Team:INSA-Lyon/Parts

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<h4>PARTS</h4>
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<a href="https://2014.igem.org/Team:INSA-Lyon/Biology" class="hu-icon"><li class="iconmulti">WETLAB SUMMARY</li></a>
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<a href="https://2014.igem.org/Team:INSA-Lyon/Results" class="hu-icon"><li class="icon">RESULTS</li></a>
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<a href="https://2014.igem.org/Team:INSA-Lyon/Notebook" class="hu-icon"><li class="icon">NOTEBOOK</li></a>
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<a href="https://2014.igem.org/Team:INSA-Lyon/Protocol" class="hu-icon"><li class="icon">PROTOCOLS</li></a>
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<a href="https://2014.igem.org/Team:INSA-Lyon/DataPage" class="hu-icon"><li class="icon">DATA PAGE</li></a>
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<h1 >WELCOME TO iGEM 2014! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season!
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:INSA-Lyon/Parts&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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<p>5 biobricks have been constructed, thre for Nickel chelation, two for promoter characterization.</p></br>
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<b>Biobricks for nickel chelation</b></br></br>
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<p>The principal of the construction is shown in the figure below.</p></br></br></br></div>
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<img src= "https://static.igem.org/mediawiki/parts/6/63/1.JPG"></br>
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<a href="https://2014.igem.org/Team:INSA-Lyon"style="color:#000000">Home </a> </td>
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<p>On a pSB1C3 backbone, two essential part have been assembled: a promoter and a reporter coding for CsgA.The promoter in this case is always the same: P70.However, the reporter is different for each construction.</br>
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The first CsgA is called CsgA WT, the sequence is exactly the same as we found in a wild type CsgA protein except one mutation in order to eliminate one restriction site. The construction is also called CsgA-WT.</br>
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The second CsgA is based on the same sequence but with a short sequence coding for a his tag(a peptide chain coding six histidines). This construction is called CsgA-His1.</br>
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The last CsgA is almost the same but with 2 his tag. This construction is called CsgA-His2.</br>
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<a href="https://2014.igem.org/Team:INSA-Lyon/Team"style="color:#000000"> Team </a> </td>
 
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<a href="https://igem.org/Team.cgi?year=2014&team_name=INSA-Lyon"style="color:#000000"> Official Team Profile </a></td>
 
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<a href="https://2014.igem.org/Team:INSA-Lyon/Project"style="color:#000000"> Project</a></td>
 
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<a href="https://2014.igem.org/Team:INSA-Lyon/Parts"style="color:#000000"> Parts</a></td>
 
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<a href="https://2014.igem.org/Team:INSA-Lyon/Modeling"style="color:#000000"> Modeling</a></td>
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<a href="https://2014.igem.org/Team:INSA-Lyon/Notebook"style="color:#000000"> Notebook</a></td>
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<a href="https://2014.igem.org/Team:INSA-Lyon/Safety"style=" color:#000000"> Safety </a></td>
 
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<a href="https://2014.igem.org/Team:INSA-Lyon/Attributions"style="color:#000000"> Attributions </a></td>
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<center><groupparts>iGEM014 INSA-Lyon</groupparts></center>
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<td align ="center"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"></a> </td>
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<tr><td > <h3> Parts Submitted to the Registry </h3></td>
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<td > <h3>What information do I need to start putting my parts on the Registry? </h3></td>
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An important aspect of the iGEM competition is the use and creation of standard  biological parts. Each team will make new parts during iGEM and will submit them to the <a href="http://partsregistry.org"> Registry of Standard Biological Parts</a>. The iGEM software provides an easy way to present the parts your team has created. The "groupparts" tag will generate a table with all of the parts that your team adds to your team sandbox. 
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<strong>Note that if you want to document a part you need to document it on the <a href="http://partsregistry.org Registry"> Registry</a>, not on your team wiki.</strong> Future teams and other users and are much more likely to find parts on the Registry than on your team wiki.
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Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without a need to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.
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<h3>When should you put parts into the Registry?</h3>
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As soon as possible! We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better recall you will have of all details surrounding your parts. Remember you don't need to send us the DNA to create an entry for a part on the Registry. However, you must send us the sample/DNA before the Jamboree. Only parts for which you have sent us samples/DNA are eligible for awards and medal requirements.
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The information needed to initially create a part on the Registry is:
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<li>Part Name</li>
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<li>Short Description (60 characters on what the DNA does)</li>
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<li>Long Description (Longer description of what the DNA does)</li>
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<li>Design considerations</li>
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. Check out part <a href="http://parts.igem.org/Part:BBa_K404003">BBa_K404003</a> for an excellent example of a highly characterized part.
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You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry"> Add a Part to the Registry</a> link.
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<tr><td colspan="3" > <h3> Parts Table</h3></td></tr>
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Any parts your team has created will appear in this table below:</td></tr>
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<groupparts>iGEM013 INSA-Lyon</groupparts>
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Latest revision as of 17:07, 17 October 2014

Curly'on - IGEM 2014 INSA-LYON

  • 5 biobricks have been constructed, thre for Nickel chelation, two for promoter characterization.


    Biobricks for nickel chelation

    The principal of the construction is shown in the figure below.





  • On a pSB1C3 backbone, two essential part have been assembled: a promoter and a reporter coding for CsgA.The promoter in this case is always the same: P70.However, the reporter is different for each construction.
    The first CsgA is called CsgA WT, the sequence is exactly the same as we found in a wild type CsgA protein except one mutation in order to eliminate one restriction site. The construction is also called CsgA-WT.
    The second CsgA is based on the same sequence but with a short sequence coding for a his tag(a peptide chain coding six histidines). This construction is called CsgA-His1.
    The last CsgA is almost the same but with 2 his tag. This construction is called CsgA-His2.

    • <groupparts>iGEM014 INSA-Lyon</groupparts>

    Retrieved from "http://2014.igem.org/Team:INSA-Lyon/Parts"