Team:ITESM-CEM/Safety

From 2014.igem.org

(Difference between revisions)
(Prototype team page)
Line 1: Line 1:
-
<!-- *** What falls between these lines is the Alert Box!  You can remove it from your pages once you have read and understood the alert *** -->
 
-
 
-
 
{{CSS/Main}}
{{CSS/Main}}
-
 
+
{{Team:ITESM-CEM/CSS}}
<html>
<html>
-
<!--main content -->
+
<head>
-
<table width="70%" align="center">
+
<link href='http://fonts.googleapis.com/css?family=Exo:300,400,600' rel='stylesheet' type='text/css'>
 +
<link href='http://fonts.googleapis.com/css?family=Open+Sans:400italic,700italic,400,700' rel='stylesheet' type='text/css'>
 +
<title>TEC-CEM | Safety</title>
 +
<meta http-equiv="Content-Type" content="text/html; charset=utf-8">
 +
<style type="text/css">
 +
body {
 +
background-color: #db3838;
 +
}
 +
</style>
-
<!--welcome box -->
+
</head>
-
<tr>
+
<body leftmargin="0" topmargin="0" marginwidth="0" marginheight="0">
-
<td style="border:1px solid black;" colspan="3" align="center" height="150px" bgColor=#FF404B>
+
 
-
<h1 >WELCOME TO iGEM 2014! </h1>
+
<table border="0" align="center" cellpadding="0" cellspacing="0" id="Table_01" style="background:transparent;">
-
<p>Your team has been approved and you are ready to start the iGEM season!
+
  <tr>
-
<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
+
    <td colspan="3" valign="bottom"><h3>ITESM-CEM | Medical Bioremediation</h3></td>
-
<br>
+
    <td colspan="2" rowspan="2" align="right">
-
<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:ITESM-CEM/Safety&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
+
-
</td>
+
-
</tr>
+
-
<tr> <td colspan="3" height="5px"> </td></tr>
+
<div class="header">
-
<!-- end welcome box -->
+
      <iframe src="https://2014.igem.org/Team:ITESM-CEM/HEADER" width="235" height="77" frameborder="0" scrolling="no" seamless></iframe>
-
<tr>  
+
      </div>
-
<!--navigation menu -->
 
-
<td align="center" colspan="3">
 
-
 
-
<table  width="100%">
 
-
<tr heigth="15px"></tr>
 
-
<tr heigth="75px">
 
-
 
-
 
-
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7> 
 
-
<a href="https://2014.igem.org/Team:ITESM-CEM"style="color:#000000">Home </a> </td>
 
-
 
-
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
 
-
<a href="https://2014.igem.org/Team:ITESM-CEM/Team"style="color:#000000"> Team </a> </td>
 
-
 
-
<td style="border:1px solid black;" align="center"  height ="45px"  onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
 
-
<a href="https://igem.org/Team.cgi?year=2014&team_name=ITESM-CEM"style="color:#000000"> Official Team Profile </a></td>
 
-
 
-
<td style="border:1px solid black" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7> 
 
-
<a href="https://2014.igem.org/Team:ITESM-CEM/Project"style="color:#000000"> Project</a></td>
 
-
 
-
<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
 
-
<a href="https://2014.igem.org/Team:ITESM-CEM/Parts"style="color:#000000"> Parts</a></td>
 
-
 
-
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
 
-
<a href="https://2014.igem.org/Team:ITESM-CEM/Modeling"style="color:#000000"> Modeling</a></td>
 
-
 
-
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7> 
 
-
<a href="https://2014.igem.org/Team:ITESM-CEM/Notebook"style="color:#000000"> Notebook</a></td>
 
-
 
-
<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
 
-
<a href="https://2014.igem.org/Team:ITESM-CEM/Safety"style=" color:#000000"> Safety </a></td>
 
-
 
-
<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
 
-
<a href="https://2014.igem.org/Team:ITESM-CEM/Attributions"style="color:#000000"> Attributions </a></td>
 
-
 
-
 
-
<td align ="center"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"></a> </td>
 
-
</tr>
 
-
</table>
 
-
<!--end navigation menu -->
 
-
</tr>
 
-
</tr>
 
</td>
</td>
 +
    <td><img src="images/spacer.gif" width="1" height="43" alt=""></td>
 +
  </tr>
 +
  <tr>
 +
    <td colspan="3" rowspan="2"><h1>Safety
 +
        <h1n> 4102</h1n></h1></td>
 +
    <td><img src="images/spacer.gif" width="1" height="34" alt=""></td>
 +
  </tr>
 +
  <tr>
 +
    <td rowspan="4">&nbsp;</td>
 +
    <td rowspan="4" align="right" valign="top">
-
<tr> <td colspan="3" height="15px"> </td></tr>
+
<div class="headerSocial">
-
<tr><td bgColor="#e7e7e7" colspan="3" height="1px"> </tr>
+
    <iframe src="https://2014.igem.org/Team:ITESM-CEM/SOCIAL" width="165" height="34" frameborder="0" scrolling="no" seamless></iframe>    
-
<tr> <td colspan="3" height="5px"> </td></tr>
+
      </div>
 +
   
 +
    <td><img src="images/spacer.gif" width="1" height="6" alt=""></td>
 +
  </tr>
 +
  <tr>
 +
    <td colspan="3" rowspan="3" align="left" valign="top"><ul>
 +
      <sub><a href="https://2014.igem.org/Team:ITESM-CEM/Interlab">Interlab</a></sub>
 +
      <sub><a href="https://2014.igem.org/Team:ITESM-CEM/Interlab">Existing GFP device</a></sub>
 +
      <sub><a href="https://2014.igem.org/Team:ITESM-CEM/Interlab">New GFP device 1</a></sub>
 +
      <sub><a href="https://2014.igem.org/Team:ITESM-CEM/Interlab">New GFP device 2</a></sub>
 +
    </ul></td>
 +
    <td><img src="images/spacer.gif" width="1" height="1" alt=""></td>
 +
  </tr>
 +
  <tr>
 +
    <td><img src="images/spacer.gif" width="1" height="3" alt=""></td>
 +
  </tr>
 +
  <tr>
 +
    <td><img src="images/spacer.gif" width="1" height="28" alt=""></td>
 +
  </tr>
 +
  <tr>
 +
    <td colspan="5" valign="top">
-
<!--safety content-->
 
-
<tr><td > <h3> Welcome! </h3></td>
 
-
<td ></td >
 
-
<td > <h3> Timeline</h3></td>
 
-
</tr>
 
-
 
-
<tr>
 
-
<td width="45%"  valign="top">
 
-
<p> Visit the <a href="https://2014.igem.org/Safety" >Safety Hub</a> to see this year's safety requirements. The Safety Hub is the central page for everything related to safety in iGEM. You can also go there to learn about general biosafety topics, and how to think about the future implications of your project.</p>
 
 +
<!--INICIO CONTENIDO -->
 +
<div style="background-color: #f3f3e2;">
 +
<table width="100%" border="0" id="ContenidoSecciones">
<br>
<br>
-
<h3> Edit this page!</h3>
+
<h2>Overview</h2>
-
<p>
+
      <p>During 2014 iGEM competition, teams were requested to analyse the efficiency of 3 different genetic devices (BioBricks) using GFP as a marker of gene expression. Here, iGEM ITESM CEM team presents the results of this interlab fluorescence measurement study.
-
Please use this page to write about anything related to safety in your project. <!--Be sure to talk about both
+
<br><br>
-
<ul>
+
-
<li> <a href=" ">Learn about lab Safety for Today</a></li>
+
-
<li> <a href="">Learn about Safety for the future of your project.</a></li>
+
-
</ul>
+
-
-->
+
-
</p>
+
-
<h3> Your Lab </h3>
+
The three devices analysed are composed by a variable promoter, a gene encoding a mutant Green Fluorescence Protein (GFP) used as a marker of expression, and a plasmid backbone. Two promoters (BBa_J23101 and BBa_J23115, recently renamed BBa_K823005 and BBa_K823012 at iGEM’s catalogue) are used, both of them being members of a family of constitutive promoters described by Chris Anderson, member of iGEM Berkley Team, in 2006 (1). This family of parts is registered at the catalogue under the alphanumeric codes BBa_J23100 – BBa_J23119.
 +
<br><br>
 +
Two different plasmid backbones are used: a low-copy (psB3K3) and a high-copy plasmid (psB1C3). The GFP-expressing BioBrick remains the same for all devices (registered at the catalog as BBa_E0240), and is composed of a ribosome binding site (RBS), a mutant GFP gene, and two termination sequences.
 +
<br><br>
 +
The aim of this study is to report the relative efficiency of the following genetic devices:<br><br>
 +
1) Promoter BBa_K823005 in low-copy plasmid psB3K3<br>
 +
2) Promoter BBa_K823005 in high-copy plasmig psB1C3<br>
 +
3) Promoter BBa_K823012 in high-copy plasmid psB1C3<br><br>
 +
In order to do so, GFP (BBa_E0240) is used as a marker of gene expression or reporter gene, because of the ease of fluorescence measurement experiments.<br><br>
 +
GFP has long been used as a reporter of patterns of gene expression in both prokaryotes, were it is useful for characterization of promoters, enhancers and terminators; and in eukaryotes, were tissue-specific or time-specific gene expression can be traced (2). The basis of this procedure is the usage of GFP’s fluorescence as a reporter of activity of promoters and enhancers; the relative fluorescence of cells at different experimental conditions can be compared with statistical techniques, and so the efficiency of the parts can be tested.
 +
<br><br>
 +
<br><br><br><br><br><br>
 +
</p>
 +
</table>
 +
      <p>&nbsp;</p></td>
 +
    <td><img src="images/spacer.gif" width="1" height="509" alt=""></td>
 +
  </tr>
 +
  <tr>
 +
    <td><img src="images/spacer.gif" width="240" height="1" alt=""></td>
 +
    <td><img src="images/spacer.gif" width="544" height="1" alt=""></td>
 +
    <td><img src="images/spacer.gif" width="133" height="1" alt=""></td>
 +
    <td><img src="images/spacer.gif" width="70" height="1" alt=""></td>
 +
    <td><img src="images/spacer.gif" width="165" height="1" alt=""></td>
 +
    <td></td>
 +
  </tr>
 +
</table>
-
<p> Use this section to tell us about your laboratory. Where is it located? What sort of equipment do you use every day? Have you decorated it for the summer? How do you look wearing a lab coat? Take pictures! Show off your space! </p>  
+
</div>
-
<!--
+
<!--fin main content -->
-
<gallery>
+
-
Image:Example2_Lab_1.png|The building our lab is in!
+
-
Image:Example2_Lab_2.png|The inside of our lab!
+
-
Image:Example2_Lab_3.png|Team Member 3 doing an experiment
+
-
Image:Example2_Lab_4.png|Working in biosafety cabinets
+
-
Image:Example2_Lab_5.png|Team all gloved up and ready for work!
+
-
Image:Example2_Lab_6.png|Equipment that we use to do SCIENCE!
+
-
Image:Example2_Lab_7.png|We decorated this part of our lab
+
-
Image:Example2_Lab_8.png|Whatever else you want
+
-
</gallery>-->
+
-
</td>
 
-
<td></td>
 
-
<td width="45%" valign="top">  
+
  <table width="100%" border="0" align="center" cellpadding="0" cellspacing="0" id="Table_" dwcopytype="CopyTableRow">
 +
    <tr>
 +
      <td colspan="5" align="left" valign="bottom">
 +
      <div class="footer">
 +
      <iframe src="https://2014.igem.org/Team:ITESM-CEM/FOOTER" width="500" height="82" frameborder="0" scrolling="no" seamless></iframe></div>
 +
      </td>
 +
      <td width="37%" align="right" valign="bottom">
 +
     
 +
      <div class="PLASMENU">
 +
      <iframe src="https://2014.igem.org/Team:ITESM-CEM/POPMENU" width="614" height="614" frameborder="0" scrolling="no" seamless></iframe>
 +
      </div>
 +
     
 +
      </td>
 +
    </tr>
 +
  </table>
 +
</body>
-
<ul>
 
-
<li> <b>Now :</b>  Read the <a href="https://2014.igem.org/Safety">Safety Hub </a> and learn about safety in iGEM. Ask questions by emailing safety at <i> igem DOT org </i>. </li>
 
-
<li><b>Now - Jamboree:</b> Complete <b>Check-Ins</b> and receive approval before acquiring and using certain materials in your lab</li>
 
-
<li><b>Now - Wiki Freeze:</b> Edit this Safety page to tell us about what you're doing</li>
 
-
<li><b>June 9: </b>Submit the About Our Lab form.</li>
 
-
<li><b>Let us know by June 25 </b>if you will need an extension on the Preliminary Version, or your Preliminary Version will be significantly incomplete.</li>
 
-
<li><b>June 30: </b>Submit the Preliminary Version of the <b>Safety Form</b>.</li>
 
-
<li>Participate in Virtual Open Office Hours to ask questions and discuss safety topics (exact date to be determined).</li>
 
-
<li><b>September 1:</b> Submit the Final Version of the Safety Form.</li>
 
-
<li><b>October: </b> Wiki freeze (exact date to be determined)</li>
 
-
<li><b>October 30 - November 3: </b>GIANT JAMBOREE!</li>
 
-
</ul>
 
-
</td>
 
-
 
-
</tr>
 
-
 
-
 
-
 
-
 
-
<tr>
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
</table>
 
</html>
</html>

Revision as of 18:07, 24 September 2014

TEC-CEM | Safety

ITESM-CEM | Medical Bioremediation

Safety 4102

 

Overview

During 2014 iGEM competition, teams were requested to analyse the efficiency of 3 different genetic devices (BioBricks) using GFP as a marker of gene expression. Here, iGEM ITESM CEM team presents the results of this interlab fluorescence measurement study.

The three devices analysed are composed by a variable promoter, a gene encoding a mutant Green Fluorescence Protein (GFP) used as a marker of expression, and a plasmid backbone. Two promoters (BBa_J23101 and BBa_J23115, recently renamed BBa_K823005 and BBa_K823012 at iGEM’s catalogue) are used, both of them being members of a family of constitutive promoters described by Chris Anderson, member of iGEM Berkley Team, in 2006 (1). This family of parts is registered at the catalogue under the alphanumeric codes BBa_J23100 – BBa_J23119.

Two different plasmid backbones are used: a low-copy (psB3K3) and a high-copy plasmid (psB1C3). The GFP-expressing BioBrick remains the same for all devices (registered at the catalog as BBa_E0240), and is composed of a ribosome binding site (RBS), a mutant GFP gene, and two termination sequences.

The aim of this study is to report the relative efficiency of the following genetic devices:

1) Promoter BBa_K823005 in low-copy plasmid psB3K3
2) Promoter BBa_K823005 in high-copy plasmig psB1C3
3) Promoter BBa_K823012 in high-copy plasmid psB1C3

In order to do so, GFP (BBa_E0240) is used as a marker of gene expression or reporter gene, because of the ease of fluorescence measurement experiments.

GFP has long been used as a reporter of patterns of gene expression in both prokaryotes, were it is useful for characterization of promoters, enhancers and terminators; and in eukaryotes, were tissue-specific or time-specific gene expression can be traced (2). The basis of this procedure is the usage of GFP’s fluorescence as a reporter of activity of promoters and enhancers; the relative fluorescence of cells at different experimental conditions can be compared with statistical techniques, and so the efficiency of the parts can be tested.