Team:INSA-Lyon/Results

From 2014.igem.org

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<p>Firstly, a <b> calibration curve </b> of the formation Nickel and DMG complexes was established. </p>
<p>Firstly, a <b> calibration curve </b> of the formation Nickel and DMG complexes was established. </p>
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<p>Then, strains were assayed for biofilm nickel absorption on liquid cultures using the calibration curve, measuring the OD of the complex formed for each strain at 554nm. (<a href="https://static.igem.org/mediawiki/2014/0/01/Ni_chelation_DMG_n.pdf"> See Protocol for details</a>)<br/>
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<p>Then, strains were assayed for biofilm nickel absorption on liquid cultures using the calibration curve, measuring the OD of the complex formed for each strain at 554nm. (<a href="https://static.igem.org/mediawiki/2014/0/01/Ni_chelation_DMG_n.pdf">See Protocol for details</a>)<br/>
Although quantification is possible, this technique lacks precision and is more suited for <b>qualitative</b> studies. However, it is a cheaper alternative to ICP-MS. </p>
Although quantification is possible, this technique lacks precision and is more suited for <b>qualitative</b> studies. However, it is a cheaper alternative to ICP-MS. </p>

Revision as of 22:25, 17 October 2014

Curly'on - IGEM 2014 INSA-LYON

  • Curli characterization


  • Nickel chelation


  • Survival after UV and high temperature exposure


  • Promoter optimization and characterization