Team:ITESM-CEM/Attributions

From 2014.igem.org

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      <sub><a href="https://2014.igem.org/Team:ITESM-CEM/Interlab">Interlab</a></sub>
 
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      <sub><a href="https://2014.igem.org/Team:ITESM-CEM/Interlab">Existing GFP device</a></sub>
 
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      <sub><a href="https://2014.igem.org/Team:ITESM-CEM/Interlab">New GFP device 1</a></sub>
 
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      <sub><a href="https://2014.igem.org/Team:ITESM-CEM/Interlab">New GFP device 2</a></sub>
 
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<table width="100%" border="0" id="ContenidoSecciones">
<table width="100%" border="0" id="ContenidoSecciones">
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<h2> Attributions </h2>
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<h2>Overview</h2>
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<p style="text-align: justify; text-justify: inter-word;">The IGEM TEC CEM team has witnessed the effort during almost one and a half years since its foundation on July, 2013. Throughout all this time, until the delivery of results on October 17th; the 11 members that constitute this team, as well as the instructors and advisors, have shown the commitment and dedication possible towards the accomplishment of the main goal: medical bioremediation and a possible treatment for atherosclerosis. The work in the laboratory, institutional support and evaluation of the results would not have been possible without the following organizations and individuals dedicated to share their knowledge and support to the team:</p><br>
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      <p>During 2014 iGEM competition, teams were requested to analyse the efficiency of 3 different genetic devices (BioBricks) using GFP as a marker of gene expression. Here, iGEM ITESM CEM team presents the results of this interlab fluorescence measurement study.
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The three devices analysed are composed by a variable promoter, a gene encoding a mutant Green Fluorescence Protein (GFP) used as a marker of expression, and a plasmid backbone. Two promoters (BBa_J23101 and BBa_J23115, recently renamed BBa_K823005 and BBa_K823012 at iGEM’s catalogue) are used, both of them being members of a family of constitutive promoters described by Chris Anderson, member of iGEM Berkley Team, in 2006 (1). This family of parts is registered at the catalogue under the alphanumeric codes BBa_J23100 – BBa_J23119.
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<p style="text-align: justify; text-justify: inter-word;">
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Instituto Tecnológico y de Estudios Superiores de Monterrey Campus Estado de México (ITESM-CEM).<br>
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Two different plasmid backbones are used: a low-copy (psB3K3) and a high-copy plasmid (psB1C3). The GFP-expressing BioBrick remains the same for all devices (registered at the catalog as BBa_E0240), and is composed of a ribosome binding site (RBS), a mutant GFP gene, and two termination sequences.
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Department of Biotechnology Engineering and Chemical Engineering - ITESM CEM<br>
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<br><br>
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School of Design, Engineering and Architecture (EDIA) - ITESM CEM. <br>
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The aim of this study is to report the relative efficiency of the following genetic devices:<br><br>
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Center for Research and Advanced Studies of the National Polytechnic Institute (CINVESTAV-ZACATENCO).</p><br><br>
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1) Promoter BBa_K823005 in low-copy plasmid psB3K3<br>
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2) Promoter BBa_K823005 in high-copy plasmig psB1C3<br>
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<p style="text-align: justify; text-justify: inter-word;">Also, we would like to thank other people involved in this project and those who assured that the team reached every goal.</p><br><br>
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3) Promoter BBa_K823012 in high-copy plasmid psB1C3<br><br>
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In order to do so, GFP (BBa_E0240) is used as a marker of gene expression or reporter gene, because of the ease of fluorescence measurement experiments.<br><br>
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<p style="text-align: justify; text-justify: inter-word;">
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GFP has long been used as a reporter of patterns of gene expression in both prokaryotes, were it is useful for characterization of promoters, enhancers and terminators; and in eukaryotes, were tissue-specific or time-specific gene expression can be traced (2). The basis of this procedure is the usage of GFP’s fluorescence as a reporter of activity of promoters and enhancers; the relative fluorescence of cells at different experimental conditions can be compared with statistical techniques, and so the efficiency of the parts can be tested.
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Engineer Paola Mora Gazano, who was always on time to provide us all the necessary materials.<br>
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<br><br>
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M. in S. Carlos Cruz Cruz, who helped us make experimental procedures on the Interlab Measurement division and our project.<br>
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Heriberto Martínez and Melissa Escobar; who helped us in the protein analysis of our biobricks.<br>
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Ph. D. Juan Carlos Amador Molina who provided the necessary material for protein analysis.<br>
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Ph. D. Josefina Reyna Castillo, who gave us the necessary funds for the material needed to start working in the laboratory. <br>
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Biologist Ramón Rivero Aranda, who was always in the laboratory to help us with the equipment, to provide us material and to help us. <br>
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Ph. D. Berenice Vergara and Ph. D. Ricardo Swain, for their investment  in the team registration fee. <br>
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iGEM headquarters and Ana Sifuentes, for being there during the process, for attending our messages and giving us the necessary advices for our project. <br>
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Ollin Synbio, for helping us in diverse Human Practices as giving us funds for the fulfillment of them.<br>
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Mario Arturo Domínguez Ibarra, for helping the team with the planification and realization of Human Practices. <br>
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UANL, for their support in Interlab Measurement results and for helping the team with planification of Human Practices. <br>
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UNAM Genomics, for helping the team with the planification of Human Practices. <br>
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iGEM Biosint Mexico, for supporting the development of PCR protocols. <br>
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iGEM Tec-Monterrey, for their support with the results of Device 3 in Interlab. <br>
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iGEM Team Technische Universität Braunschweig, for supporting the team with the design and analysis of protocols. <br>
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Guillaume Mercy and iGEM NCTU Formosa team, for their support with the design of primers and giving us advice of the best conditions for initial PCRs. <br>
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High School volunteers, for helping us maintain the laboratory clean and in perfect conditions.<br>
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To all the people who attended Human Practices and made each event possible. <br>
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Agustín Miranda and Rodrigo Anguiano, for being part of the team and helping us in the beginning of this project. <br>
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To all our teachers, who gave us absolute support.<br>
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Our sponsors New England Biolabs and DANFREE for believing in us, despite being the first iGEM TEC CEM team.<br>
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Our beloved families and friends that were always supporting us no matter what. <br><br>
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Thanks to our advisors and instructors for the patience and support during the project. We’ve learnt significant things that will help us along the way.<br><br>
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Thank you!<br><br>
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iGEM TEAM 2014<br><br>
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<br><br><br><br><br><br>
 
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</table>
</table>
       <p>&nbsp;</p></td>
       <p>&nbsp;</p></td>

Revision as of 23:23, 17 October 2014

TEC-CEM | Attributions

ITESM-CEM | Enzy7-K me

Attributions 4205

 

Attributions

The IGEM TEC CEM team has witnessed the effort during almost one and a half years since its foundation on July, 2013. Throughout all this time, until the delivery of results on October 17th; the 11 members that constitute this team, as well as the instructors and advisors, have shown the commitment and dedication possible towards the accomplishment of the main goal: medical bioremediation and a possible treatment for atherosclerosis. The work in the laboratory, institutional support and evaluation of the results would not have been possible without the following organizations and individuals dedicated to share their knowledge and support to the team:


Instituto Tecnológico y de Estudios Superiores de Monterrey Campus Estado de México (ITESM-CEM).
Department of Biotechnology Engineering and Chemical Engineering - ITESM CEM
School of Design, Engineering and Architecture (EDIA) - ITESM CEM.
Center for Research and Advanced Studies of the National Polytechnic Institute (CINVESTAV-ZACATENCO).



Also, we would like to thank other people involved in this project and those who assured that the team reached every goal.



Engineer Paola Mora Gazano, who was always on time to provide us all the necessary materials.
M. in S. Carlos Cruz Cruz, who helped us make experimental procedures on the Interlab Measurement division and our project.
Heriberto Martínez and Melissa Escobar; who helped us in the protein analysis of our biobricks.
Ph. D. Juan Carlos Amador Molina who provided the necessary material for protein analysis.
Ph. D. Josefina Reyna Castillo, who gave us the necessary funds for the material needed to start working in the laboratory.
Biologist Ramón Rivero Aranda, who was always in the laboratory to help us with the equipment, to provide us material and to help us.
Ph. D. Berenice Vergara and Ph. D. Ricardo Swain, for their investment in the team registration fee.
iGEM headquarters and Ana Sifuentes, for being there during the process, for attending our messages and giving us the necessary advices for our project.
Ollin Synbio, for helping us in diverse Human Practices as giving us funds for the fulfillment of them.
Mario Arturo Domínguez Ibarra, for helping the team with the planification and realization of Human Practices.
UANL, for their support in Interlab Measurement results and for helping the team with planification of Human Practices.
UNAM Genomics, for helping the team with the planification of Human Practices.
iGEM Biosint Mexico, for supporting the development of PCR protocols.
iGEM Tec-Monterrey, for their support with the results of Device 3 in Interlab.
iGEM Team Technische Universität Braunschweig, for supporting the team with the design and analysis of protocols.
Guillaume Mercy and iGEM NCTU Formosa team, for their support with the design of primers and giving us advice of the best conditions for initial PCRs.
High School volunteers, for helping us maintain the laboratory clean and in perfect conditions.
To all the people who attended Human Practices and made each event possible.
Agustín Miranda and Rodrigo Anguiano, for being part of the team and helping us in the beginning of this project.
To all our teachers, who gave us absolute support.
Our sponsors New England Biolabs and DANFREE for believing in us, despite being the first iGEM TEC CEM team.
Our beloved families and friends that were always supporting us no matter what.

Thanks to our advisors and instructors for the patience and support during the project. We’ve learnt significant things that will help us along the way.

Thank you!

iGEM TEAM 2014