Team:IIT Delhi/Parts
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<h4>BioBrick:1 <a href="http://parts.igem.org/Part:BBa_K1395001"> Part:BBa_K1395001-nrfA gene (Nitrite reductase enzyme) under constitutive promoter </a></h4> | <h4>BioBrick:1 <a href="http://parts.igem.org/Part:BBa_K1395001"> Part:BBa_K1395001-nrfA gene (Nitrite reductase enzyme) under constitutive promoter </a></h4> | ||
<div class="vector"><img src="https://static.igem.org/mediawiki/2014/5/52/Igemiitd_N0x_clone_photo_%281%29.PNG"/></div> | <div class="vector"><img src="https://static.igem.org/mediawiki/2014/5/52/Igemiitd_N0x_clone_photo_%281%29.PNG"/></div> | ||
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<li>✔ This part is a combination of constitutive Promoter (BBA_J23119) and nrfA gene (BBA_k1153001). The nrfA gene (Biobrick no. BBA_k1153001) encodes for the Nitrite reductase enzyme (also known as ccNiR, source E.coli K12) which detoxifies nitrogen oxides (NOx) to ammonia (NH3). This gene was obtained from BBA_k1153001 and is 3A assembly (RFC10) compatible. The Constitutive Promoter (BBA_J23119) is the "consensus" promoter sequence and the strongest member of the constitutive promoter family developed by John Christopher Anderson of UC Berkeley.This promoter can be used to tunes the expression level of constitutively expressed parts.The nrfA gene is expressed under this constitutive promoter. Its expression is based on the availability of RNA polymerase holoenzyme and the expression is not affected by any transcription factors.</li> | <li>✔ This part is a combination of constitutive Promoter (BBA_J23119) and nrfA gene (BBA_k1153001). The nrfA gene (Biobrick no. BBA_k1153001) encodes for the Nitrite reductase enzyme (also known as ccNiR, source E.coli K12) which detoxifies nitrogen oxides (NOx) to ammonia (NH3). This gene was obtained from BBA_k1153001 and is 3A assembly (RFC10) compatible. The Constitutive Promoter (BBA_J23119) is the "consensus" promoter sequence and the strongest member of the constitutive promoter family developed by John Christopher Anderson of UC Berkeley.This promoter can be used to tunes the expression level of constitutively expressed parts.The nrfA gene is expressed under this constitutive promoter. Its expression is based on the availability of RNA polymerase holoenzyme and the expression is not affected by any transcription factors.</li> | ||
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Revision as of 11:39, 16 October 2014