Team:IIT Delhi/Parts
From 2014.igem.org
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- | <center><h4> Biobrick 2:<a> sqr gene (sulfide quinone reductase) under constitutive promoter </a> </h4></center> | + | <center><h4> Biobrick 2:<a href="http://parts.igem.org/Part:BBa_K1395002"> Part:BBa_K1395002 sqr gene (sulfide quinone reductase) under constitutive promoter </a> </h4></center> |
<div class="vector"><img src="https://static.igem.org/mediawiki/2014/a/ad/IgemiitdS0x_clone_photo.jpg"/></div> | <div class="vector"><img src="https://static.igem.org/mediawiki/2014/a/ad/IgemiitdS0x_clone_photo.jpg"/></div> | ||
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<li>This part codes for the protein sulfide quinone reductase which is a FAD dependent oxidoreductase. Sulfide-quinone reductase (SQR),an ancient flavoprotein, is obligatory for growth on sulfide as hydrogen donor in photo and chemolithoautotrophic bacteria. It is a unique enzyme which is responsible for transfer of electrons from sulfide into the quinone pool. This enzyme converts the sulphide to Sulfur by the reaction.</li> | <li>This part codes for the protein sulfide quinone reductase which is a FAD dependent oxidoreductase. Sulfide-quinone reductase (SQR),an ancient flavoprotein, is obligatory for growth on sulfide as hydrogen donor in photo and chemolithoautotrophic bacteria. It is a unique enzyme which is responsible for transfer of electrons from sulfide into the quinone pool. This enzyme converts the sulphide to Sulfur by the reaction.</li> | ||
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- | <center><h4> Biobrick 3: <a> sqr gene (sulfide quinone reductase) under constitutive promoter </a> </h4></center> | + | <center><h4> Biobrick 3: <a http://parts.igem.org/Part:BBa_K1395003> Part:BBa_K1395003 sqr gene (sulfide quinone reductase) under constitutive promoter </a> </h4></center> |
+ | <div class="vector"><img src="https://static.igem.org/mediawiki/2014/d/da/Igemiitd_S0x_clone_Final_photo.jpg"/></div> | ||
+ | <li>This part is composite biobrick having parts of biobricks Bba_K896000 and Bba_K896001. The first part is sqr gene obtained from biobrick Bba_K896000. In this part the gene is expressed under a constitutive promoter and this enzyme converts the sulphide (S-2 ) to elemental Sulfur. And the second part is cysI gene obtained from Bba_K896001 which converts sulphite(SO32-) to sulphide (S-2 ) . So, these two parts codes for proteins which simultaneously work to convert sulphite (SO32-)to elemental Sulfur.</li> | ||
Revision as of 12:21, 16 October 2014