http://2014.igem.org/wiki/index.php?title=Team:INSA-Lyon/Biology&feed=atom&action=historyTeam:INSA-Lyon/Biology - Revision history2024-03-29T13:40:12ZRevision history for this page on the wikiMediaWiki 1.16.5http://2014.igem.org/wiki/index.php?title=Team:INSA-Lyon/Biology&diff=393894&oldid=prevSuxiaohui at 03:05, 18 October 20142014-10-18T03:05:36Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li> <p>We designed a protocol for <a href="https://static.igem.org/mediawiki/2014/0/01/Ni_chelation_DMG_n.pdf">nickel quantification using dimethylglyoxime (DMG)</a> that changes color from transparent to bright red in the presence of nickel and confirmed those results using mass spectrometry.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li> <p>We designed a protocol for <a href="https://static.igem.org/mediawiki/2014/0/01/Ni_chelation_DMG_n.pdf">nickel quantification using dimethylglyoxime (DMG)</a> that changes color from transparent to bright red in the presence of nickel and confirmed those results using mass spectrometry.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li><p>We explored the biofilm production of our strain by testing its <a href="https://static.igem.org/mediawiki/2014/8/80/Adhesion_test_protocole.pdf">adhesion ability</a> and its curli synthesis with the <a href="https://static.igem.org/mediawiki/2014/3/39/CongoRed.pdf">Congo Red dye</a> and further visualized it by Transmission Electronic Microscopy (TEM) and <a href="https://static.igem.org/mediawiki/2014/7/7e/Culture_confocal_analyse.pdf">Confocal microscopy</a>.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li><p>We explored the biofilm production of our strain by testing its <a href="https://static.igem.org/mediawiki/2014/8/80/Adhesion_test_protocole.pdf">adhesion ability</a> and its curli synthesis with the <a href="https://static.igem.org/mediawiki/2014/3/39/CongoRed.pdf">Congo Red dye</a> and further visualized it by Transmission Electronic Microscopy (TEM) and <a href="https://static.igem.org/mediawiki/2014/7/7e/Culture_confocal_analyse.pdf">Confocal microscopy</a>.</p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><li><p>We investigated the bacterial survival after increased exposure to <a href="https://static.igem.org/mediawiki/2014/1/1e/UV_temperature.pdf"> UV light and high temperatures</a>. The purpose is to optimize <del class="diffchange diffchange-inline">sterilisation </del>methods for future filter design.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><li><p>We investigated the bacterial survival after increased exposure to <a href="https://static.igem.org/mediawiki/2014/1/1e/UV_temperature.pdf"> UV light and high temperatures</a>. The purpose is to optimize <ins class="diffchange diffchange-inline">sterilization </ins>methods for future filter design.</p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><li><p>We engineered and simplified the promoter sequences responsible for CsgA production. In fact,in the WT strain, <del class="diffchange diffchange-inline">CsgA </del>expression is controlled by the csgABC promoter but we identified, isolated and characterized a 70 base-pairs sequence that reaches higher production rates at 37°C instead of 30°C.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><li><p>We engineered and simplified the promoter sequences responsible for CsgA production. In fact,in the WT strain, <ins class="diffchange diffchange-inline"><i>csgA</i> </ins>expression is controlled by the <ins class="diffchange diffchange-inline"><i></ins>csgABC<ins class="diffchange diffchange-inline"></i> </ins>promoter but we identified, isolated and characterized a 70 base-pairs sequence that reaches higher production rates at 37°C instead of 30°C.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></ul></div></td></tr>
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</table>Suxiaohuihttp://2014.igem.org/wiki/index.php?title=Team:INSA-Lyon/Biology&diff=380753&oldid=prevJulietteP at 01:14, 18 October 20142014-10-18T01:14:37Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li> <p>We constructed and cloned a modified CsgA that has either one or two His-Tag motifs. This way, we will be able to investigate if a repeated His-Tag motif is able to chelate more nickel or not.</p> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li> <p>We constructed and cloned a modified CsgA that has either one or two His-Tag motifs. This way, we will be able to investigate if a repeated His-Tag motif is able to chelate more nickel or not.</p> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li> <p>We designed a protocol for <a href="https://static.igem.org/mediawiki/2014/0/01/Ni_chelation_DMG_n.pdf">nickel quantification using dimethylglyoxime (DMG)</a> that changes color from transparent to bright red in the presence of nickel and confirmed those results using mass spectrometry.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li> <p>We designed a protocol for <a href="https://static.igem.org/mediawiki/2014/0/01/Ni_chelation_DMG_n.pdf">nickel quantification using dimethylglyoxime (DMG)</a> that changes color from transparent to bright red in the presence of nickel and confirmed those results using mass spectrometry.</p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><li><p>We explored the biofilm production of our strain by testing its <a href="https://static.igem.org/mediawiki/2014/8/80/Adhesion_test_protocole.pdf">adhesion ability</a> and its curli synthesis with the<a href="https://static.igem.org/mediawiki/2014/3/39/CongoRed.pdf">Congo Red dye</a> and further visualized it by Transmission Electronic Microscopy (TEM) and <a href="https://static.igem.org/mediawiki/2014/7/7e/Culture_confocal_analyse.pdf">Confocal microscopy</a>.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><li><p>We explored the biofilm production of our strain by testing its <a href="https://static.igem.org/mediawiki/2014/8/80/Adhesion_test_protocole.pdf">adhesion ability</a> and its curli synthesis with the <a href="https://static.igem.org/mediawiki/2014/3/39/CongoRed.pdf">Congo Red dye</a> and further visualized it by Transmission Electronic Microscopy (TEM) and <a href="https://static.igem.org/mediawiki/2014/7/7e/Culture_confocal_analyse.pdf">Confocal microscopy</a>.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li><p>We investigated the bacterial survival after increased exposure to <a href="https://static.igem.org/mediawiki/2014/1/1e/UV_temperature.pdf"> UV light and high temperatures</a>. The purpose is to optimize sterilisation methods for future filter design.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li><p>We investigated the bacterial survival after increased exposure to <a href="https://static.igem.org/mediawiki/2014/1/1e/UV_temperature.pdf"> UV light and high temperatures</a>. The purpose is to optimize sterilisation methods for future filter design.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li><p>We engineered and simplified the promoter sequences responsible for CsgA production. In fact,in the WT strain, CsgA expression is controlled by the csgABC promoter but we identified, isolated and characterized a 70 base-pairs sequence that reaches higher production rates at 37°C instead of 30°C.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li><p>We engineered and simplified the promoter sequences responsible for CsgA production. In fact,in the WT strain, CsgA expression is controlled by the csgABC promoter but we identified, isolated and characterized a 70 base-pairs sequence that reaches higher production rates at 37°C instead of 30°C.</p></div></td></tr>
</table>JuliettePhttp://2014.igem.org/wiki/index.php?title=Team:INSA-Lyon/Biology&diff=380593&oldid=prevJulietteP at 01:13, 18 October 20142014-10-18T01:13:13Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li> <p>We constructed and cloned a modified CsgA that has either one or two His-Tag motifs. This way, we will be able to investigate if a repeated His-Tag motif is able to chelate more nickel or not.</p> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li> <p>We constructed and cloned a modified CsgA that has either one or two His-Tag motifs. This way, we will be able to investigate if a repeated His-Tag motif is able to chelate more nickel or not.</p> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li> <p>We designed a protocol for <a href="https://static.igem.org/mediawiki/2014/0/01/Ni_chelation_DMG_n.pdf">nickel quantification using dimethylglyoxime (DMG)</a> that changes color from transparent to bright red in the presence of nickel and confirmed those results using mass spectrometry.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li> <p>We designed a protocol for <a href="https://static.igem.org/mediawiki/2014/0/01/Ni_chelation_DMG_n.pdf">nickel quantification using dimethylglyoxime (DMG)</a> that changes color from transparent to bright red in the presence of nickel and confirmed those results using mass spectrometry.</p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><li><p>We explored the biofilm production of our strain by testing its adhesion ability and its curli synthesis with <a href="https://static.igem.org/mediawiki/2014/3/39/CongoRed.pdf"><del class="diffchange diffchange-inline">the </del>Congo Red dye</a> and further visualized it by Transmission Electronic Microscopy (TEM) and Confocal microscopy.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><li><p>We explored the biofilm production of our strain by testing its <ins class="diffchange diffchange-inline"><a href="https://static.igem.org/mediawiki/2014/8/80/Adhesion_test_protocole.pdf"></ins>adhesion ability<ins class="diffchange diffchange-inline"></a> </ins>and its curli synthesis with <ins class="diffchange diffchange-inline">the</ins><a href="https://static.igem.org/mediawiki/2014/3/39/CongoRed.pdf">Congo Red dye</a> and further visualized it by Transmission Electronic Microscopy (TEM) and <ins class="diffchange diffchange-inline"><a href="https://static.igem.org/mediawiki/2014/7/7e/Culture_confocal_analyse.pdf"></ins>Confocal microscopy<ins class="diffchange diffchange-inline"></a></ins>.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li><p>We investigated the bacterial survival after increased exposure to <a href="https://static.igem.org/mediawiki/2014/1/1e/UV_temperature.pdf"> UV light and high temperatures</a>. The purpose is to optimize sterilisation methods for future filter design.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li><p>We investigated the bacterial survival after increased exposure to <a href="https://static.igem.org/mediawiki/2014/1/1e/UV_temperature.pdf"> UV light and high temperatures</a>. The purpose is to optimize sterilisation methods for future filter design.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li><p>We engineered and simplified the promoter sequences responsible for CsgA production. In fact,in the WT strain, CsgA expression is controlled by the csgABC promoter but we identified, isolated and characterized a 70 base-pairs sequence that reaches higher production rates at 37°C instead of 30°C.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li><p>We engineered and simplified the promoter sequences responsible for CsgA production. In fact,in the WT strain, CsgA expression is controlled by the csgABC promoter but we identified, isolated and characterized a 70 base-pairs sequence that reaches higher production rates at 37°C instead of 30°C.</p></div></td></tr>
</table>JuliettePhttp://2014.igem.org/wiki/index.php?title=Team:INSA-Lyon/Biology&diff=379497&oldid=prevJulietteP at 01:04, 18 October 20142014-10-18T01:04:02Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li> <p>We constructed and cloned a modified CsgA that has either one or two His-Tag motifs. This way, we will be able to investigate if a repeated His-Tag motif is able to chelate more nickel or not.</p> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li> <p>We constructed and cloned a modified CsgA that has either one or two His-Tag motifs. This way, we will be able to investigate if a repeated His-Tag motif is able to chelate more nickel or not.</p> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li> <p>We designed a protocol for <a href="https://static.igem.org/mediawiki/2014/0/01/Ni_chelation_DMG_n.pdf">nickel quantification using dimethylglyoxime (DMG)</a> that changes color from transparent to bright red in the presence of nickel and confirmed those results using mass spectrometry.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li> <p>We designed a protocol for <a href="https://static.igem.org/mediawiki/2014/0/01/Ni_chelation_DMG_n.pdf">nickel quantification using dimethylglyoxime (DMG)</a> that changes color from transparent to bright red in the presence of nickel and confirmed those results using mass spectrometry.</p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><li><p>We explored the biofilm production of our strain <del class="diffchange diffchange-inline">and </del>its <del class="diffchange diffchange-inline">adherence </del>ability <del class="diffchange diffchange-inline">by using </del><a href="https://static.igem.org/mediawiki/2014/3/39/CongoRed.pdf">the Congo Red dye</a> and further visualized it by Transmission Electronic Microscopy (TEM).</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><li><p>We explored the biofilm production of our strain <ins class="diffchange diffchange-inline">by testing </ins>its <ins class="diffchange diffchange-inline">adhesion </ins>ability <ins class="diffchange diffchange-inline">and its curli synthesis with </ins><a href="https://static.igem.org/mediawiki/2014/3/39/CongoRed.pdf">the Congo Red dye</a> and further visualized it by Transmission Electronic Microscopy (TEM) <ins class="diffchange diffchange-inline">and Confocal microscopy</ins>.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li><p>We investigated the bacterial survival after increased exposure to <a href="https://static.igem.org/mediawiki/2014/1/1e/UV_temperature.pdf"> UV light and high temperatures</a>. The purpose is to optimize sterilisation methods for future filter design.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li><p>We investigated the bacterial survival after increased exposure to <a href="https://static.igem.org/mediawiki/2014/1/1e/UV_temperature.pdf"> UV light and high temperatures</a>. The purpose is to optimize sterilisation methods for future filter design.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li><p>We engineered and simplified the promoter sequences responsible for CsgA production. In fact,in the WT strain, CsgA expression is controlled by the csgABC promoter but we identified, isolated and characterized a 70 base-pairs sequence that reaches higher production rates at 37°C instead of 30°C.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li><p>We engineered and simplified the promoter sequences responsible for CsgA production. In fact,in the WT strain, CsgA expression is controlled by the csgABC promoter but we identified, isolated and characterized a 70 base-pairs sequence that reaches higher production rates at 37°C instead of 30°C.</p></div></td></tr>
</table>JuliettePhttp://2014.igem.org/wiki/index.php?title=Team:INSA-Lyon/Biology&diff=377957&oldid=prevJulietteP at 00:51, 18 October 20142014-10-18T00:51:18Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><b>Our wetlab work focuses on designing a bacterial strain able to chelate as much <del class="diffchange diffchange-inline">Nickel </del>as possible and adhere to a synthetic matrix for future filter design applications</b>. To do so, we engineered a hair-shaped protein polymer located at the bacterial surface, called curli (<a href="http://www.ncbi.nlm.nih.gov/pubmed/16704339">Barnhart 2006</a>). CsgA, which is the monomer of the <del class="diffchange diffchange-inline">Curli </del>structure, can be engineered. This property constitutes the basis of our work, as we modified the CsgA by adding one or more His-Tag motifs, famously known to be able to chelate <del class="diffchange diffchange-inline">Nickel </del>(<a href="http://www.nature.com/nbt/journal/v6/n11/full/nbt1188-1321.html">Hochuli 1988</a>). Our project aims at engineering an <i>Escherichia coli</i> strain that naturally produces abundant biofilm to make her produce the engineered curli proteins at the same time. This way, the bacteria have the sufficient adhesion ability to stick to the filter matrix and be exposed to polluted water while chelating the environmental nickel.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><b>Our wetlab work focuses on designing a bacterial strain able to chelate as much <ins class="diffchange diffchange-inline">nickel </ins>as possible and adhere to a synthetic matrix for future filter design applications</b>. To do so, we engineered a hair-shaped protein polymer located at the bacterial surface, called curli (<a href="http://www.ncbi.nlm.nih.gov/pubmed/16704339">Barnhart 2006</a>). CsgA, which is the monomer of the <ins class="diffchange diffchange-inline">curli </ins>structure, can be engineered. This property constitutes the basis of our work, as we modified the CsgA by adding one or more His-Tag motifs, famously known to be able to chelate <ins class="diffchange diffchange-inline">nickel </ins>(<a href="http://www.nature.com/nbt/journal/v6/n11/full/nbt1188-1321.html">Hochuli 1988</a>). Our project aims at engineering an <i>Escherichia coli</i> strain that naturally produces abundant biofilm to make her produce the engineered curli proteins at the same time. This way, the bacteria have the sufficient adhesion ability to stick to the filter matrix and be exposed to polluted water while chelating the environmental nickel.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><ul></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><li> <p>We constructed and cloned a modified CsgA that has either one or two His-Tag motifs. This way, we will be able to investigate if a repeated His-Tag motif is able to chelate more <del class="diffchange diffchange-inline">Nickel </del>or not.</p> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><li> <p>We constructed and cloned a modified CsgA that has either one or two His-Tag motifs. This way, we will be able to investigate if a repeated His-Tag motif is able to chelate more <ins class="diffchange diffchange-inline">nickel </ins>or not.</p> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><li> <p>We designed a protocol for <a href="https://static.igem.org/mediawiki/2014/0/01/Ni_chelation_DMG_n.pdf">nickel quantification using dimethylglyoxime (DMG)</a> that changes color from transparent to bright red in the presence of <del class="diffchange diffchange-inline">Nickel </del>and confirmed those results using mass spectrometry.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><li> <p>We designed a protocol for <a href="https://static.igem.org/mediawiki/2014/0/01/Ni_chelation_DMG_n.pdf">nickel quantification using dimethylglyoxime (DMG)</a> that changes color from transparent to bright red in the presence of <ins class="diffchange diffchange-inline">nickel </ins>and confirmed those results using mass spectrometry.</p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><li><p>We explored the biofilm production of our strain and its adherence ability by using the Congo Red dye and further visualized it by Transmission Electronic Microscopy (TEM).</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><li><p>We explored the biofilm production of our strain and its adherence ability by using <ins class="diffchange diffchange-inline"><a href="https://static.igem.org/mediawiki/2014/3/39/CongoRed.pdf"></ins>the Congo Red dye<ins class="diffchange diffchange-inline"></a> </ins>and further visualized it by Transmission Electronic Microscopy (TEM).</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li><p>We investigated the bacterial survival after increased exposure to <a href="https://static.igem.org/mediawiki/2014/1/1e/UV_temperature.pdf"> UV light and high temperatures</a>. The purpose is to optimize sterilisation methods for future filter design.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li><p>We investigated the bacterial survival after increased exposure to <a href="https://static.igem.org/mediawiki/2014/1/1e/UV_temperature.pdf"> UV light and high temperatures</a>. The purpose is to optimize sterilisation methods for future filter design.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li><p>We engineered and simplified the promoter sequences responsible for CsgA production. In fact,in the WT strain, CsgA expression is controlled by the csgABC promoter but we identified, isolated and characterized a 70 base-pairs sequence that reaches higher production rates at 37°C instead of 30°C.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li><p>We engineered and simplified the promoter sequences responsible for CsgA production. In fact,in the WT strain, CsgA expression is controlled by the csgABC promoter but we identified, isolated and characterized a 70 base-pairs sequence that reaches higher production rates at 37°C instead of 30°C.</p></div></td></tr>
</table>JuliettePhttp://2014.igem.org/wiki/index.php?title=Team:INSA-Lyon/Biology&diff=372600&oldid=prevChoupx at 00:05, 18 October 20142014-10-18T00:05:31Z<p></p>
<table style="background-color: white; color:black;">
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<td colspan='2' style="background-color: white; color:black;">Revision as of 00:05, 18 October 2014</td>
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<td colspan="2" class="diff-lineno">Line 36:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li> <p>We designed a protocol for <a href="https://static.igem.org/mediawiki/2014/0/01/Ni_chelation_DMG_n.pdf">nickel quantification using dimethylglyoxime (DMG)</a> that changes color from transparent to bright red in the presence of Nickel and confirmed those results using mass spectrometry.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li> <p>We designed a protocol for <a href="https://static.igem.org/mediawiki/2014/0/01/Ni_chelation_DMG_n.pdf">nickel quantification using dimethylglyoxime (DMG)</a> that changes color from transparent to bright red in the presence of Nickel and confirmed those results using mass spectrometry.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li><p>We explored the biofilm production of our strain and its adherence ability by using the Congo Red dye and further visualized it by Transmission Electronic Microscopy (TEM).</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li><p>We explored the biofilm production of our strain and its adherence ability by using the Congo Red dye and further visualized it by Transmission Electronic Microscopy (TEM).</p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><li><p>We investigated the bacterial survival after increased exposure to <a href="https://static.igem.org/mediawiki/2014/1/1e/UV_temperature.pdf"> UV light and high temperatures </a>. The purpose is to optimize sterilisation methods for future filter design.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><li><p>We investigated the bacterial survival after increased exposure to <a href="https://static.igem.org/mediawiki/2014/1/1e/UV_temperature.pdf"> UV light and high temperatures</a>. The purpose is to optimize sterilisation methods for future filter design.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li><p>We engineered and simplified the promoter sequences responsible for CsgA production. In fact,in the WT strain, CsgA expression is controlled by the csgABC promoter but we identified, isolated and characterized a 70 base-pairs sequence that reaches higher production rates at 37°C instead of 30°C.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li><p>We engineered and simplified the promoter sequences responsible for CsgA production. In fact,in the WT strain, CsgA expression is controlled by the csgABC promoter but we identified, isolated and characterized a 70 base-pairs sequence that reaches higher production rates at 37°C instead of 30°C.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></ul></div></td></tr>
</table>Choupxhttp://2014.igem.org/wiki/index.php?title=Team:INSA-Lyon/Biology&diff=372535&oldid=prevChoupx at 00:05, 18 October 20142014-10-18T00:05:02Z<p></p>
<table style="background-color: white; color:black;">
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<td colspan="2" class="diff-lineno">Line 36:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li> <p>We designed a protocol for <a href="https://static.igem.org/mediawiki/2014/0/01/Ni_chelation_DMG_n.pdf">nickel quantification using dimethylglyoxime (DMG)</a> that changes color from transparent to bright red in the presence of Nickel and confirmed those results using mass spectrometry.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li> <p>We designed a protocol for <a href="https://static.igem.org/mediawiki/2014/0/01/Ni_chelation_DMG_n.pdf">nickel quantification using dimethylglyoxime (DMG)</a> that changes color from transparent to bright red in the presence of Nickel and confirmed those results using mass spectrometry.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li><p>We explored the biofilm production of our strain and its adherence ability by using the Congo Red dye and further visualized it by Transmission Electronic Microscopy (TEM).</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li><p>We explored the biofilm production of our strain and its adherence ability by using the Congo Red dye and further visualized it by Transmission Electronic Microscopy (TEM).</p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><li><p>We investigated the bacterial survival after increased exposure to UV light and high temperatures. The purpose is to optimize sterilisation methods for future filter design.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><li><p>We investigated the bacterial survival after increased exposure to <ins class="diffchange diffchange-inline"><a href="https://static.igem.org/mediawiki/2014/1/1e/UV_temperature.pdf"> </ins>UV light and high temperatures <ins class="diffchange diffchange-inline"></a></ins>. The purpose is to optimize sterilisation methods for future filter design.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li><p>We engineered and simplified the promoter sequences responsible for CsgA production. In fact,in the WT strain, CsgA expression is controlled by the csgABC promoter but we identified, isolated and characterized a 70 base-pairs sequence that reaches higher production rates at 37°C instead of 30°C.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li><p>We engineered and simplified the promoter sequences responsible for CsgA production. In fact,in the WT strain, CsgA expression is controlled by the csgABC promoter but we identified, isolated and characterized a 70 base-pairs sequence that reaches higher production rates at 37°C instead of 30°C.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></ul></div></td></tr>
</table>Choupxhttp://2014.igem.org/wiki/index.php?title=Team:INSA-Lyon/Biology&diff=371037&oldid=prevChoupx at 23:52, 17 October 20142014-10-17T23:52:50Z<p></p>
<table style="background-color: white; color:black;">
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<td colspan='2' style="background-color: white; color:black;">Revision as of 23:52, 17 October 2014</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><ul></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li> <p>We constructed and cloned a modified CsgA that has either one or two His-Tag motifs. This way, we will be able to investigate if a repeated His-Tag motif is able to chelate more Nickel or not.</p> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li> <p>We constructed and cloned a modified CsgA that has either one or two His-Tag motifs. This way, we will be able to investigate if a repeated His-Tag motif is able to chelate more Nickel or not.</p> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><li> <p>We designed a protocol for nickel quantification using dimethylglyoxime (DMG) that changes color from transparent to bright red in the presence of Nickel and confirmed those results using mass spectrometry.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><li> <p>We designed a protocol for <ins class="diffchange diffchange-inline"><a href="https://static.igem.org/mediawiki/2014/0/01/Ni_chelation_DMG_n.pdf"></ins>nickel quantification using dimethylglyoxime (DMG)<ins class="diffchange diffchange-inline"></a> </ins>that changes color from transparent to bright red in the presence of Nickel and confirmed those results using mass spectrometry.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li><p>We explored the biofilm production of our strain and its adherence ability by using the Congo Red dye and further visualized it by Transmission Electronic Microscopy (TEM).</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li><p>We explored the biofilm production of our strain and its adherence ability by using the Congo Red dye and further visualized it by Transmission Electronic Microscopy (TEM).</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li><p>We investigated the bacterial survival after increased exposure to UV light and high temperatures. The purpose is to optimize sterilisation methods for future filter design.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li><p>We investigated the bacterial survival after increased exposure to UV light and high temperatures. The purpose is to optimize sterilisation methods for future filter design.</p></div></td></tr>
</table>Choupxhttp://2014.igem.org/wiki/index.php?title=Team:INSA-Lyon/Biology&diff=360266&oldid=prevBenoitdrogue at 22:11, 17 October 20142014-10-17T22:11:08Z<p></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 22:11, 17 October 2014</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div align="justify"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div align="justify"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><b>Our wetlab work focuses on designing a bacterial strain able to chelate as much Nickel as possible and adhere to a synthetic matrix for future filter design applications</b>. To do so, we engineered a hair-shaped protein polymer located at the bacterial surface, called curli (<a href="http://www.ncbi.nlm.nih.gov/pubmed/16704339">Barnhart 2006</a>). CsgA, which is the monomer of the Curli structure, can be engineered. This property constitutes the basis of our work, as we modified the CsgA by adding one or more His-Tag motifs, famously known to be able to chelate Nickel (<a href="http://www.nature.com/nbt/journal/v6/n11/full/nbt1188-1321.html">Hochuli 1988</a>). Our project aims at <del class="diffchange diffchange-inline">modifying </del>an <i>Escherichia coli</i> strain that naturally produces abundant biofilm <del class="diffchange diffchange-inline">and produces </del>the engineered curli proteins at the same time. This way, the bacteria have the sufficient adhesion ability to stick to the filter matrix and be exposed to polluted water while chelating the environmental nickel.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><b>Our wetlab work focuses on designing a bacterial strain able to chelate as much Nickel as possible and adhere to a synthetic matrix for future filter design applications</b>. To do so, we engineered a hair-shaped protein polymer located at the bacterial surface, called curli (<a href="http://www.ncbi.nlm.nih.gov/pubmed/16704339">Barnhart 2006</a>). CsgA, which is the monomer of the Curli structure, can be engineered. This property constitutes the basis of our work, as we modified the CsgA by adding one or more His-Tag motifs, famously known to be able to chelate Nickel (<a href="http://www.nature.com/nbt/journal/v6/n11/full/nbt1188-1321.html">Hochuli 1988</a>). Our project aims at <ins class="diffchange diffchange-inline">engineering </ins>an <i>Escherichia coli</i> strain that naturally produces abundant biofilm <ins class="diffchange diffchange-inline">to make her produce </ins>the engineered curli proteins at the same time. This way, the bacteria have the sufficient adhesion ability to stick to the filter matrix and be exposed to polluted water while chelating the environmental nickel.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td></tr>
</table>Benoitdroguehttp://2014.igem.org/wiki/index.php?title=Team:INSA-Lyon/Biology&diff=359421&oldid=prevBenoitdrogue at 22:02, 17 October 20142014-10-17T22:02:39Z<p></p>
<table style="background-color: white; color:black;">
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<td colspan='2' style="background-color: white; color:black;">Revision as of 22:02, 17 October 2014</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 25:</td>
<td colspan="2" class="diff-lineno">Line 25:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div align="justify"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div align="justify"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Our wetlab work focuses on designing a bacterial strain able to chelate as much Nickel as possible <del class="diffchange diffchange-inline">but also to </del>adhere to a synthetic matrix for future filter design applications. To do so, we engineered a hair-shaped protein polymer located at the bacterial surface, called curli (<a href="http://www.ncbi.nlm.nih.gov/pubmed/16704339">Barnhart 2006</a>). CsgA, which is the monomer of the Curli structure, can be engineered. This property constitutes the basis of our work, as we <del class="diffchange diffchange-inline">engineered </del>the CsgA by adding one or more His-Tag motifs, famously known to be able to chelate Nickel (<a href="http://www.nature.com/nbt/journal/v6/n11/full/nbt1188-1321.html">Hochuli 1988</a>). Our project aims at modifying an <i>Escherichia coli</i> strain that naturally produces abundant biofilm and <del class="diffchange diffchange-inline">at the same time </del>produces the engineered curli proteins <del class="diffchange diffchange-inline">to chelate Nickel</del>. This way, the bacteria have the sufficient adhesion ability to stick to the filter matrix and be exposed to polluted water while chelating the environmental nickel.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><b></ins>Our wetlab work focuses on designing a bacterial strain able to chelate as much Nickel as possible <ins class="diffchange diffchange-inline">and </ins>adhere to a synthetic matrix for future filter design applications<ins class="diffchange diffchange-inline"></b></ins>. To do so, we engineered a hair-shaped protein polymer located at the bacterial surface, called curli (<a href="http://www.ncbi.nlm.nih.gov/pubmed/16704339">Barnhart 2006</a>). CsgA, which is the monomer of the Curli structure, can be engineered. This property constitutes the basis of our work, as we <ins class="diffchange diffchange-inline">modified </ins>the CsgA by adding one or more His-Tag motifs, famously known to be able to chelate Nickel (<a href="http://www.nature.com/nbt/journal/v6/n11/full/nbt1188-1321.html">Hochuli 1988</a>). Our project aims at modifying an <i>Escherichia coli</i> strain that naturally produces abundant biofilm and produces the engineered curli proteins <ins class="diffchange diffchange-inline">at the same time</ins>. This way, the bacteria have the sufficient adhesion ability to stick to the filter matrix and be exposed to polluted water while chelating the environmental nickel.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td></tr>
</table>Benoitdrogue