Team:ITESM-CEM/Parts

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<td style="border:1px solid black;" colspan="3" align="center" height="150px" bgColor=#FF404B>
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    <td colspan="3" valign="bottom"><h3>ITESM-CEM | Enzy7-K me</h3></td>
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<h1 >WELCOME TO iGEM 2014! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season!
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:ITESM-CEM/Parts&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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    <td colspan="3" rowspan="2"><h1>Parts
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        <h1n> 3256</h1n></h1></td>
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      <sub><a href="https://2014.igem.org/Team:ITESM-CEM/Parts" style="color: #FFF;">Our Parts</a></sub>
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      <sub><a href="https://2014.igem.org/Team:ITESM-CEM/List">List of our parts</a></sub>
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<a href="https://2014.igem.org/Team:ITESM-CEM/Team"style="color:#000000"> Team </a> </td>
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<a href="https://igem.org/Team.cgi?year=2014&team_name=ITESM-CEM"style="color:#000000"> Official Team Profile </a></td>
 
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<h2>Our Parts</h2>
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<a href="https://2014.igem.org/Team:ITESM-CEM/Project"style="color:#000000"> Project</a></td>
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<p style="text-align: justify; text-justify: inter-word;"> The main goal of our project was to establish the construct which will help us to metabolize 7-ketocholesterol, consisting in the use of three specific enzymes, but for further applications we submitted them in single modules. This will serve as the basis of a future library for standardized work related to atherosclerosis.  
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<a href="https://2014.igem.org/Team:ITESM-CEM/Modeling"style="color:#000000"> Modeling</a></td>
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<h2>Cholesterol Oxidase</h2>
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<a href="https://2014.igem.org/Team:ITESM-CEM/Notebook"style="color:#000000"> Notebook</a></td>
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<p style="text-align: justify; text-justify: inter-word;"> This enzyme was first detected in <u>Chromobacterium sp.</u> We introduced it in a plasmid backbone with chloramphenicol resistance: pSB1C3. Its length is of 1871 nucleotides and its codons were optimized in order to use it on <u>E. coli</u>, it already included a stop codon for transcription, it was also modified by the addition of a glycosilation site (NIT) and the peptide signal of human S-cathepsin.   
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<h2>Oxoacyl Reductase</h2>
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<a href="https://2014.igem.org/Team:ITESM-CEM/Safety"style=" color:#000000"> Safety </a></td>
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<p style="text-align: justify; text-justify: inter-word;"> This enzyme was detected in <u>Rhodococcus jostii </u>. We introduced it in a plasmid backbone with chloramphenicol resistance: pSB1C3. Its length is of 1007 nucleotides and its codons were optimized in order to use it on <u>E. coli</u>, it already included a stop codon for transcription, it was also modified by the addition of a glycosilation site (NIT) and the peptide signal of human S-cathepsin.
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<h2>7-dehydratase</h2>
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<a href="https://2014.igem.org/Team:ITESM-CEM/Attributions"style="color:#000000"> Attributions </a></td>
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<p style="text-align: justify; text-justify: inter-word;"> This enzyme (7-alpha dehydratase) was detected in <u>Rhodococcus jostii</u> . We introduced it in a plasmid backbone with chloramphenicol resistance: pSB1C3. Its length is of 602 nucleotides and its codons were optimized in order to use it on <u>E.coli</u>, it already included a stop codon for trancription, it was also modified by the addition of a glycosilation site (NIT) and the peptide signal of human S-cathepsin.
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<h2>Neomycin Resistance</h2>
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<p style="text-align: justify; text-justify: inter-word;"> This selective marker was obtained from an mammalian expression vector. NeoR's length is 855 nucleotides and it was isolated from pcDNA3.1(-)/myc-His A.
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<h2>BGHPA</h2>
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<p style="text-align: justify; text-justify: inter-word;"> Bovine Growth Hormone Polyadenilation Signal for nuclease resistance. Translation terminator for eukaryotic cells.
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<p style="text-align: justify; text-justify: inter-word;"> Constitutive promoter from Cytomegalovirus, this promoter works on eukaryotic cells, driving protein expression.
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An important aspect of the iGEM competition is the use and creation of standard  biological parts. Each team will make new parts during iGEM and will submit them to the <a href="http://partsregistry.org"> Registry of Standard Biological Parts</a>. The iGEM software provides an easy way to present the parts your team has created. The "groupparts" tag will generate a table with all of the parts that your team adds to your team sandbox. 
 
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<strong>Note that if you want to document a part you need to document it on the <a href="http://partsregistry.org Registry"> Registry</a>, not on your team wiki.</strong> Future teams and other users and are much more likely to find parts on the Registry than on your team wiki.
 
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Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without a need to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.
 
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<h3>When should you put parts into the Registry?</h3>
 
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As soon as possible! We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better recall you will have of all details surrounding your parts. Remember you don't need to send us the DNA to create an entry for a part on the Registry. However, you must send us the sample/DNA before the Jamboree. Only parts for which you have sent us samples/DNA are eligible for awards and medal requirements.
 
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The information needed to initially create a part on the Registry is:
 
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<li>Part Name</li>
 
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. Check out part <a href="http://parts.igem.org/Part:BBa_K404003">BBa_K404003</a> for an excellent example of a highly characterized part.
 
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You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry"> Add a Part to the Registry</a> link.
 
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<tr><td colspan="3" > <h3> Parts Table</h3></td></tr>
 
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Any parts your team has created will appear in this table below:</td></tr>
 
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<groupparts>iGEM013 ITESM-CEM</groupparts>
 

Latest revision as of 02:12, 18 October 2014

TEC-CEM | Parts

ITESM-CEM | Enzy7-K me

Parts 3256
 

Our Parts

The main goal of our project was to establish the construct which will help us to metabolize 7-ketocholesterol, consisting in the use of three specific enzymes, but for further applications we submitted them in single modules. This will serve as the basis of a future library for standardized work related to atherosclerosis.


Cholesterol Oxidase

This enzyme was first detected in Chromobacterium sp. We introduced it in a plasmid backbone with chloramphenicol resistance: pSB1C3. Its length is of 1871 nucleotides and its codons were optimized in order to use it on E. coli, it already included a stop codon for transcription, it was also modified by the addition of a glycosilation site (NIT) and the peptide signal of human S-cathepsin.


Oxoacyl Reductase

This enzyme was detected in Rhodococcus jostii . We introduced it in a plasmid backbone with chloramphenicol resistance: pSB1C3. Its length is of 1007 nucleotides and its codons were optimized in order to use it on E. coli, it already included a stop codon for transcription, it was also modified by the addition of a glycosilation site (NIT) and the peptide signal of human S-cathepsin.


7-dehydratase

This enzyme (7-alpha dehydratase) was detected in Rhodococcus jostii . We introduced it in a plasmid backbone with chloramphenicol resistance: pSB1C3. Its length is of 602 nucleotides and its codons were optimized in order to use it on E.coli, it already included a stop codon for trancription, it was also modified by the addition of a glycosilation site (NIT) and the peptide signal of human S-cathepsin.


Neomycin Resistance

This selective marker was obtained from an mammalian expression vector. NeoR's length is 855 nucleotides and it was isolated from pcDNA3.1(-)/myc-His A.


BGHPA

Bovine Growth Hormone Polyadenilation Signal for nuclease resistance. Translation terminator for eukaryotic cells.


PCMV

Constitutive promoter from Cytomegalovirus, this promoter works on eukaryotic cells, driving protein expression.





 

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