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Team:ITB Indonesia/ConMod - Revision history
2024-03-29T13:43:58Z
Revision history for this page on the wiki
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Erawijantari at 03:14, 17 October 2014
2014-10-17T03:14:48Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h1>Convertion Module</h1></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h1>Convertion Module</h1></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <p>Convertion module is constructed to create an E.coli that able to survive the present of toxic agent, such as ethylene glycol that being introduce in the system. The module consist of two enzymes, glycoaldehyde reductase and glycoaldehyde dehydrogenase. The enzymes are responsible in metabolising ethylene glycol in E.coli strain BL21 (DE3). The first mentioned enzyme is responsible to catalyse convertion reaction of ethylene glycol to glycoaldehyde while the second one is responsible to catalyse the reaction of glycoaldehyde to glycolate. This intermediate will proceed the next step of metabolic pathway of E.coli and finally ends up on TCA cycle. This process will create an energy (ATP) that can be used by bacteria as carbon source, thus assuring us that ethylene glycol will not pollute the environment.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <p <ins class="diffchange diffchange-inline">align="justify"</ins>>Convertion module is constructed to create an <ins class="diffchange diffchange-inline"><i></ins>E.coli<ins class="diffchange diffchange-inline"></i> </ins>that able to survive the present of toxic agent, such as ethylene glycol that being introduce in the system. The module consist of two enzymes, glycoaldehyde reductase and glycoaldehyde dehydrogenase. The enzymes are responsible in metabolising ethylene glycol in <ins class="diffchange diffchange-inline"><i></ins>E.coli<ins class="diffchange diffchange-inline"></i> </ins>strain BL21 (DE3). The first mentioned enzyme is responsible to catalyse convertion reaction of ethylene glycol to glycoaldehyde while the second one is responsible to catalyse the reaction of glycoaldehyde to glycolate. This intermediate will proceed the next step of metabolic pathway of E.coli and finally ends up on TCA cycle. This process will create an energy (ATP) that can be used by bacteria as carbon source, thus assuring us that ethylene glycol will not pollute the environment.</p></div></td></tr>
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Erawijantari
http://2014.igem.org/wiki/index.php?title=Team:ITB_Indonesia/ConMod&diff=271487&oldid=prev
Mardikusumo at 12:16, 16 October 2014
2014-10-16T12:16:46Z
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Mardikusumo
http://2014.igem.org/wiki/index.php?title=Team:ITB_Indonesia/ConMod&diff=264714&oldid=prev
Mardikusumo at 06:02, 16 October 2014
2014-10-16T06:02:17Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href="https://2014.igem.org/Team:ITB_Indonesia/RepMod">REPORTER MODULE</a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href="https://2014.igem.org/Team:ITB_Indonesia/RepMod">REPORTER MODULE</a></li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href="https://2014.igem.org/Team:ITB_Indonesia/SelfMod">SELF REGULATORY MODULE</a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href="https://2014.igem.org/Team:ITB_Indonesia/SelfMod">SELF REGULATORY MODULE</a></li></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <li><a href="https://2014.igem.org/Team:ITB_Indonesia/FutureSystem">FUTURE SYSTEM</a></li></ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li>NOTEBOOK</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li>NOTEBOOK</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <li><a href="https://2014.igem.org/Team:ITB_Indonesia/nb-modeling">MODELING</a></li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href="https://2014.igem.org/Team:ITB_Indonesia/nb-wetlab">WETLAB</a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href="https://2014.igem.org/Team:ITB_Indonesia/nb-wetlab">WETLAB</a></li></div></td></tr>
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Mardikusumo
http://2014.igem.org/wiki/index.php?title=Team:ITB_Indonesia/ConMod&diff=236552&oldid=prev
Mardikusumo at 01:00, 15 October 2014
2014-10-15T01:00:17Z
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <li>MODELING</li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <li<ins class="diffchange diffchange-inline">><a href="https://2014.igem.org/Team:ITB_Indonesia/Modeling"</ins>>MODELING<ins class="diffchange diffchange-inline"></a></ins></li></div></td></tr>
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Mardikusumo
http://2014.igem.org/wiki/index.php?title=Team:ITB_Indonesia/ConMod&diff=236445&oldid=prev
Mardikusumo at 00:50, 15 October 2014
2014-10-15T00:50:51Z
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <li><a href="">PARTS</a></li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <li><a href="<ins class="diffchange diffchange-inline">https://2014.igem.org/Team:ITB_Indonesia/protocol">PROTOCOL</a></li></ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <li><a href="">ATTRIBUTIONS</a></li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li><a href="https://2014.igem.org/Team:ITB_Indonesia/Parts</ins>">PARTS</a></li></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <li><a href="<ins class="diffchange diffchange-inline">https://2014.igem.org/Team:ITB_Indonesia/Attributions</ins>">ATTRIBUTIONS</a></li></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <li><a href="">PROTEIN MODEL</a></li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <li><a href="<ins class="diffchange diffchange-inline">https://2014.igem.org/Team:ITB_Indonesia/ProteinModel</ins>">PROTEIN MODEL</a></li></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <li><a href="">ACHIEVEMENT</a></li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <li><a href="<ins class="diffchange diffchange-inline">https://2014.igem.org/Team:ITB_Indonesia/Achievement</ins>">ACHIEVEMENT</a></li></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href="https://2014.igem.org/Team:ITB_Indonesia/Biosafety">BIOSAFETY SEMINAR</a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href="https://2014.igem.org/Team:ITB_Indonesia/Biosafety">BIOSAFETY SEMINAR</a></li></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <li><a href="">INDONESIA TEAMS MEET UP</a></li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <li><a href="<ins class="diffchange diffchange-inline">https://2014.igem.org/Team:ITB_Indonesia/MeetUp</ins>">INDONESIA TEAMS MEET UP</a></li></div></td></tr>
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Mardikusumo
http://2014.igem.org/wiki/index.php?title=Team:ITB_Indonesia/ConMod&diff=232968&oldid=prev
Mardikusumo at 19:50, 14 October 2014
2014-10-14T19:50:49Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h1>Convertion Module</h1></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h1>Convertion Module</h1></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p>Convertion module is constructed to create an E.coli that able to survive the present of toxic agent, such as ethylene glycol that being introduce in the system. The module consist of two enzymes, glycoaldehyde reductase and glycoaldehyde dehydrogenase. The enzymes are responsible in metabolising ethylene glycol in E.coli strain BL21 (DE3). The first mentioned enzyme is responsible to catalyse convertion reaction of ethylene glycol to glycoaldehyde while the second one is responsible to catalyse the reaction of glycoaldehyde to glycolate. This intermediate will proceed the next step of metabolic pathway of E.coli and finally ends up on TCA cycle. This process will create an energy (ATP) that can be used by bacteria as carbon source, thus assuring us that ethylene glycol will not pollute the environment.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p>Convertion module is constructed to create an E.coli that able to survive the present of toxic agent, such as ethylene glycol that being introduce in the system. The module consist of two enzymes, glycoaldehyde reductase and glycoaldehyde dehydrogenase. The enzymes are responsible in metabolising ethylene glycol in E.coli strain BL21 (DE3). The first mentioned enzyme is responsible to catalyse convertion reaction of ethylene glycol to glycoaldehyde while the second one is responsible to catalyse the reaction of glycoaldehyde to glycolate. This intermediate will proceed the next step of metabolic pathway of E.coli and finally ends up on TCA cycle. This process will create an energy (ATP) that can be used by bacteria as carbon source, thus assuring us that ethylene glycol will not pollute the environment.</p></div></td></tr>
</table>
Mardikusumo
http://2014.igem.org/wiki/index.php?title=Team:ITB_Indonesia/ConMod&diff=232961&oldid=prev
Mardikusumo at 19:49, 14 October 2014
2014-10-14T19:49:43Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li>TEAM</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li>TEAM</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <ul></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <li><a href="https://igem.org/Team.cgi?<del class="diffchange diffchange-inline">year</del>=<del class="diffchange diffchange-inline">2014</del>" >OFFICIAL TEAM PROFILE</a></li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <li><a href="https://igem.org/Team.cgi?<ins class="diffchange diffchange-inline">id</ins>=<ins class="diffchange diffchange-inline">1387</ins>" >OFFICIAL TEAM PROFILE</a></li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href="https://2014.igem.org/Team:ITB_Indonesia/Team">TEAM MEMBER</a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href="https://2014.igem.org/Team:ITB_Indonesia/Team">TEAM MEMBER</a></li></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li>MODELING</li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li>MODELING</li></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <li>NOTEBOOK</li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li>WETLAB</ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li><a href="">PARTS</a></li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li><a href="">ATTRIBUTIONS</a></li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li><a href="https://2014.igem.org/Team:ITB_Indonesia/Safety">SAFETY</a></li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li><a href="">DATA</a></li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li><a href="">PROTEIN MODEL</a></li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li><a href="">ACHIEVEMENT</a></li></ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href="https://2014.igem.org/Team:ITB_Indonesia/OnMedia">MEDIA</a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href="https://2014.igem.org/Team:ITB_Indonesia/OnMedia">MEDIA</a></li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href="https://2014.igem.org/Team:ITB_Indonesia/Biosafety">BIOSAFETY SEMINAR</a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href="https://2014.igem.org/Team:ITB_Indonesia/Biosafety">BIOSAFETY SEMINAR</a></li></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <li><del class="diffchange diffchange-inline">ON PROGRESS</del></li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <li><ins class="diffchange diffchange-inline"><a href="">INDONESIA TEAMS MEET UP</a></li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li><a href="">FUN WITH KIDS</a></li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li><a href="">SHARING SYNBIO IN UPI</a></ins></li></div></td></tr>
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Mardikusumo
http://2014.igem.org/wiki/index.php?title=Team:ITB_Indonesia/ConMod&diff=209217&oldid=prev
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2014-10-13T03:07:49Z
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia">HOME</a></li><br />
<li>TEAM<br />
<ul><br />
<li><a href="https://igem.org/Team.cgi?year=2014" >OFFICIAL TEAM PROFILE</a></li><br />
<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Team">TEAM MEMBER</a></li><br />
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<li>PROJECT<br />
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Project">OVERVIEW</a></li><br />
<li><a href="https://2014.igem.org/Team:ITB_Indonesia/DegMod">DEGRADATION MODULE</a></li><br />
<li><a href="https://2014.igem.org/Team:ITB_Indonesia/ConMod">CONVERTION MODULE</a></li><br />
<li><a href="https://2014.igem.org/Team:ITB_Indonesia/RepMod">REPORTER MODULE</a></li><br />
<li><a href="https://2014.igem.org/Team:ITB_Indonesia/SelfMod">SELF REGULATORY MODULE</a></li><br />
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<li>MODELING</li><br />
<li>NOTEBOOK</li><br />
<li>HUMAN PRACTICE<br />
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Unpas">INTRO SYNBIO TO UNPAS</a></li><br />
<li><a href="https://2014.igem.org/Team:ITB_Indonesia/SynBio">SYNBIO CLASS</a></li><br />
<li><a href="https://2014.igem.org/Team:ITB_Indonesia/SynbiGreen">SYNBIGREEN SURVEY</a></li><br />
<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Medsos">SOCIAL MEDIA</a></li><br />
<li><a href="https://2014.igem.org/Team:ITB_Indonesia/OnMedia">MEDIA</a></li><br />
<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Biosafety">BIOSAFETY SEMINAR</a></li><br />
<li>ON PROGRESS</li><br />
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<h1>Convertion Module</h1><br />
<p>Convertion module is constructed to create an E.coli that able to survive the present of toxic agent, such as ethylene glycol that being introduce in the system. The module consist of two enzymes, glycoaldehyde reductase and glycoaldehyde dehydrogenase. The enzymes are responsible in metabolising ethylene glycol in E.coli strain BL21 (DE3). The first mentioned enzyme is responsible to catalyse convertion reaction of ethylene glycol to glycoaldehyde while the second one is responsible to catalyse the reaction of glycoaldehyde to glycolate. This intermediate will proceed the next step of metabolic pathway of E.coli and finally ends up on TCA cycle. This process will create an energy (ATP) that can be used by bacteria as carbon source, thus assuring us that ethylene glycol will not pollute the environment.</p><br />
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<img src="https://static.igem.org/mediawiki/2014/9/9a/Convertion-module.jpg"><br />
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