Team:ITESM-CEM/Parts

From 2014.igem.org

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       <sub>Submenú 1</sub><sub>Submenú 2</sub><sub>Submenú 3</sub>
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       <sub><a href="https://2014.igem.org/Team:ITESM-CEM/Parts" style="color: #FFF;">Our Parts</a></sub>
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    </ul></td>
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      <sub><a href="https://2014.igem.org/Team:ITESM-CEM/List">List of our parts</a></sub>
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        </ul></td>
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<table width="100%" border="0" id="ContenidoSecciones">
<table width="100%" border="0" id="ContenidoSecciones">
<div style="background-color: #f3f3e2; style="width:95%">
<div style="background-color: #f3f3e2; style="width:95%">
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<!--INICIO CONTENIDO-->
<!--INICIO CONTENIDO-->
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<br>
 
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<table class="table table-striped">
 
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<tr>
 
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<th>Name</th>
 
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<th>Type</th>
 
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<th>Description</th>
 
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<th>Designer</th>
 
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<th>Length (bp)</th>
 
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</tr>
 
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<!-- Favorite-Name-Type-Description-Designer-Length-->
 
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<tr>
 
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<td><a href="http://parts.igem.org/Part:BBa_K1313000">BBa_K1313000</a></td>
 
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<td>Enzyme</td>
 
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<td>Colox</td>
 
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<td>Eddie Cano Gámez</td>
 
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<td>1</td>
 
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</tr>
 
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<tr>
 
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<td> <a href="http://parts.igem.org/Part:BBa_K1313001">BBa_K1313001</a></td>
 
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<td>Enzyme</td>
 
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<td>OxRed</td>
 
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<td>Eddie Cano Gámez</td>
 
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<td>1</td>
 
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</tr>
 
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<tr>
 
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<td> <a href="http://parts.igem.org/Part:BBa_K1313002"> BBa_K1313002 </a> </td>
 
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<td>Enzyme</td>
 
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<td>Dehydratase</td>
 
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<td>Eddie Cano Gámez</td>
 
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<td>1</td>
 
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</tr>
 
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<tr>
 
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<td> <a href="http://parts.igem.org/Part:BBa_K1313003"> BBa_K1313003 </a> </td>
 
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<td>Antibiotic Resistance</td>
 
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<td>NeoR</td>
 
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<td>Carlos Meza Ramírez</td>
 
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<td>1</td>
 
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</tr>
 
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<tr>
 
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<td> <a href="http://parts.igem.org/Part:BBa_K1313004"> BBa_K1313004 </a> </td>
 
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<td>Promoter</td>
 
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<td>pCMV/td>
 
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<td>Oliva Sánchez Montesinos</td>
 
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<td>1</td>
 
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</tr>
 
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<tr>
 
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<td> <a href="http://parts.igem.org/Part:BBa_K1313004"> BBa_K1313005 </a> </td>
 
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<td>Stop</td>
 
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<td>BGH PA/td>
 
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<td>Oliva Sánchez Montesinos</td>
 
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<td>1</td>
 
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</tr>
 
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</table>
 
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<h2> ColOx </h2>
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<h2>Our Parts</h2>
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<P> Cholesterol oxidase is a well characterized enzyme.    
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<iframe align="right" width="660" height="515" src="//www.youtube.com/embed/1mFwPDbb7UY" frameborder="0" allowfullscreen></iframe>
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</p>
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<p style="text-align: justify; text-justify: inter-word;"> The main goal of our project was to establish the construct which will help us to metabolize 7-ketocholesterol, consisting in the use of three specific enzymes, but for further applications we submitted them in single modules. This will serve as the basis of a future library for standardized work related to atherosclerosis.   
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</p><br>
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<h2>Cholesterol Oxidase</h2>
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<p style="text-align: justify; text-justify: inter-word;"> This enzyme was first detected in <u>Chromobacterium sp.</u> We introduced it in a plasmid backbone with chloramphenicol resistance: pSB1C3. Its length is of 1871 nucleotides and its codons were optimized in order to use it on <u>E. coli</u>, it already included a stop codon for transcription, it was also modified by the addition of a glycosilation site (NIT) and the peptide signal of human S-cathepsin.   
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</p> <br>
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<h2>Oxoacyl Reductase</h2>
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<p style="text-align: justify; text-justify: inter-word;"> This enzyme was detected in <u>Rhodococcus jostii </u>. We introduced it in a plasmid backbone with chloramphenicol resistance: pSB1C3. Its length is of 1007 nucleotides and its codons were optimized in order to use it on <u>E. coli</u>, it already included a stop codon for transcription, it was also modified by the addition of a glycosilation site (NIT) and the peptide signal of human S-cathepsin.
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</p> <br>
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<h2>7-dehydratase</h2>
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<p style="text-align: justify; text-justify: inter-word;"> This enzyme (7-alpha dehydratase) was detected in <u>Rhodococcus jostii</u> . We introduced it in a plasmid backbone with chloramphenicol resistance: pSB1C3. Its length is of 602 nucleotides and its codons were optimized in order to use it on <u>E.coli</u>, it already included a stop codon for trancription, it was also modified by the addition of a glycosilation site (NIT) and the peptide signal of human S-cathepsin.
 +
</p> <br>
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<h2>Neomycin Resistance</h2>
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<p style="text-align: justify; text-justify: inter-word;"> This selective marker was obtained from an mammalian expression vector. NeoR's length is 855 nucleotides and it was isolated from pcDNA3.1(-)/myc-His A.
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</p><br>
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<h2>BGHPA</h2>
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<p style="text-align: justify; text-justify: inter-word;"> Bovine Growth Hormone Polyadenilation Signal for nuclease resistance. Translation terminator for eukaryotic cells.
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</p><br>
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<h2>PCMV</h2>
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<p style="text-align: justify; text-justify: inter-word;"> Constitutive promoter from Cytomegalovirus, this promoter works on eukaryotic cells, driving protein expression.
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</p><br><br><br>
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<br>
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Latest revision as of 02:12, 18 October 2014

TEC-CEM | Parts

ITESM-CEM | Enzy7-K me

Parts 3256

 

Our Parts

The main goal of our project was to establish the construct which will help us to metabolize 7-ketocholesterol, consisting in the use of three specific enzymes, but for further applications we submitted them in single modules. This will serve as the basis of a future library for standardized work related to atherosclerosis.


Cholesterol Oxidase

This enzyme was first detected in Chromobacterium sp. We introduced it in a plasmid backbone with chloramphenicol resistance: pSB1C3. Its length is of 1871 nucleotides and its codons were optimized in order to use it on E. coli, it already included a stop codon for transcription, it was also modified by the addition of a glycosilation site (NIT) and the peptide signal of human S-cathepsin.


Oxoacyl Reductase

This enzyme was detected in Rhodococcus jostii . We introduced it in a plasmid backbone with chloramphenicol resistance: pSB1C3. Its length is of 1007 nucleotides and its codons were optimized in order to use it on E. coli, it already included a stop codon for transcription, it was also modified by the addition of a glycosilation site (NIT) and the peptide signal of human S-cathepsin.


7-dehydratase

This enzyme (7-alpha dehydratase) was detected in Rhodococcus jostii . We introduced it in a plasmid backbone with chloramphenicol resistance: pSB1C3. Its length is of 602 nucleotides and its codons were optimized in order to use it on E.coli, it already included a stop codon for trancription, it was also modified by the addition of a glycosilation site (NIT) and the peptide signal of human S-cathepsin.


Neomycin Resistance

This selective marker was obtained from an mammalian expression vector. NeoR's length is 855 nucleotides and it was isolated from pcDNA3.1(-)/myc-His A.


BGHPA

Bovine Growth Hormone Polyadenilation Signal for nuclease resistance. Translation terminator for eukaryotic cells.


PCMV

Constitutive promoter from Cytomegalovirus, this promoter works on eukaryotic cells, driving protein expression.