Team:Imperial/Parts

From 2014.igem.org

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                                         <p>Double cellulose binding domain (dCBD) using two cellulose binding domains from <i>Trichoderma reesei</i> cellobiohydrolases, with an N-terminal linker and internal linker sequence between the two domains which are derived from the endogenous cellobiohydrolase linker sequence. This part is in RFC(25) Freiberg fusion format to allow for easy use in protein fusions.  
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                                         <p>Double cellulose binding domain (dCBD) using two cellulose binding domains from <i>Trichoderma reesei</i> cellobiohydrolases, with an N-terminal linker and internal linker sequence between the two domains which are derived from the endogenous cellobiohydrolase linker sequence. This part is in RFC(25) Freiberg fusion format to allow for easy use in protein fusions. dCBD binding affinity for cellulose was <a href="https://2014.igem.org/Team:Imperial/Functionalisation"> characterised </a> using sfGFP fusions.
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                                         <p>A general heavy metal-binding peptide consisting of 20 Glu-Cys (EC) repeats in Freiburg format (RFC[25]) to allow for easy use in fusion proteins. We created a library of CBD fusions with this part and demonstrated [INSERT FINAL RESULTS SENTENCE HERE]
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                                         <p>A general heavy metal-binding peptide consisting of 20 Glu-Cys (EC) repeats in Freiburg format (RFC[25]) to allow for easy use in fusion proteins. We created a library of CBD fusions with this part.
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                                         <p>This part is a haemoglobin isolated from Vitreoscilla (VHb) which increases cell metabolism. We used it as one technique to try and enhance cellulose production in <i> G. xylinus </i>.
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                                         <p>This part is a haemoglobin isolated from <i>Vitreoscilla</i> (VHb) which improves the metabolic function of obligate aerobes and facultative anaerobes in low-oxygen conditions. We used this part in <i> G. xylinus </i> to try and improve growth rate and cellulose production.
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Latest revision as of 03:59, 18 October 2014

Imperial iGEM 2014

Parts

Table of Parts

<groupparts>iGEM014 Imperial</groupparts>