Team:ITB Indonesia/SelfMod

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<li>TEAM
<li>TEAM
<ul>
<ul>
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<li><a href="https://igem.org/Team.cgi?year=2014" >OFFICIAL TEAM PROFILE</a></li>
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<li><a href="https://igem.org/Team.cgi?id=1387" >OFFICIAL TEAM PROFILE</a></li>
<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Team">TEAM MEMBER</a></li>
<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Team">TEAM MEMBER</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/RepMod">REPORTER MODULE</a></li>
<li><a href="https://2014.igem.org/Team:ITB_Indonesia/RepMod">REPORTER MODULE</a></li>
<li><a href="https://2014.igem.org/Team:ITB_Indonesia/SelfMod">SELF REGULATORY MODULE</a></li>
<li><a href="https://2014.igem.org/Team:ITB_Indonesia/SelfMod">SELF REGULATORY MODULE</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/FutureSystem">FUTURE SYSTEM</a></li>
    </ul>
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<li>MODELING</li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Modeling">MODELING</a></li>
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<li>NOTEBOOK</li>
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<li>WETLAB
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/protocol">PROTOCOL</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Parts">PARTS</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Attributions">ATTRIBUTIONS</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Safety">SAFETY</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Data">DATA</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/ProteinModel">PROTEIN MODEL</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Achievement">ACHIEVEMENT</a></li>
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</li>
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<li>NOTEBOOK
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/nb-wetlab">WETLAB</a></li>
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<li>HUMAN PRACTICE
<li>HUMAN PRACTICE
<ul>
<ul>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/OnMedia">MEDIA</a></li>
<li><a href="https://2014.igem.org/Team:ITB_Indonesia/OnMedia">MEDIA</a></li>
<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Biosafety">BIOSAFETY SEMINAR</a></li>
<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Biosafety">BIOSAFETY SEMINAR</a></li>
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<li>ON PROGRESS</li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/MeetUp">INDONESIA TEAMS MEET UP</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Kids">FUN WITH KIDS</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/UPI">SHARING SYNBIO IN UPI</a></li>
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<br>
<h1>Self Regulatory Module</h1>
<h1>Self Regulatory Module</h1>
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<p>Self-regulatory module is designed to repair the inefficiency of system due to formation of inclusion body. Inclusion body is formed from aggregation of misfolded protein caused by overexpression of LC Cutinase protein (degradation module). Besides being inefficient to the system, inclusion body may also kill the organism because it gives a cytoplasmic stress to cell.</p>
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<p align="justify">Self-regulatory module is designed to repair the inefficiency of system due to formation of inclusion body. Inclusion body is formed from aggregation of misfolded protein caused by overexpression of LC Cutinase protein (degradation module). Besides being inefficient to the system, inclusion body may also kill the organism because it gives a cytoplasmic stress to cell.</p>
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<p>When inclusion body occurs, inclusion body inducible promoter (PibpAB) will active and the expression of TetR repressor will take place. This TetR repressor then corresponds to TetO in degradation module and inhibits the expression of LC Cutinase protein. This inhibition effect will eventually decreasing the inclusion body present in the cell and improving the system efficiency.</p>
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<p align="justify">When inclusion body occurs, inclusion body inducible promoter (PibpAB) will active and the expression of TetR repressor will take place. This TetR repressor then corresponds to TetO in degradation module and inhibits the expression of LC Cutinase protein. This inhibition effect will eventually decreasing the inclusion body present in the cell and improving the system efficiency.</p>
<img src="https://static.igem.org/mediawiki/2014/a/a6/Self-module.jpg">
<img src="https://static.igem.org/mediawiki/2014/a/a6/Self-module.jpg">

Latest revision as of 03:47, 17 October 2014


Self Regulatory Module

Self-regulatory module is designed to repair the inefficiency of system due to formation of inclusion body. Inclusion body is formed from aggregation of misfolded protein caused by overexpression of LC Cutinase protein (degradation module). Besides being inefficient to the system, inclusion body may also kill the organism because it gives a cytoplasmic stress to cell.

When inclusion body occurs, inclusion body inducible promoter (PibpAB) will active and the expression of TetR repressor will take place. This TetR repressor then corresponds to TetO in degradation module and inhibits the expression of LC Cutinase protein. This inhibition effect will eventually decreasing the inclusion body present in the cell and improving the system efficiency.