Team:INSA-Lyon/Biology

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Revision as of 12:49, 12 October 2014

Curly'on - IGEM 2014 INSA-LYON

WETLAB SUMMARY

RESULTS

NOTEBOOK

PARTS

Our wetlab work focuses on designing a bacterial strain able to chelate as much Nickel as possible but also to adhere to a synthetic matrix for future filter design applications. To do so, we engineered a hair-shaped protein polymer located at the bacterial surface, called curli (SOURCE). CsgA, which is the monomer of this whole structure, can be engineered by our will. This property constitutes the basis of our work because we engineered the CsgA by adding one or more His-Tag motifs, famously known to be able to chelate Nickel (SOURCE). Our project aims at modifying an Escherichia coli strain that naturally produces abundant biofilm and at the same time produces the engineered curli proteins to helate Nickel. This way, the bacteria has the sufficient adhesion ability to stick to the filter matrix and be exposed to polluted water and chelate the environmental nickel.

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-Our wetlab work focuses on designing a bacterial strain able to chelates as much Nickel as possible but also to adhere to a synthetic matrix for future filter design applications. To do so, we engineered a hair-shaped protein polymer located at the bacterial surface, called curli (SOURCE). CsgA, which is the monomer of this whole structure, can be engineered by our will. This property constitutes the basis of our work because we engineered the CsgA by adding one or more His-Tag motifs, famously known to be able to chelate Nickel (SOURCE). Our project aims at modifying an <i>Escherichia coli</i> strain that naturally produces abundant biofilm and at the same time produces the engineered curli proteins to helate Nickel. This way, the bacteria has the sufficient adhesion to stick to filter
+Our wetlab work focuses on designing a bacterial strain able to chelate as much Nickel as possible but also to adhere to a synthetic matrix for future filter design applications. To do so, we engineered a hair-shaped protein polymer located at the bacterial surface, called curli (SOURCE). CsgA, which is the monomer of this whole structure, can be engineered by our will. This property constitutes the basis of our work because we engineered the CsgA by adding one or more His-Tag motifs, famously known to be able to chelate Nickel (SOURCE). Our project aims at modifying an <i>Escherichia coli</i> strain that naturally produces abundant biofilm and at the same time produces the engineered curli proteins to helate Nickel. This way, the bacteria has the sufficient adhesion ability to stick to the filter matrix and be exposed to polluted water and chelate the environmental nickel.
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