Team:ITESM-CEM/Modeling

From 2014.igem.org

(Difference between revisions)
 
(36 intermediate revisions not shown)
Line 6: Line 6:
<head>
<head>
<link href='http://fonts.googleapis.com/css?family=Exo:300,400,600' rel='stylesheet' type='text/css'>
<link href='http://fonts.googleapis.com/css?family=Exo:300,400,600' rel='stylesheet' type='text/css'>
 +
<link href='http://fonts.googleapis.com/css?family=Open+Sans:400italic,700italic,400,700' rel='stylesheet' type='text/css'>
<title>TEC-CEM | Modeling</title>
<title>TEC-CEM | Modeling</title>
<meta http-equiv="Content-Type" content="text/html; charset=utf-8">
<meta http-equiv="Content-Type" content="text/html; charset=utf-8">
Line 18: Line 19:
<body leftmargin="0" topmargin="0" marginwidth="0" marginheight="0">
<body leftmargin="0" topmargin="0" marginwidth="0" marginheight="0">
    
    
-
<map name="HeaderMAP">
 
-
    <area shape="rect" coords="3,2,78,72" href="https://2014.igem.org/">
 
-
    <area shape="rect" coords="82,4,157,74" href="http://www.itesm.mx/wps/wcm/connect/Campus/CEM/Estado+de+Mexico/">
 
-
    <area shape="rect" coords="158,4,234,72" href="#">
 
-
</map>
 
<table border="0" align="center" cellpadding="0" cellspacing="0" id="Table_01" style="background:transparent;">
<table border="0" align="center" cellpadding="0" cellspacing="0" id="Table_01" style="background:transparent;">
   <tr>
   <tr>
-
     <td colspan="3" valign="bottom"><h3> Biorremediación médica</h3></td>
+
     <td colspan="3" valign="bottom"><h3>ITESM-CEM | Enzy7-K me</h3></td>
-
     <td colspan="2" rowspan="2" align="right"><img src="https://static.igem.org/mediawiki/2014/9/9a/ITESMCEM_wiki_section_02.png" alt="" width="235" height="77" usemap="#HeaderMAP" border="0"></td>
+
     <td colspan="2" rowspan="2" align="right">
 +
 
 +
<div class="header">
 +
      <iframe src="https://2014.igem.org/Team:ITESM-CEM/HEADER" width="235" height="77" frameborder="0" scrolling="no" seamless></iframe>
 +
      </div>
 +
 
 +
</td>
     <td><img src="images/spacer.gif" width="1" height="43" alt=""></td>
     <td><img src="images/spacer.gif" width="1" height="43" alt=""></td>
   </tr>
   </tr>
Line 37: Line 39:
   <tr>
   <tr>
     <td rowspan="4">&nbsp;</td>
     <td rowspan="4">&nbsp;</td>
-
     <td rowspan="4" align="right" valign="top"><img src="https://static.igem.org/mediawiki/2014/1/13/ITESMCEM_wiki_section_05.png" alt="" width="165" height="34" usemap="#SocialMAP" border="0"></td>
+
     <td rowspan="4" align="right" valign="top">
-
   
+
 
-
    <map name="SocialMAP">
+
<div class="headerSocial">
-
    <area shape="rect" coords="-8,2,81,38" href="#">
+
    <iframe src="https://2014.igem.org/Team:ITESM-CEM/SOCIAL" width="165" height="34" frameborder="0" scrolling="no" seamless></iframe>    
-
    <area shape="rect" coords="82,2,161,34" href="#">
+
      </div>
-
</map>
+
      
      
     <td><img src="images/spacer.gif" width="1" height="6" alt=""></td>
     <td><img src="images/spacer.gif" width="1" height="6" alt=""></td>
Line 48: Line 49:
   <tr>
   <tr>
     <td colspan="3" rowspan="3" align="left" valign="top"><ul>
     <td colspan="3" rowspan="3" align="left" valign="top"><ul>
-
       <sub>Submenú 1</sub><sub>Submenú 2</sub><sub>Submenú 3</sub>
+
       <sub><a href="https://2014.igem.org/Team:ITESM-CEM/Modeling" style="color: #FFF;">Modeling Proteins</a></sub>
 +
      <sub><a href="https://2014.igem.org/Team:ITESM-CEM/CholesterolOxidase">Cholesterol Oxidase</a></sub>
 +
      <sub><a href="https://2014.igem.org/Team:ITESM-CEM/Oxoacyl">Oxoacyl Reductase</a></sub>
 +
      <sub><a href="https://2014.igem.org/Team:ITESM-CEM/Cholesterol">7-Dehydratase</a></sub>
 +
      <sub><a href="https://2014.igem.org/Team:ITESM-CEM/EnzymaticKinectics">Enzymatic Kinetics</a></sub>
     </ul></td>
     </ul></td>
-
     <td><img src="images/spacer.gif" width="1" height="25" alt=""></td>
+
     <td><img src="images/spacer.gif" width="1" height="1" alt=""></td>
   </tr>
   </tr>
   <tr>
   <tr>
Line 59: Line 64:
   </tr>
   </tr>
   <tr>
   <tr>
-
     <td colspan="5" valign="top"><h2>Subtítulo 1</h2>
+
     <td colspan="5" valign="top">
-
       <p>Lorem ipsum dolor sit amet, consectetur adipiscing elit. Curabitur id egestas ipsum. Curabitur non metus viverra, sollicitudin leo ut, rutrum nisl. In et consectetur sem, ut iaculis elit. In viverra tortor vitae sapien euismod bibendum. Etiam tincidunt lectus lectus, ut mollis tellus molestie faucibus. Interdum et malesuada fames ac ante ipsum primis in faucibus. Quisque et semper nunc. Vestibulum ante ipsum primis in faucibus orci luctus et ultrices posuere cubilia Curae; Nam porta diam nec sodales porta. Duis varius orci libero, in suscipit eros adipiscing ac. Phasellus condimentum ornare massa porttitor fermentum. Vestibulum ante ipsum primis in faucibus orci luctus et ultrices posuere cubilia Curae;</p>
+
 
-
      <p> Nulla porttitor aliquet elit, vel mollis sem malesuada sit amet. Cum sociis natoque penatibus et magnis dis parturient montes, nascetur ridiculus mus. Morbi vitae fermentum turpis. Suspendisse laoreet, elit quis pretium pharetra, est quam viverra ipsum, ac laoreet erat urna a odio. Quisque condimentum nibh erat, sed vulputate eros dictum vel. Cum sociis natoque penatibus et magnis dis parturient montes, nascetur ridiculus mus. Suspendisse viverra mauris sed adipiscing adipiscing. Proin egestas placerat sem, varius pretium est aliquam et. Etiam mattis mattis dolor, suscipit gravida lectus blandit a. Donec felis dui, venenatis lobortis ullamcorper at, consectetur et massa.</p>
+
<table width="100%" border="0" id="ContenidoSecciones">
-
      <h2>Subtítulo 2</h2>
+
<div style="background-color: #f3f3e2; style="width:95%">
-
      <p>Lorem ipsum dolor sit amet, consectetur adipiscing elit. Curabitur id egestas ipsum. Curabitur non metus viverra, sollicitudin leo ut, rutrum nisl. In et consectetur sem, ut iaculis elit. In viverra tortor vitae sapien euismod bibendum. Etiam tincidunt lectus lectus, ut mollis tellus molestie faucibus.</p>
+
<!--INICIO CONTENIDO-->
 +
 
 +
<h2>Modeling Proteins</h2>
 +
       <p style="text-align: justify; text-justify: inter-word">When studying a protein function it is insufficient to analyze its aminoacidic sequence. Although it provides very useful information most protein functions, if not all, relay on their tridimensional structure which requires a much more complex set of tools both in silico and experimental.
 +
For this project it is important to have tridimensional models of the enzymes mainly because they are not well known proteins with a detailed characterization nor do they have experimental tridimensional structures proposed in the available international databases (in fact one of them is classified as an hypothetical protein). Knowing the possible structure of these enzymes is very useful to compare them to other enzymes found to have a sequence and function homology and to further extent this homology with their structure, therefore assuring to a certain degree that the proteins expressed will work the way it is needed.</p><br>
 +
 
 +
<h2>Bioinformatic Tools</h2>
 +
    <h4>RCSB/PDB Database</h4>
 +
 
 +
<p style="text-align: justify; text-justify: inter-word"> This database provides a lot of useful information regarding the tridimensional structures of proteins as well as the relationships between the sequence, structure and function of proteins. For the project this database was mostly used to obtain the tridimensional models of comparable enzymes and to perform the sequence alignments as well as the homology analysis. </p><br>
 +
 
 +
<h4>I-Tasser</h4>
 +
<p style="text-align: justify; text-justify: inter-word"> This is an online platform that performs protein structure predictions based on the aminoacidic sequence. The end result of this platform are several tridimensional models each with a different probability of existance. The algorithims used by this platform allow it to deliver results in a matter of days. The 3D models of the project enzymes were obtained through I-Tasser.</p><br>
 +
 
 +
<h4>GOR VI & XTalPred-RF</h4>
 +
<p style="text-align: justify; text-justify: inter-word">Both of these sites are used toperform secondary structure predictions based on the aminoacidic sequence of a protein. XTalPred-RF in particular offers some other parameters regarding the stability and hydrophobicity of the resulting protein.</p><br>
 +
 
 +
<h4>Sequence Manipulation Suite</h4>
 +
<p style="text-align: justify; text-justify: inter-word">This site is a collection of programs for generating, formmating and analysing  DNA and protein sequences as their own website says. For the project it was used to translate DNA sequences to amino acidic sequences in fasta format.</p><br>
 +
 
 +
<h4>Pymol 2.7.7</h4>
 +
<p style="text-align: justify; text-justify: inter-word"> Pymol is a molecular visualization system which is free to use. This software allows the user to view and manipulate a tridimensional protein model . The user can select certain parts of the structure manually or using the aminoacidic sequence. Interactions between different molecules are also an object of great interest for molecular biology and with Pymol it is possible to analyse them and observe them. The 3D alignment and model analysis was made using Pymol as well as all the 3D images in this wiki. </p><br>
 +
 
 +
    <br><br><br>
 +
 
 +
<!--FIN CONTENIDO-->
 +
 
 +
</div>
 +
</table>
       <p>&nbsp;</p></td>
       <p>&nbsp;</p></td>
     <td><img src="images/spacer.gif" width="1" height="509" alt=""></td>
     <td><img src="images/spacer.gif" width="1" height="509" alt=""></td>
Line 82: Line 115:
       <td colspan="5" align="left" valign="bottom">
       <td colspan="5" align="left" valign="bottom">
       <div class="footer">
       <div class="footer">
-
       <img src="https://static.igem.org/mediawiki/2014/f/f8/ITESMCEM_wiki_section_12.png" width="240" height="82" alt=""></div>
+
       <iframe src="https://2014.igem.org/Team:ITESM-CEM/FOOTER" width="500" height="82" frameborder="0" scrolling="no" seamless></iframe></div>
       </td>
       </td>
       <td width="37%" align="right" valign="bottom">
       <td width="37%" align="right" valign="bottom">
        
        
-
       <div class="PLASMENU"> <img src="https://static.igem.org/mediawiki/2014/1/15/ITESMCEM_POPMENU.png" alt="" width="614" height="614" usemap="#PopmenuMAP" border="0">
+
       <div class="PLASMENU">  
-
        <map name="PopmenuMAP">
+
      <iframe src="https://2014.igem.org/Team:ITESM-CEM/POPMENU" width="614" height="614" frameborder="0" scrolling="no" seamless></iframe>
-
          <area shape="rect" coords="64,171,187,200" href="https://2014.igem.org/Team:ITESM-CEM/Project" alt="Project">
+
-
          <area shape="rect" coords="95,123,205,157" href="https://2014.igem.org/Team:ITESM-CEM/Parts" alt="Parts">
+
-
          <area shape="rect" coords="137,84,285,118" href="https://2014.igem.org/Team:ITESM-CEM/Modeling" alt="Modeling">
+
-
          <area shape="rect" coords="222,46,374,78" href="https://2014.igem.org/Team:ITESM-CEM/Notebook" alt="Notebook">
+
-
          <area shape="circle" coords="308,308,126" href="https://2014.igem.org/Team:ITESM-CEM" alt="Home">
+
-
          <area shape="poly" coords="89,98" href="#">
+
-
          <area shape="rect" coords="39,260,136,320" href="https://igem.org/Team.cgi?year=2014" alt="Official Team Profile">
+
-
          <area shape="rect" coords="35,342,138,372" href="https://2014.igem.org/Team:ITESM-CEM/Team" alt="Team">
+
-
          <area shape="rect" coords="83,422,178,467" href="https://2014.igem.org/Team:ITESM-CEM" alt="Home">
+
-
          <area shape="rect" coords="384,123,511,159" href="https://2014.igem.org/Team:ITESM-CEM/Safety" alt="Safety">
+
-
          <area shape="rect" coords="412,168,553,211" href="https://2014.igem.org/Team:ITESM-CEM/Attributions" alt="Attributions">
+
-
        </map>
+
       </div>
       </div>
        
        

Latest revision as of 01:36, 18 October 2014

TEC-CEM | Modeling

ITESM-CEM | Enzy7-K me

Modeling 3325

 

Modeling Proteins

When studying a protein function it is insufficient to analyze its aminoacidic sequence. Although it provides very useful information most protein functions, if not all, relay on their tridimensional structure which requires a much more complex set of tools both in silico and experimental. For this project it is important to have tridimensional models of the enzymes mainly because they are not well known proteins with a detailed characterization nor do they have experimental tridimensional structures proposed in the available international databases (in fact one of them is classified as an hypothetical protein). Knowing the possible structure of these enzymes is very useful to compare them to other enzymes found to have a sequence and function homology and to further extent this homology with their structure, therefore assuring to a certain degree that the proteins expressed will work the way it is needed.


Bioinformatic Tools

RCSB/PDB Database

This database provides a lot of useful information regarding the tridimensional structures of proteins as well as the relationships between the sequence, structure and function of proteins. For the project this database was mostly used to obtain the tridimensional models of comparable enzymes and to perform the sequence alignments as well as the homology analysis.


I-Tasser

This is an online platform that performs protein structure predictions based on the aminoacidic sequence. The end result of this platform are several tridimensional models each with a different probability of existance. The algorithims used by this platform allow it to deliver results in a matter of days. The 3D models of the project enzymes were obtained through I-Tasser.


GOR VI & XTalPred-RF

Both of these sites are used toperform secondary structure predictions based on the aminoacidic sequence of a protein. XTalPred-RF in particular offers some other parameters regarding the stability and hydrophobicity of the resulting protein.


Sequence Manipulation Suite

This site is a collection of programs for generating, formmating and analysing DNA and protein sequences as their own website says. For the project it was used to translate DNA sequences to amino acidic sequences in fasta format.


Pymol 2.7.7

Pymol is a molecular visualization system which is free to use. This software allows the user to view and manipulate a tridimensional protein model . The user can select certain parts of the structure manually or using the aminoacidic sequence. Interactions between different molecules are also an object of great interest for molecular biology and with Pymol it is possible to analyse them and observe them. The 3D alignment and model analysis was made using Pymol as well as all the 3D images in this wiki.