• 

• PROJECT

  • 
    PROJECT
  • PROJECT SUMMARY
  • ACHIEVEMENTS

• WETLAB

  • 
    WETLAB
  • WETLAB SUMMARY
  • RESULTS
  • NOTEBOOK
  • PROTOCOLS
  • DATA PAGE

• MODELING

  • 
    MODELING
  • MODELING SUMMARY
  • MOLECULAR MODELISATION
  • CURLI SYNTHESIS
•   HUMAN PRACTICE
  • 
    HUMAN
    PRACTICE
  • HUMAN PRACTICE SUMMARY
  • SYNBIO PERCEPTION
  • PROJECT'S INTEREST

• TEAM

  • 
    TEAM
  • MEMBERS & ATTRIBUTIONS
  • SPONSORS
  • PARTNERSHIPS
  • HAVE FUN WITH IGEM
  • GALLERY

MEMBERS & ATTRIBUTIONS

Figure 3: Preparing the rack with 50 empty capsules.

• STUDENTS

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• Alexia Vidal (INSA)
  
  
  

  Undergraduate student in Biochemistry and Biotechnology at INSA Lyon, I provided support for the 2013 European Jamboree organization. This experience has really motivated me to participate at the 2014 iGEM adventure! I’ve loved working this summer with our bacteria but especially with all the INSA Lyon team members. Moreover, I’ve been in charge of the project budget management. Outside the school, I enjoy scuba-diving, playing the flute in a harmony orchestra, running, playing golf, going out with friends and travelling. I look forward to meeting you all in Boston!
  
  
  

• Romain Tching Chi Yen (INSA)
  
  
  

  Now fourth year student in modelisation and bio-computing, I'm the waffle master of the team. Though not the most efficient, I'm dedicated to my work and spent and incredible summer with everyone working on this project.
  My contribution to the team was mainly with the mathematical model (no, I swear I'm nice, don't run away!) and human practice work, but I also helped in the wetlab for the clonig period, as well as whenever help was needed, be it for an experiment, making solutions, washing instruments, filling pipette tips boxes, etc. I could do everything. Kind of like a super hero...
  Yes, you may refer to me as WaffleMan!

  
  
  
  
• Africa Smith de Diego (INSA)
  
  
  

  I am a 5th year student of Biochemistry and Biotechnologies at INSA Lyon, graduating in a few months. I decided to join INSA Lyon iGEM team in order to have a look at what research is and work in a biotechnology project and it was way better than expected! This experience has shown me the wonders and the possibilities of synthetic bio and has motivated me to be a researcher in the future.

  During the summer, I was specially in charge of the cloning period, characterization tests and the final experiences on nickel chelation. Also, I participated in the iGEM France meet up and had a great time with the other iGEMers!

  In my free time, I like travelling, going to concerts, dance and practice sports.

  
  
  
  
• Adam Amara (Lyon 1)
  
  
  

  Final year student in BSc of Biochemistry at Université Claude Bernard Lyon 1. I am interested in Synthetic Biology and the possibility to solve current problems in our society. The iGEM seems to be the best way to discover the world of synthetic biology, and getting involved in a great project with real solutions and a real impact like the project Curly'on.
  I have been in charge of the Nickel tests, but I was also available to help my mates in the lab for other tasks, for the iGEM you must be MULTI-TASK !
  I'm passioned by technologies, open science, DIY, rowing, cinema and Science Fiction (Dr Who!).

  
  
  
  
• Alice Le Boedec (INSA)
  
  
  

  Based on my interest for biology as undergraduate student in Biochemistry and Biotechnologies at INSA Lyon, I started putting a lot into the school association “DNA Concept Club”. I was in charge of communication and my goal was to promote synthetic biology. Then I heard about the IGEM competition and I felt naturally attracted! Let's talk about this amazing experience... Surnamed “miss bacteria killer”, I was all the summer especially in charge of degrading biofilms and bacterian DNA before their use in the biofilter. For the human practice part, I organized visits at the purification station and the dialysis center. Liking art in general, I was pleased to write some lyrics for our clip Plasmidoutai as well as to be the photographer of the team ! One last point : I took part in the French IGEM meetup, from which I have a lot of good memories !

  
  
  
  
• Benjamin Rémy (INSA)
  
  
  

  I am a fifth year in biochemistry and biotechnology but must of all I am passionate by biology and all its applications. People talks a lot of the progress on phones or computer but It is really amazing what now we can do with just bacteria and designed DNA molecules. That why I have done the iGEM. Furthermore, the iGEM kicks you out of your classic lab work to give you the opportunity to design your own experiment. Indeed, during the summer, my big job was to search, adapt, work on and sometimes create protocols for the characterisation our modified curli and also for its chelating power. That makes the iGEM a really funny and creative experience. Finally, the best part, the summer lab work allow me to dance and sing during the experiments...Oh that explain we had a rainy summer !!

  
  
  
  
• Chloé Marquet--Jouve (INSA)
  
  
  

  I am a fourth year student at INSA de Lyon, in the biochemistry and biotechnologies department. I discovered the iGEM competition last year, during the European Jamboree in Lyon and I immediately adhered to the concept, for the scientific aspect as well as the human one. I wanted to join the iGEM team in order to work in a pluridisciplinary environment on an project which is combining biology and technology into a product.
  First of all, I have loved working with all of my fellows. I’ve learnt a lot from each of them, and enjoy all these moments spent together, during this summer : cheering each other in the bad times as partying in the good ones.
  During my leisure time, I play soccer on the INSA team, that’s why I’ve particularly enjoyed our cloning-and-world-cup nights :)

  
  
  
  
• Yang Zhang (INSA)
  
  
  

  Typical Chinese guy with a super power of mental calculation. Don't like to sing or any kind of physical activities. That's all you have to know.

  
  
  
  
• Juliette Paillet (INSA)
  
  
  

  I am a 4th year student in Biochemistry and biotechnologies at INSA Lyon. I was a volunteer for the organization of the European Jamboree in Lyon in September 2013, and this made me want to participate to the iGEM competition as a student. I started my iGEM activities with the association “INSA AND Concept Club” and was in charge of the administrative side of the project. In the lab I participated in cloning and curli characterization. That was a great new experience and very educational. I loved working with all the team during the summer and had a lot of fun !
  Except my passion for synthetic biology ;) I also like drawing and running. I am very exiting about this Giant Jamboree and can’t wait to meet all the iGEMers.

  
  
  
  
• Madalina Cercel (INSA)
  
  
  

  Being a volunteer in the 2013 European Jamboree gave me the opportunity to discover the iGEM Competition and the motivation to be part of this year’s team. Although a 4th year student at INSA Lyon in Bioinformatics and Modeling, I spent all the summer in the lab. And I loved every single moment of it! My work consisted especially in developing protocols, test and improve them, but I took part, as the rest of us, in the long late cloning hours. This project has been a great experience for me. It allowed me to get closer to the research field and showed me that this is the path I want to follow in the future. I spend my free time doing all kind of sports: skiing, playing tennis, biking, but I also enjoy travelling, reading and watching movies.

  
  
  
  
• Thomas Cruzel(EMSE)
  
  
  

  Student at the "École des mines de Saint-Étienne" school, the iGEM project was an opportunity for me to discover team work. As i'm more interested in computers than in biology, during this project i work mainly on modelisation of curli formation and nickel chelation.

  
  
  
  
• Alice Blot (DSAA Design Interactif, Villefontaine)
  
  
  

  I am studying interactive design in Master degree and the link between design and science captivates me. I am passionate by these two fields. I had to make a choice…. and I chose Design. Altought, as soon as possible, I combine them.
  I know the iGEM thanks to Juliette. She asked me to design their wiki. I accepted immediatly ! Even tough I don’t understand very well fine details, I note curly cells are great !

  
  
  
  

• ADVISORS

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• Nicolas Comte (INSA)
  
  
  

  RRAAAAAAAAWWwwwrr! I'm a dinosaur!

  
  
  
  
• Pedro Da Silva (INSA)
  
  
  

  Associate professor at the INSA de LYON institute, i'm a structural biologist. I think that iGEM is an exciting way to introduce research and team work to students.

  
  
  
  
• Damien Jacques (INSA)
  
  
  

  I am a mechanical design and production teacher at INSA Lyon. I am specially interested in 3D Printers, that is the raison why I was asked to participate to the IGEM project. I agreed with pleasure because I like working whith students on such interdisciplinary projects. I think it is important that students know 3D printers technologies because 3D Printers will provide many opportunities in several domains in the next 10 or 20 years and the IGEM project is an exemple for this. Otherwise I like to play tennis, to ski and to walk in mountain, and also to cook french food with my family and my friends !

  
  
  
  
• Benoît Drogue (Lyon 1 Alumnus)
  
  
  

  Young Microbiologist, I'm a dreamer with a scientific heart... everything you need to succeed in iGEM competition.

  
  
  
  
• Viviane Chansavang (INSA Alumna)
  
  
  

  iGEM 2011, 2012, 2013 and... 2014? I guess we can call it dedication...or is it addiction?
  I graduated from INSA de Lyon in September 2013, worked hard in the organizing committee of the Europe Regional Jamboree in October 2013, and then traveled across the planet to sunny Southern California for my first job. Although I traded in the bench for a desk, I'm still showing support to my home team. Working at the Office for Science and Technology of the Embassy of France in the US, I am striving at fostering partnerships between French and American labs in the field of life sciences.

  
  
  
  
• Valérie Forest (EMSE)
  
  
  

  Graduated in Biologic and Medical Engineering, I am assistant Professor at Ecole Nationale Supérieure des Mines de Saint-Etienne. I especially work in the field of nanotechnologies, but our engineering school is also highly interested in the development of synthetic biology and strongly believes in its outstanding applications. The iGEM competition gives us the opportunity to work in close collaboration with other regional and international institutions in a very interdisciplinary field. This exceptional experience is highly interesting, motivating and rewarding for students and advisors both on scientific and human aspects.

  
  
  
  

• INSTRUCTORS

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• Yoann Louis (INSA)
  
  
  

  Associate professor at the Institut National des Sciences Appliquées de Lyon and coordinator of the 2014 iGEM INSA-LYON Team.
  Up to now my work is mainly to study trace metals / organic matter behavior in various environment and to give an expertise on their potential toxicity depending on the bio-physico-chemical conditions of the studied site. I'm also now working on metal and biological material interaction in order to found new way to treat human waste.
  As a chemist, my interest in the iGEM project is to have an interdisciplinary approach of the project and not only a biological approach.

  
  
  
  
• Corinne Dorel-Flamant (INSA)
  
  
  

  My teaching activities in the Microbial Genetics mainly take place within the Biosciences department at INSA Lyon, but also at the Ecole Normale Supérieur de Lyon and at the University Claude Bernard Lyon 1.
  My research work is based on the understanding of genetic mechanisms involved in the formation of biofilms and the contamination of materials. Being the Communications officer for the Biosciences department, my participation in iGEM is part of a strategy to promote and share knowledge in the field of research in genetic engineering and more generally in Biological Sciences.

  
  
  
  
• Agnès Rodrigue (INSA)
  
  
  

  As an associate professor I find that iGEM is an unique experience, a place to mix teaching and practice in a long term project relying on students’ motivation. My teaching interests are microbiology, molecular biology, protein engineering and bioinformatics.
  My research topic focus on understanding the molecular mechanisms involved in bacterial adaptation to a metal-rich environment, from molecular interactions to population biology. Apart from the basic approaches, this subject offers also the opportunity to develop biological tools for the bioremediation of spoiled environment or the in situ detection of toxic compounds for instance, developments which I’m interested in. My motivation for iGEM is the same: conception and application.

  
  
  
  
• Marie-Pierre Escudié (INSA)
  
  
  

  PhD in Political Science ("Gaston Berger, les sciences humaines et les sciences de l'ingénieur, un projet de réforme de la société", Université Lumière Lyon 2, December 2013). I'm presently a Research engineer at INSA Lyon, i studie how politics, culture, and ethics of engineer shape science and technology.

  
  
  
  
• Marianne Chouteau (INSA)
  
  
  

  Marianne Chouteau has a PhD in Communication Science ("Les intentions vulgarisatrices : études d'ouvrage de vulgarisation scientifique de 1686 aux années 1950", Université Paris 7, Ed du Septentrion, 2000) . Researcher and professor in Humanities Center at INSA de Lyon, she studies the representation of science and technology in communication such as "cop and lab" series (CSI or Bones), movies, novels...

  
  
  
  
• Valérie Desjardin (INSA)
  
  
  

  Associate professor at the Institut National des Sciences Appliquées de Lyon My research work at the Laboratory of Civil engineering and Environmental engineering (LGCIE) is currently aimed at the study of biophysicochemical interactions of pollutants in various compounds (soils, sediments, municipal solid waste) using molecular biology tools. I am very excited to take part in the iGEM project which leads to the development of a synergy, already launched, between the Biosciences department and Environmental sciences.

  
  
  
  
• Alexandre Duprey (Lyon 1)
  
  
  
  

  PhD student at Université Claude Bernard Lyon 1, I'm back for a fourth year of iGEM ! This time as an instructor, I'm excited to see entirely new aspects of an iGEM project, and experimenting teaching in an unusual and rather unique way.
  Apart from iGEM, I'm preparing a thesis on the regulation of the virulence of the phytopathogen Dickeya dadantii. You know, complex stuff involving 5 different proteins, 2 promoters and DNA supercoiling acting simultaneously on a single gene... Still, I hope my experience with genetic regulation, reporters and data analysis prove useful to the team.

  
  
  
  
• Emiel van der Kouwe (Pharmacist and biologist)
  
  
  

  Pharmacist and graduated in Systems and Synthetic Biology, my first experience with the iGEM competition was in Evry (2013) where we suggested using iron-chelating bacteria as an alternative treatment to hemochromatosis. The experience was intense and stressful, albeit very addictive. Since I'm back in my hometown, I'm doing this all over again but as a supervisor at the INSA-Lyon. I'm having a whole new experience in this competition. So far, my favorite experience during this summer was to relentlessly tease Juliette Paillet and test her mental toughness. Secretly, I still pray for the Lord Iron Frog, our Savior and Salvation who daily bears our burdens.

  
  
  
  

• ATTRIBUTIONS

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• Who did what ?

  
  

  Birth of the project, brainstorming

  
  The entire team with the help and advice of the instructors.
  
  

  (Big and Heavy) Administrative Task

  
  Students : Juliette Paillet, Alexia Vidal
  Instructors : Yoann LOUIS, Corinne DOREL
  
  

  Sponsoring

  
  Students : Juliette Paillet, Alexia VIDAL
  Instructors : Yoann LOUIS, Corinne DOREL, Agnès RODRIGUE
  Advisors : Viviane CHANSAVANG
  
  

  Communication

  
  Students : Alice LE BOEDEC
  Instructors : Corinne DOREL, Yoann LOUIS
  Advisors : Benoit DROGUE, Nicolas COMTE
  
  

  Parts design

  
  Students : Yang ZHANG, Juliette PAILLET
  Instructors : Alexandre DUPREY, Corinne DOREL, Agnès RODRIGUE
  
  

  Lab management

  
  Students : Benjamin REMY, Romain TCHING CHI YEN
  Instructors : Corinne DOREL, Yoann LOUIS, Agnès RODRIGUE
  
  

  Labwork

  
  Students : Madalina CERCEL, Africa SMITH DE DIEGO, Yang ZHANG, Romain TCHING CHI YEN, Juliette PAILLET, Alexia VIDAL, Chloé MARQUET--JOUVE
  Instructors : Corinne DOREL, Agnès RODRIGUE, Alexandre DUPREY, Emiel VAN DER KOUWE
  
  

  Confocal laser microscopy, Transmission Electron Microscopie (TEM) in Lyon

  
  Students : Alexia VIDAL
  Instructors : Corinne DOREL, Yoann LOUIS
  Other: With the Help of Béatrice BURDIN of the "Centre Technologique des Microstructures, Lyon 1"
  
  

  Nickel Chelation

  
  Students : Chloé MARQUET--JOUVE, Adam AMARA, Africa SMITH DE DIEGO, Yang ZHANG, Juliette PAILLET
  Instructors : Yoann LOUIS, Corinne DOREL, Agnès RODRIGUE
  Other : With the Help of Florent ARNAUD-GODET of the ENS de LYON for ICP-MS analysis
  
  

  Modeling

  
  Students : Romain TCHING CHI YEN, Thomas CRUZEL
  Instructors : Agnès RODRIGUE, Emiel VAN DER KOUWE
  Advisors : Nicolas COMTE, Pedro DA SILVA
  
  

  Collaboration - 3D printing

  
  Students : Alice LE BOEDEC, Romain TCHING CHI YEN, Chloé MARQUET--JOUVE
  Instructors : Yoann LOUIS
  Advisors : Damien JACQUES, Benoit DROGUE, Valérie FOREST
  
  

  Human practice

  
  Students : Alice LE BOEDEC, Chloé MARQUET--JOUVE, Juliette PAILLET, Romain TCHING CHI YEN, Yang ZHANG
  Instructors : Marie Pierre ESCUDIE, Marianne CHOUTEAU
  
  

  Safety and Bacteria Killing

  
  Students : Alice LE BOEDEC, Africa SMITH DE DIEGO, Benjamin REMY
  Instructors : Corinne DOREL, Yoann LOUIS
  Others : Special thanks to Bruno CEDAT for the UV-lamp lent
  
  

  Wiki

  
  - Design and coding :
  Students : Alice BLOT
  - Content :
  Students : Nearly all except some with curli hair in the hand ...
  Instructors : Yoann LOUIS, Corinne DOREL, Agnès RODRIGUE, Marie-Pierre ESCUDIE, Valérie DESJARDIN, Emiel VAN DER KOUWE
  Advisors : Nicolas COMTE
  
  

  Oral presentation

  
  Students : Africa SMITH DE DIEGO, Yang ZHANG, Alice LE BOEDEC, Madalina CERCEL
  Instructors : Valérie DESJARDIN, Corinne DOREL, Agnès RODRIGUE, Yoann LOUIS, Emiel VAN DER KOUWE
  Other : a production of Davyd Chaumard (INSA Lyon), and participation of Anne HAZIZA (Amazing Alumnu iGEM speaker!)
  
  

  Poster

  
  Students : Africa SMITH DE DIEGO, Alice LE BOEDEC
  Instructors : Valérie DESJARDIN, Emiel VAN DER KOUWE
  Advisors : Nicolas COMTE
  
  

  "Cobalt buster" continuation

  
  As a side project, 2 parts related to the 2011 "Cobalt buster" project have been characterised, deposited in the registry and published in a peer-reviewed journal ( K1404001 and K1404002).
  Instructors : Alexandre DUPREY, Yoann LOUIS, Valérie DESJARDIN, Agnès RODRIGUE, Corinne DOREL
  Advisors : Viviane CHANSAVANG
  Other : Franck FREMION, Clémence GONTHIER, Philippe LEJEUNE, Fanny SPRINGER