Team:ITB Indonesia/RepMod

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<li><a href="https://2014.igem.org/Team:ITB_Indonesia">HOME</a></li>
 
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<li>TEAM
 
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<li><a href="https://igem.org/Team.cgi?id=1387" >OFFICIAL TEAM PROFILE</a></li>
 
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Team">TEAM MEMBER</a></li>
 
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<li>PROJECT
 
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Project">OVERVIEW</a></li>
 
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/DegMod">DEGRADATION MODULE</a></li>
 
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/ConMod">CONVERTION MODULE</a></li>
 
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/RepMod">REPORTER MODULE</a></li>
 
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/SelfMod">SELF REGULATORY MODULE</a></li>
 
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/FutureSystem">FUTURE SYSTEM</a></li>
 
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Parts">PARTS</a></li>
 
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Attributions">ATTRIBUTIONS</a></li>
 
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Safety">SAFETY</a></li>
 
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Data">DATA</a></li>
 
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/ProteinModel">PROTEIN MODEL</a></li>
 
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Achievement">ACHIEVEMENT</a></li>
 
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Unpas">INTRO SYNBIO TO UNPAS</a></li>
 
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/SynBio">SYNBIO CLASS</a></li>
 
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/SynbiGreen">SYNBIGREEN SURVEY</a></li>
 
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Medsos">SOCIAL MEDIA</a></li>
 
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/OnMedia">MEDIA</a></li>
 
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Biosafety">BIOSAFETY SEMINAR</a></li>
 
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/MeetUp">INDONESIA TEAMS MEET UP</a></li>
 
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Kids">FUN WITH KIDS</a></li>
 
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/UPI">SHARING SYNBIO IN UPI</a></li>
 
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<h1>Reporter Module</h1>
 
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<p>Reporter module is designed to detect the existence of cytoplasmic stress by naked eye. One of the cytoplasmic stresses is the formation of inclusion body due to overexpression of LC Cutinase in degradation module. The overexpression of the protein may result in an inefficient protein folding where not 100% proteins are useable and probably, the misfolded protein will create an aggregate known as inclusion body that decreasing the system efficiency.</p>
 
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<p>Inclusion body inducible promoter (PibpAB) then will detect the formation of inclusion body and report it by expressing amilCP, a blue fluorescent protein that detectable by naked eyes, make it easier to analyze whether the stress level of the cell is in the limit or beyond the limit.</p>
 
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Latest revision as of 00:35, 18 October 2014


Reporter Module

Reporter module is designed to detect the existence of cytoplasmic stress by naked eye. One of the cytoplasmic stresses is the formation of inclusion body due to overexpression of LC Cutinase in degradation module. The overexpression of the protein may result in an inefficient protein folding where not 100% proteins are useable and probably, the misfolded protein will create an aggregate known as inclusion body that decreasing the system efficiency.

Inclusion body inducible promoter (PibpAB) then will detect the formation of inclusion body and report it by expressing amilCP, a blue fluorescent protein that detectable by naked eyes, make it easier to analyze whether the stress level of the cell is in the limit or beyond the limit.