Team:ITESM-CEM/Parts

From 2014.igem.org

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       <sub>Submenú 1</sub><sub>Submenú 2</sub><sub>Submenú 3</sub>
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       <sub><a href="https://2014.igem.org/Team:ITESM-CEM/Parts" style="color: #FFF;">Our Parts</a></sub>
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    </ul></td>
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      <sub><a href="https://2014.igem.org/Team:ITESM-CEM/List" style="color: #FFF;">List of our parts</a></sub>
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        </ul></td>
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<div style="background-color: #f3f3e2; style="width:95%">
<!--INICIO CONTENIDO-->
<!--INICIO CONTENIDO-->
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<h2>Our Parts</h2>
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<p style="text-align: justify; text-justify: inter-word> The main goal of our project was to establish the construct which will help us to degrade 7-ketocholesterol consisting in the use of three specific enzymes, but for further applications we submitted them in single modules. This will serve as the basis of a future library for standardized work related with atherosclerosis.   
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</p><br><br>
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<br>
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<h2>Cholesterol Oxidase</h2>
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<table class="table table-striped">
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<p style="text-align: justify; text-justify: inter-word> This enzyme was first detected in Chromobacterium sp. We introduced it in a plasmid backbone with chloramphenicol resistance. Its length is of 1871 nucleotides and its codons were optimized in order to use it on E.coli, it already included a stop codon, it was also modified by the addition of a glycosilation site and the peptide signal of human cathepsin.  
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</p> <br><br>
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<th>Name</th>
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<th>Type</th>
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<th>Description</th>
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<th>Designer</th>
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<th>Length (bp)</th>
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<td><a href="http://parts.igem.org/Part:BBa_K1313000">BBa_K1313000</a></td>
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<td>Enzyme</td>
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<td>Colox</td>
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<td>Eddie Cano Gámez</td>
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<td>1</td>
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</tr>
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<td> <a href="http://parts.igem.org/Part:BBa_K1313001">BBa_K1313001</a></td>
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<td>Enzyme</td>
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<td>OxRed</td>
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<td>Eddie Cano Gámez</td>
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<td>1</td>
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</tr>
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<tr>
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<td> <a href="http://parts.igem.org/Part:BBa_K1313002"> BBa_K1313002 </a> </td>
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<td>Enzyme</td>
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<td>Dehydratase</td>
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<td>Eddie Cano Gámez</td>
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<td>1</td>
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</tr>
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<tr>
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<td> <a href="http://parts.igem.org/Part:BBa_K1313003"> BBa_K1313003 </a> </td>
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<td>Antibiotic Resistance</td>
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<td>NeoR</td>
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<td>Carlos Meza Ramírez</td>
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<td>1</td>
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</tr>
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<tr>
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<td> <a href="http://parts.igem.org/Part:BBa_K1313004"> BBa_K1313004 </a> </td>
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<td>Promoter</td>
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<td>pCMV</td>
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<td>Oliva Sánchez Montesinos</td>
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<td>1</td>
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<tr>
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<td> <a href="http://parts.igem.org/Part:BBa_K1313004"> BBa_K1313005 </a> </td>
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<td>Stop</td>
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<td>BGH PA</td>
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<td>Oliva Sánchez Montesinos</td>
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<td>1</td>
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</tr>
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</table>
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<h2> ColOx </h2>
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<h2>Oxoacyl Reductase</h2>
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<p style="text-align: justify; text-justify: inter-word> This enzyme was detected in Rhodococcus jostii . We introduced it in a plasmid backbone with chloramphenicol resistance. Its length is of 1007 nucleotides and its codons were optimized in order to use it on E.coli, it already included a stop codon, it was also modified by the addition of a glycosilation site and the peptide signal of human cathepsin.
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</p> <br><br>
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<P> Cholesterol oxidase is a well characterized enzyme.    
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<h2>7-dehydratase</h2>
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</p>
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<p style="text-align: justify; text-justify: inter-word> This enzyme (7-alpha dehydratase) was detected in Rhodococcus jostii . We introduced it in a plasmid backbone with chloramphenicol resistance. Its length is of 602 nucleotides and its codons were optimized in order to use it on E.coli, it already included a stop codon, it was also modified by the addition of a glycosilation site and the peptide signal of human cathepsin.
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</p> <br><br>
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<h2>Neomycin Resistance</h2>
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<p style="text-align: justify; text-justify: inter-word> This selective marker was gotten from a plasmid for mammalian expression, its length is of 855 nucleotides,and it was isolated from pcDNA3.1(+)/myc-His A.
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</p><br><br>
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<h2>BGHPA</h2>
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<p style="text-align: justify; text-justify: inter-word> Stop coding for eucaryotic cells
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</p><br><br>
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<h2>PCMV</h2>
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<p style="text-align: justify; text-justify: inter-word> Promoter from Human Cytomegalovirus, this promoter works only on eucaryotic cells.
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</p><br><br><br>
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<br>
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Revision as of 15:19, 2 October 2014

TEC-CEM | Parts

ITESM-CEM | Enzy7-K me

Parts 3256
 

Our Parts

This enzyme was first detected in Chromobacterium sp. We introduced it in a plasmid backbone with chloramphenicol resistance. Its length is of 1871 nucleotides and its codons were optimized in order to use it on E.coli, it already included a stop codon, it was also modified by the addition of a glycosilation site and the peptide signal of human cathepsin.



Oxoacyl Reductase

This enzyme (7-alpha dehydratase) was detected in Rhodococcus jostii . We introduced it in a plasmid backbone with chloramphenicol resistance. Its length is of 602 nucleotides and its codons were optimized in order to use it on E.coli, it already included a stop codon, it was also modified by the addition of a glycosilation site and the peptide signal of human cathepsin.



Neomycin Resistance

Stop coding for eucaryotic cells



PCMV

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