id="memo3g"><center>Who will our project help?</center></div>
-
 
+
        <p>Our proposed device could not only be used to safeguard lakes, rivers, streams, swimming areas, beaches and public access sites, but potentially municipalities or anywhere clean uncontaminated water is preferred. The rapid detection and the potential low cost of the device would have a positive impact on developing countries as well as developed nations where drinking water safety is a concern.</p>
-
<td width="162px" align="center" onMouseOver="this.bgColor='#FEE5AD'" onMouseOut="this.bgColor='#A1DBB2'" bgColor=#A1DBB2>  
+
        <br /><div id="memo2g"><center>Why did we choose this project?</center></div>
-
<a href="http://ckmnye.wix.com/2014ivytechsouthbend#!about_us/cjg9" style="text-decoration:none;color:#1C140D">TEAM </a> </td>
+
        <p>The thought of creating something from the ground up as well as applying education to overcome obstacles that occur in everyday life has been a tremendous motivator. Being able to use science and engineering to make the lives of others safer is our main goal and why we chose this project.</p>
-
 
+
-
<td width="162px" align="center" onMouseOver="this.bgColor='#FEE5AD'" onMouseOut="this.bgColor='#A1DBB2'" bgColor=#A1DBB2> 
+
-
<a href="http://ckmnye.wix.com/2014ivytechsouthbend#!blog/c1y6e" style="text-decoration:none;color:#1C140D">NOTEBOOK </a> </td>
+
-
 
+
-
<td width="162px" align="center" onMouseOver="this.bgColor='#FEE5AD'" onMouseOut="this.bgColor='#A1DBB2'" bgColor=#A1DBB2> 
+
-
<a href="https://igem.org/Safety/Safety_Form?team_id=1477" style="text-decoration:none;color:#1C140D">SAFETY </a> </td>
+
-
</tr>
+
-
 
+
-
<tr> <td colspan="5"></td>
+
-
<td align="center" width="162px"></td><a href="https://igem.org/Main_Page"> </a></td>
+
-
<td align="center" width="162px">
+
-
</tr><img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px">
+
-
</table><img src="http://i.imgur.com/gDYqURm.jpg?1"55px">
+
-
 
+
-
</td>
+
-
<td  bgColor="#FFFFFF"></td>
+
-
</tr>
+
-
</table>
+
-
<!-- end of menu -->
+
-
 
+
-
 
+
-
<!-- First block of content -->
+
-
<table  id="firstblock" width="100%"  cellspacing="0" height="500px">
+
-
<tr>
+
-
<td  bgColor="#FFFFFF"></td>
+
-
<td width="975px" align="center" bgColor="#FFFFFF" >
+
-
 
+
-
<!-- You can replace this image with your team's logo! -->
+
-
<img src="http://i.imgur.com/P1ntrkN.png?1" width="500px">
+
-
<h3>Welcome!  <br> We are Team Ivy Tech Community College of Indiana-South Bend! </h3>
+
-
<p>Please click on banner links to learn more about our team and project! </p>
+
-
 
+
-
</td>
+
-
<td bgColor="#FFFFFF"></td>
+
-
</tr>
+
-
</table>
+
-
<!-- end of first block -->
+
-
 
+
-
<!--  Block of content 1 -->
+
-
<table width="100%"  cellspacing="0" height="500px">
+
-
<tr><td  bgColor="#FEE5AD"></td>
+
-
<td width="975px" align="center" bgColor="#FEE5AD" >
+
-
<table  width="975px"  cellspacing="0" height="500px">
+
-
<tr>
+
-
 
+
-
<td width="95%" bgColor="#FEE5AD"  rowspan="3" align="center">
+
-
 
+
-
<!-- Content goes here -->
+
-
<img src="https://static.igem.org/mediawiki/2014/0/0a/DwikitemplateB_dnastrand.png">
+
-
<h3>What is your project about?</h3>
+
-
<p>Our project Idea is based on the need for for a preemptive approach to fecal matter bacteriophage disease causing viruses, not the post disease acquiring reaction that is now in effect.Currently those in charge of the swimming areas wait until they are notified that someone has come down with an unknown sickness and only know they swam in the area the day before. Two to three people may have symptoms and the common denominator is swimming at the same beach or drinking the same water. A water sample is then taken, sent to a local laboratory and grown up on petri  dishes. When a beach is closed due to bacteria it is usually three days after the current full of bacteria-phages was present. Given the amount of swimming areas, beaches and public access sites, along with the number of people who frequent them, we feel that there is a need for a rapid, handheld bio-sensor that can immediately get results. </p>
+
<p></p>
<p></p>
-
</td>
+
<p></p>
-
 
+
<p></p><p></p>
-
 
+
<p><div id="memo3g"><center>Attributions:</center></div>
-
<!-- This is the back to top button -->
+
<p>The work described on these pages was performed by members of the IvyTech_SouthBend_IN team. The parts with which we constructed our biosensor were all taken from the Registry of Standard Parts to which we are grateful.  We would like to thank the Berkeley Teams for the constitutive promoter BBa-23102 and the endolysin cassette, BBa_K112806; the USTC Team for the LacZ alpha fragment; and the marvelous "Antiquity Group" for the most essential RBSs and Terminators.</p>
-
<td bgColor=#FEE5AD> </td>
+
        <!-- column 1 end -->
-
<tr><td bgColor=#FEE5AD></td></tr>
+
      </div>
-
<tr> <td  height="20px" width="5%" align="center" onMouseOver="this.bgColor='#FFFFFF'" onMouseOut="this.bgColor='#A1DBB2'" bgColor=#A1DBB2>  
+
      <div class="ivy2">  
-
<a href="#firstblock" style="text-decoration:none;color:#1C140D"> Top </a> </td></tr>
+
        <!-- column 2 start -->
-
</table>
+
        <div id="memo2"><center>What is our project about?</center></div>
-
 
+
        <p>The American Public Health Assay (APHA) for human waste contaminated water is hampered by a dependency on culturing coliform bacteria with a turn-around time exceeding one day. An assay for coliphage detection, as surrogate for coliforms, has been developed which employs the release of the host indicator cell β-galactosidase enzyme (β-gal) activity by lytic coliphage and the conversion of a colorimetric substrate. While significantly shortening the APHA time to 3 hrs, this assay requires laboratory equipment to separate phage-induced released β-gal enzymes from intact cells before addition of the substrate. To optimize this assay we have taken advantage of the α-complementation feature of β-gal. We are engineering cell lines containing either the gene fragments of LacZα or LacZΩ of β-gal on separate plasmids.</p>
-
 
+
        <p>We predict upon lysis of a mix population of cells by coliphage in the presence of substrate, the gene products will complement in trans reconstituting the β-gal activity thus eliminating a separation step in the assay. In addition we are testing the introduction of a cassette of coliphage cell lysis genes on both plasmids under the control of a coliphage promoter to accelerate the enzyme fragment release thus further reducing test time. Our goal is to design a fast-acting hand-held coliphage biosensor device that can be used by someone without any special technical expertise. We anticipate our device being useful in rapidly reporting the contamination of recreational waters in the U.S. or assuring potable water in the water sources in the developing World.</p>
-
</td>
+
        <!-- column 2 end -->
-
<td bgColor="#FEE5AD"></td>
+
      </div>
-
</tr>
+
      <div class="ivy3">  
-
</table>
+
        <!-- column 3 start -->
-
<!-- end of Block-->
+
        <div id="memo3"><center>How does our project work?</center></div>
-
 
+
        <p>The human pathogens in contaminated water are too varied to reasonably test for their presence. The presence of the normal human enteric bacteria <i>E.coli</i> has been used as a marker for contamination but their are concerns that it might not persist in the environment as long as the human pathogenic virus will. Another dependable marker of water contaminated by human waste is the presence of bacteriophage of the human intestines or so-called "coliphage."</p>
-
 
+
        <p>These have been found to be more stable under various environmental conditions. Our biosensor is designed to report the lysis of target cells from coliphage by the release of one of two fragments of the enzyme beta-galactosidase (Omega fragment). Upon release, the beta-galactosidase omega fragment joins beta-galactosidase alpha fragments that are suspended by the added contaminated water. After the two fragments join, beta-galactosidase enzyme activity is formed and an enzyme substrate present is converted to its colorimetric form. The amount of color development will be quantified by comparison with a parallel test with known amount of coliphage present.</p>
-
 
+
        <!-- column 3 end -->
-
<!--  Block of content 2 -->
+
      </div>
-
<table width="100%"  cellspacing="0" height="500px">
+
    </div>
-
<tr>
+
  </div>
-
<td  bgColor="#FACA66"></td>
+
</div>
-
<td width="975px" align="center" bgColor="#FACA66" >
+
<div id="footer">
-
 
+
<p><b>Special thanks to the Carl D. Perkins Grant, <a href="http://www.ccuri.org/">CCURI</a>, <a href="https://igem.org/Main_Page">iGEM</a>, <a href="http://ivytech.edu/">Ivy Tech</a>, the Ivy Tech Foundation, Ivy Tech Student Life, the <a href="http://www.lillyendowment.org/">Lily Endowment</a>, and <a href="https://www.steelwarehouse.com/Locations/SouthBend.aspx">Steel Warehouse</a> for financial and advisory support.</b></p>
-
<table  width="975px"  cellspacing="0" height="500px">
+
</div>
-
<tr>
+
-
<td width="95%" bgColor="#FACA66"  rowspan="3" align="center">
+
-
 
+
-
<!-- Content goes here -->
+
-
<img src="https://static.igem.org/mediawiki/2014/b/b5/Dnastrand3.png">
+
-
<h3> How does your project work?</h3>
+
-
<p> Our project uses a lytic coliphage release of the bacterial beta-galactosidase enzyme activity and the conversion of a colorimetric substrate. In the presence of lacZ alpha fragments, the LacZ omega fragments will bind to the alpha fragments creating  change in either pH or electrolyte-resistivity.</p>
+
-
 
+
-
<a href="http://ckmnye.wix.com/2014ivytechsouthbend/">Visit to Learn More</a>
+
-
 
+
-
<!-- This is the back to top section -->
+
-
<td bgColor=#FACA66> </td>
+
-
<tr><td bgColor=#FACA66></td></tr>
+
-
<tr> <td  height="20px" width="5%" align="center" onMouseOver="this.bgColor='#FFFFFF'" onMouseOut="this.bgColor='#A1DBB2'" bgColor=#A1DBB2>
+
-
<a href="#firstblock" style="text-decoration:none;color:#1C140D"> Top </a> </td></tr>
+
-
</table>
+
-
 
+
-
</td>
+
-
<td bgColor="#FACA66"></td>
+
-
</tr>
+
-
</table>
+
-
<!-- end of Block-->
+
-
 
+
-
 
+
-
 
+
-
<!--  Block of content 3 -->
+
-
<table width="100%"  cellspacing="0" height="500px">
+
-
<tr>
+
-
<td  bgColor="#F7A541"></td>
+
-
<td width="975px" align="center" bgColor="#F7A541" >
+
-
 
+
-
<table  width="975px"  cellspacing="0" height="500px">
+
-
<tr>
+
-
<td width="95%" bgColor="#F7A541"  rowspan="3" align="center">
+
-
 
+
-
<!-- Content goes here -->
+
-
 
+
-
<img src="https://static.igem.org/mediawiki/2014/7/7d/Gears.png">
+
-
<h3> Who will your project help?</h3>
+
-
<p> Our project will not only safeguard swimming areas, beaches and public access sites, but potentially safeguard municipalities or anywhere clean uncontaminated water is preferred. The rapid detection as well as the low cost prediction of the device would have a positive impact on developing countries.</p>
+
-
</td>
+
-
 
+
-
 
+
-
<!-- This is the back to top section -->
+
-
<td bgColor=#F7A541> </td>
+
-
<tr><td bgColor=#F7A541></td></tr>
+
-
<tr> <td  height="20px" width="5%" align="center" onMouseOver="this.bgColor='#FFFFFF'" onMouseOut="this.bgColor='#A1DBB2'" bgColor=#A1DBB2>
+
-
<a href="#firstblock" style="text-decoration:none;color:#1C140D"> Top </a> </td></tr>
+
-
 
+
-
</table>
+
-
 
+
-
 
+
-
</td>
+
-
<td bgColor="#F7A541"></td>
+
-
</tr>
+
-
</table>
+
-
<!-- end of Block-->
+
-
 
+
-
 
+
-
<!--  Block of content 4 -->
+
-
<table width="100%"  cellspacing="0" height="500px">
+
-
<tr>
+
-
<td  bgColor="#F45D4C"></td>
+
-
<td width="975px" align="center" bgColor="#F45D4C" >
+
-
 
+
-
<table  width="975px"  cellspacing="0" height="500px">
+
-
<tr>
+
-
<td width="95%" bgColor="#F45D4C"  rowspan="3" align="center">
+
-
<!-- Content goes here -->
+
-
 
+
-
<img src="https://static.igem.org/mediawiki/2014/c/ce/Dnastrand2.png">
+
-
<h3>Why did you choose this project?</h3>
+
-
<p> The thought of creating something from the ground up as well as applying education to overcome obstacles that occur in everyday life has been a tremendous motivator. Being able to use science and engineering to make the lives of others more safe is our main goal and why we chose this project.</p>
+
-
 
+
-
</td> 
+
-
 
+
-
 
+
-
<!-- This is the back to top section -->
+
-
<td bgColor=#F45D4C> </td>
+
-
<tr><td bgColor=#F45D4C ></td></tr>
+
-
<tr> <td  height="20px" width="5%" align="center" onMouseOver="this.bgColor='#FFFFFF'" onMouseOut="this.bgColor='#A1DBB2'" bgColor=#A1DBB2>
+
-
<a href="#firstblock" style="text-decoration:none;color:#1C140D"> Top </a> </td></tr>
+
-
 
+
-
</table>
+
-
</td>
+
-
<td bgColor="#F45D4C"></td>
+
-
</tr>
+
-
</table>
+
-
<!-- end of Block-->
+
-
 
+
-
 
+
-
 
+
-
<!-- Final block of content -->
+
-
<table  id="firstblock" width="100%"  cellspacing="0" height="200px">
+
-
<tr>
+
-
<td  bgColor="#FFFFFF"></td>
+
-
<td width="975px" align="center" bgColor="#FFFFFF" >
+
-
 
+
-
<p><img src="http://i.imgur.com/ZtG6ON9.gif"></p><p><img src="http://i.imgur.com/yE4fNG3.jpg"></p>
+
-
 
+
-
 
+
-
</td>
+
-
<td bgColor="#FFFFFF"></td>
+
-
</tr>
+
-
</table>
+
-
<!-- end of final block -->
+
</html>
</html>

Latest revision as of 01:28, 16 October 2014

Team:IvyTech SouthBend IN

iGEM 2014: Ivy Tech Community College: South Bend, IN

Ivy Tech Parts: BBa_K1477014 | BBa_K1477030




Welcome, we are Ivy Tech Community College of Indiana-South Bend!

Presenting the


"BETA-DATA QUANTATATA"

A device for the rapid detection of human waste contamination of drinking and recreational water.


Who will our project help?

Our proposed device could not only be used to safeguard lakes, rivers, streams, swimming areas, beaches and public access sites, but potentially municipalities or anywhere clean uncontaminated water is preferred. The rapid detection and the potential low cost of the device would have a positive impact on developing countries as well as developed nations where drinking water safety is a concern.


Why did we choose this project?

The thought of creating something from the ground up as well as applying education to overcome obstacles that occur in everyday life has been a tremendous motivator. Being able to use science and engineering to make the lives of others safer is our main goal and why we chose this project.

Attributions:

The work described on these pages was performed by members of the IvyTech_SouthBend_IN team. The parts with which we constructed our biosensor were all taken from the Registry of Standard Parts to which we are grateful. We would like to thank the Berkeley Teams for the constitutive promoter BBa-23102 and the endolysin cassette, BBa_K112806; the USTC Team for the LacZ alpha fragment; and the marvelous "Antiquity Group" for the most essential RBSs and Terminators.

What is our project about?

The American Public Health Assay (APHA) for human waste contaminated water is hampered by a dependency on culturing coliform bacteria with a turn-around time exceeding one day. An assay for coliphage detection, as surrogate for coliforms, has been developed which employs the release of the host indicator cell β-galactosidase enzyme (β-gal) activity by lytic coliphage and the conversion of a colorimetric substrate. While significantly shortening the APHA time to 3 hrs, this assay requires laboratory equipment to separate phage-induced released β-gal enzymes from intact cells before addition of the substrate. To optimize this assay we have taken advantage of the α-complementation feature of β-gal. We are engineering cell lines containing either the gene fragments of LacZα or LacZΩ of β-gal on separate plasmids.

We predict upon lysis of a mix population of cells by coliphage in the presence of substrate, the gene products will complement in trans reconstituting the β-gal activity thus eliminating a separation step in the assay. In addition we are testing the introduction of a cassette of coliphage cell lysis genes on both plasmids under the control of a coliphage promoter to accelerate the enzyme fragment release thus further reducing test time. Our goal is to design a fast-acting hand-held coliphage biosensor device that can be used by someone without any special technical expertise. We anticipate our device being useful in rapidly reporting the contamination of recreational waters in the U.S. or assuring potable water in the water sources in the developing World.

How does our project work?

The human pathogens in contaminated water are too varied to reasonably test for their presence. The presence of the normal human enteric bacteria E.coli has been used as a marker for contamination but their are concerns that it might not persist in the environment as long as the human pathogenic virus will. Another dependable marker of water contaminated by human waste is the presence of bacteriophage of the human intestines or so-called "coliphage."

These have been found to be more stable under various environmental conditions. Our biosensor is designed to report the lysis of target cells from coliphage by the release of one of two fragments of the enzyme beta-galactosidase (Omega fragment). Upon release, the beta-galactosidase omega fragment joins beta-galactosidase alpha fragments that are suspended by the added contaminated water. After the two fragments join, beta-galactosidase enzyme activity is formed and an enzyme substrate present is converted to its colorimetric form. The amount of color development will be quantified by comparison with a parallel test with known amount of coliphage present.