Team:INSA-Lyon/Notebook

From 2014.igem.org

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           <ul id="contenu6" style="list-style-type: none !important;display:none;">
           <ul id="contenu6" style="list-style-type: none !important;display:none;">
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<h6>2/10 </h6>
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Pre-cultures of the negative control, positive control, PHC46 (P70-GFP) and PHC47(P750-GFP).
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<h6>3/10 </h6>
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We distributed the blank, growth control, positive control for GFP expression, PHC46 (P70-GFP) and PHC47 (P750-GFP) in a 96 well plate and explored the GFP expression at 37 degrees.
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</p>
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<p>
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We distributed the blank, growth control, positive control for GFP expression, PHC46 (P70-GFP) and PHC47 (P750-GFP) in a 96 well plate and explored the GFP expression at 37°C.
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</p>
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<h6>3/10 </h6>
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We gathered the results at 37°C and interpreted the results of GFP expression from the P70 or P750 long promoter upstream.
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<h6>4/10 </h6>
<h6>4/10 </h6>
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<p> Petri dish nickel test on the Petri dishes containing the best clones for each one of the newly transformed strains 224, 266, 267.</p>
<p> Petri dish nickel test on the Petri dishes containing the best clones for each one of the newly transformed strains 224, 266, 267.</p>
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<p>
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Pre-cultures of the negative control, positive control, PHC46 (P70-GFP) and PHC47(P750-GFP).
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</p>
<h6>8/10  </h6>
<h6>8/10  </h6>
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<p>Cultured the best clones for each one of the newly transformed strains 224, 266, 267, 325 and 334 in a 24 wells plate. </p>
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<p>
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Cultured the best clones for each one of the newly transformed strains 224, 266, 267, 325 and 334 in a 24 wells plate.
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</p>
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<p>
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We distributed the blank, growth control, positive control for GFP expression, PHC46 (P70-GFP) and PHC47 (P750-GFP) in a 96 well plate and explored the GFP expression at 30 degrees.
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</p>
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<p> Red congo and adherence tests on the newly transformed strains 224, 266, 267, 325 and 334 cultured in the 24 wells plate.</p>
<p> Red congo and adherence tests on the newly transformed strains 224, 266, 267, 325 and 334 cultured in the 24 wells plate.</p>
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<p>
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We interpreted and compared the results of GFP expression from the P70 or P750 long promoter upstream.
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</p>
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<br/>
<br/>

Revision as of 18:14, 17 October 2014

Curly'on - IGEM 2014 INSA-LYON

Here you can find a summary of all the experiments we made throughout the project.

To download a pdf with plasmids' description, resistance and names click here

To download a pdf with the strains' description, resistance and names click here


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