Team:INSA-Lyon/Results

From 2014.igem.org

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   <td><div align="justify"><figcaption> PCR gel after UV time exposure </figcaption></td></div>
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   <td><div align="justify"><figcaption> PCR gel after UV time exposure </figcaption></div></td>
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</table> <br/> Bacterian DNA seemed to be degraded after 10 min UV light exposure.<br/>&rArr; <b>In consequence, UV light can be used to destroy DNA.</b> </p><br/>
</table> <br/> Bacterian DNA seemed to be degraded after 10 min UV light exposure.<br/>&rArr; <b>In consequence, UV light can be used to destroy DNA.</b> </p><br/>
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   <td><div align="center"><img src="https://static.igem.org/mediawiki/2014/3/3c/Gel_PCR_70.png" alt="control plate" width="200 px"/></td></div>
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   <td><div align="center"><img src="https://static.igem.org/mediawiki/2014/3/3c/Gel_PCR_70.png" alt="PCR gel" width="200 px"/></td></div>
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</table></br> No difference of coloration was observed between the control and the samples heated at 70°C : indeed a lot of green-colored bacteria remained after 45 min of heating.<br/>&rArr; <b>Temperature isn’t enough to kill bacteria just like UV light.</b></p><br/>
</table></br> No difference of coloration was observed between the control and the samples heated at 70°C : indeed a lot of green-colored bacteria remained after 45 min of heating.<br/>&rArr; <b>Temperature isn’t enough to kill bacteria just like UV light.</b></p><br/>
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<p> To solve this last problem, bacteria were put in contact with ethanol absolute. The Back Light coloration gives the following picture. <br/> image benjamin</p><br/><br/>
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<p> To solve this last problem, bacteria were put in contact with ethanol absolute. The Back Light coloration gives the following picture. <br/>  
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  <td><div align="center"><img src="https://static.igem.org/mediawiki/2014/2/2a/Image_benjamin.png" alt="back light ethanol" width="600 px" /></td></div>
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  <td><div align="center">Backlight after DNA extraction of bacterian culture exposed to ethanol<figcaption></figcaption></td></div>
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<p>These numerous experiments lead us to developp a protocol in three steps, illustrated by the drawing below :<br/>
<p>These numerous experiments lead us to developp a protocol in three steps, illustrated by the drawing below :<br/>

Revision as of 23:48, 17 October 2014

Curly'on - IGEM 2014 INSA-LYON

  • Curli characterization


  • Nickel chelation


  • Survival after UV and high temperature exposure


  • Promoter optimization and characterization