Team:INSA-Lyon/Results

From 2014.igem.org

(Difference between revisions)
(Undo revision 364238 by Alex273 (talk))
Line 37: Line 37:
<h5>Adhesion test and curli production</h5>
<h5>Adhesion test and curli production</h5>
<br/>
<br/>
-
<div align=”center”><img src="https://static.igem.org/mediawiki/2014/0/0a/Adh%C3%A9rence006.png" alt="Figure 1 : Engineered bacteria Percentage of adhesion"/></div>
+
<div align=”center”><img src="https://static.igem.org/mediawiki/2014/0/0e/Adh%C3%A9rence.png" alt="Figure 1 : Engineered bacteria Percentage of adhesion"/></div>
<b>Figure 1 : Engineered bacteria Percentage of adhesion</b><br/>
<b>Figure 1 : Engineered bacteria Percentage of adhesion</b><br/>
<p align="justify"><i>csgA-</i>knockout <i>E. coli</i> strain was transformed with BBa_CsgA-WT (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1404006">BBa_K1404006</a>); BBa_CsgA-His1 (<a href="http://parts.igem.org/Part:BBa_K1404007">BBa_K1404007</a>); BBa_CsgA-His2 (<a href="http://parts.igem.org/Part:BBa_K1404008">BBa_K1404008</a>). The corresponding positive and negative controls are Wild-type <i>E.coli</i> curli producing strain transformed with with the empty vector and <i>csgA-</i>-knockout <i>E. coli</i> strain transformed with the empty vector respectively. <br/>
<p align="justify"><i>csgA-</i>knockout <i>E. coli</i> strain was transformed with BBa_CsgA-WT (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1404006">BBa_K1404006</a>); BBa_CsgA-His1 (<a href="http://parts.igem.org/Part:BBa_K1404007">BBa_K1404007</a>); BBa_CsgA-His2 (<a href="http://parts.igem.org/Part:BBa_K1404008">BBa_K1404008</a>). The corresponding positive and negative controls are Wild-type <i>E.coli</i> curli producing strain transformed with with the empty vector and <i>csgA-</i>-knockout <i>E. coli</i> strain transformed with the empty vector respectively. <br/>
Line 45: Line 45:
<br/>
<br/>
These results show that <b>the percentage of adhesion is similar between the strains containing the three parts and the positive control, thus tagged CsgA were still functional</b>. CsgA with one or two tags from the P70 promoter were sufficient to form thick biofilms. </p><br/>
These results show that <b>the percentage of adhesion is similar between the strains containing the three parts and the positive control, thus tagged CsgA were still functional</b>. CsgA with one or two tags from the P70 promoter were sufficient to form thick biofilms. </p><br/>
-
<div align=”center”><img src=https://static.igem.org/mediawiki/2014/0/0c/Crystal_violet006.png align=”center” alt="Figure 2 : Engineered bacteria Biofilm formation"/></div>
+
<div align=”center”><img src=https://static.igem.org/mediawiki/2014/d/dc/Crystal_violet_2.png align=”center” alt="Figure 2 : Engineered bacteria Biofilm formation"/></div>
<b>Figure 2 : Engineered bacteria Biofilm formation</b><br/>
<b>Figure 2 : Engineered bacteria Biofilm formation</b><br/>
  <p align=" justify ">The cells were grown as described as figure 1. <br/>
  <p align=" justify ">The cells were grown as described as figure 1. <br/>

Revision as of 22:52, 17 October 2014

Curly'on - IGEM 2014 INSA-LYON

  • Curli characterization


  • Nickel chelation


  • Survival after UV and high temperature exposure


  • Promoter optimization and characterization